Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	150.1 / NA	55	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754310‑754584                                                                                                                                         |                                                                                                                                           gCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTg                                                                                                                                      >  1:543933/1‑143 (MQ=255)  cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGc                                                                                                                                     <  1:894408/143‑1 (MQ=255)   aGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                    <  2:56234/143‑1 (MQ=255)   aGGATGCGGTTGAAGCGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGGTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                    <  2:907711/143‑1 (MQ=255)    ggATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTTGGTTTTGcat                                                                                                                                   >  1:501570/1‑141 (MQ=255)          gtgtcaagtgcagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGACATCCATAACATCGTGACCCAGCGCGCCTTTCAGCACATCCAGTTCAACAGCTGGATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAt                                                                                                                             <  2:816867/138‑1 (MQ=255)                 gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTCTGTATCAGCCAt                                                                                                                      >  1:279228/1‑143 (MQ=255)                     agccgtggTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGAGATCAATCACATCTTGACCCAGCGTGCCTTTCAGCACATCTCGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                                  <  2:734566/137‑1 (MQ=255)                        cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGt                                                                                                               >  1:974789/1‑143 (MQ=255)                        cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGt                                                                                                               >  1:45436/1‑143 (MQ=255)                               aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                        >  1:516407/1‑143 (MQ=255)                               aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTGCCCGCACATCCAGTTCAACAGCTGTATCCCCGTTGGGGGTGGGGTTCGCTGGCGCATCAGCACTTCAAGGTCTCCATa                                                                                                        >  2:867702/1‑143 (MQ=255)                                 aGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgc                                                                                                      >  1:636509/1‑143 (MQ=255)                                         acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTATTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  2:66273/143‑1 (MQ=255)                                             cTTTTGAGCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                          <  2:515834/143‑1 (MQ=255)                                              ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGGGGGTCGGTCTTGCTGTTTTATCAGCCACTTAAGGTCTCCTTCGCGCCTTATTGCGtc                                                                                         >  1:39730/1‑142 (MQ=255)                                               ttttAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        <  1:508702/143‑1 (MQ=255)                                                 tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                      <  2:107404/143‑1 (MQ=255)                                                   agccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTACGGTCTCCTTAGCGCCTTATTGCGTAAGACt                                                                                    >  2:768916/3‑143 (MQ=255)                                                    aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTg                                                                                   >  2:584600/1‑143 (MQ=255)                                                    aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTCTGCTGTTGTATCACCCATTTCAGGTCTCCGTGTCGTCTTGTTGCCTAacaccg                                                                                   >  2:857800/1‑141 (MQ=255)                                                      cGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCCTTGAGGGTGGGTTTCGCTTTTGTTTCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCCTACGACTGcc                                                                                 >  2:121203/1‑143 (MQ=255)                                                        agagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCgg                                                                               >  2:389524/1‑143 (MQ=255)                                                                   attaaTAACAGCTTGTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                    <  2:543933/140‑1 (MQ=255)                                                                     cactAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCTCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                  <  2:371124/140‑1 (MQ=255)                                                                        tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               >  1:973561/1‑143 (MQ=255)                                                                         aaCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCttt                                                              >  1:115838/1‑142 (MQ=255)                                                                           cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCTTTGAGGGTGGTTTTTTCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGc                                                            <  1:620486/143‑1 (MQ=255)                                                                                 gACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTACATTTGCCTTCGCACATcc                                                      >  2:205350/1‑142 (MQ=255)                                                                                    ccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCCAATCAAcc                                                   >  2:100917/1‑143 (MQ=255)                                                                                    ccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAcc                                                   >  1:758441/1‑143 (MQ=255)                                                                                              tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAca                                         >  2:28846/1‑142 (MQ=255)                                                                                               ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAccc                                        >  1:573841/1‑143 (MQ=255)                                                                                                tCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACCTAAATTTGCCTTCGCACATCAACCCGGCTTTACCCg                                       >  2:22082/1‑143 (MQ=255)                                                                                                   gCACAGCCAGTTCAACAGCTGTATTCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                    <  1:932803/143‑1 (MQ=255)                                                                                                     acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGACGGAACTTAAATTTGCCTCCGCACATAAACCTAGCTTTACCCGttttt                                  >  2:324774/1‑143 (MQ=255)                                                                                                     acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTCAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttttt                                  >  1:881908/1‑143 (MQ=255)                                                                                                      caTCCAGTTCAACAGCTGTATCCTCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                                 <  2:616978/143‑1 (MQ=255)                                                                                                       aTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                <  1:584600/143‑1 (MQ=255)                                                                                                            ggtCAACAGCTGTATCCCCGTGGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                           <  2:420484/141‑1 (MQ=255)                                                                                                                aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCCTTTTTTATTTGGCTcc                       >  2:649017/1‑142 (MQ=255)                                                                                                                aaCAGCTGTACCCCCGTTGAGGGTGGGTTTTGCTCTAGTGTCAGCCGGGTAAGGTCTCCTTAGCGCCGTATTGCGTAAGACTGCCCGACCTTAATTTTGCCTTCGCACTTCATCCTGGCTTTACCCGTTTTTTATTTGGCTcg                       <  1:955897/143‑1 (MQ=255)                                                                                                                   aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                    >  2:567072/1‑143 (MQ=255)                                                                                                                       gTATCCCCGTGGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGctct                <  1:95519/143‑1 (MQ=255)                                                                                                                        tATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGACATCGGAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtg               <  1:601874/143‑1 (MQ=255)                                                                                                                         tgccccGTTGAGGGGGGTTTGTGTTGTTGGAGCAGCCATGTGAGGTCTCCTTAGCGCCTTAGTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:279228/141‑1 (MQ=255)                                                                                                                         aTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:187116/143‑1 (MQ=255)                                                                                                                         aTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  1:891925/143‑1 (MQ=255)                                                                                                                             ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCACACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa          >  1:294346/1‑143 (MQ=255)                                                                                                                                 tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTATATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:330696/143‑1 (MQ=255)                                                                                                                                 tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:45436/143‑1 (MQ=255)                                                                                                                                 tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  1:542988/143‑1 (MQ=255)                                                                                                                                 tGAGGGTCGGTTTTGCTTTTGTGTCTGCCATTTAGGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAGCTTATATTTGCCTGCGCACATCAACCTGGCTTTCCACGTTTTTAATGTTTCTCGCCGCTCTGTGAAAGAgg      <  2:130755/143‑1 (MQ=255)                                                                                                                                  gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg     <  1:795487/143‑1 (MQ=255)                                                                                                                                     ggTGGGTTGTGCTTTTGTATCAGCCGTTTAAGGTCTCCGTAGCGCCCTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACAGCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  <  2:637444/143‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754310‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑