| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | 100% | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 98.0% | 110.3 / ‑5.8 | 45 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (21/22); minor base C (2/0); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT > NC_000913/760353‑760619 | gagaTGAAGCTCCTGCAGGCGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGa < 2:241111/143‑1 (MQ=255) aTGAAGCGGCTGGAGTGGCTGGCGAAACGCATGAGCGCGGTGGGGGGGGCAGTTGAAATGCAGTCTCGCGGTGCCAAGGTGTATGGCGATCGCCAGGCGATGGGTGCCGGTGAGAAACTGGTCGACTGGAGCGGTGCGGaaaa < 1:447988/143‑1 (MQ=255) tGAAGCCCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAc < 1:1031512/143‑1 (MQ=255) aaGCGCCTGCAGCAGCTGGCGTAACGCATCAGCGCGGTGCCGGAAGCAGGTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCt < 1:900255/143‑1 (MQ=255) ggcgtCTGCAGCAGCGGGCGAAAGGGCTCAGCACGGTGCCGGAAGTATGTGGAATGCAGTTTCGCGTTGCCAAGCTTTATTGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 2:425952/142‑1 (MQ=255) agaggcTGCAGGAGCTGGCGAAACGCAACAGAACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 1:10579/138‑1 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg < 1:170296/143‑1 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGGGCGGAAAAcaccg > 2:599038/1‑139 (MQ=255) gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGGTGGCAGGATTTATGGCGAGCGCCAGGCGAAGGCTGCCGGTGAGAAACTGCTCGACTGGAGCGGTGCGGAAAACCtgg > 2:953288/1‑141 (MQ=255) cgcCTGCGGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGCTGAAATGCAGTCTCGCGTTGCCAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGAGTGGAGCGGTGCGGCAAACCTCGc > 2:838529/1‑143 (MQ=255) cgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGTCTGGCGCGGTGCGGAAAAcaacgc > 1:886023/1‑138 (MQ=255) cgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGc < 1:625084/143‑1 (MQ=255) cTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCACGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAGAACCTCGCTTa > 2:666334/1‑143 (MQ=255) tGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGCTGGCTGACGGTGAGAAACTGATAAACAGGAGCAGTACAGAAAACCTGGATTAc > 1:263686/1‑143 (MQ=255) ggaGGGGCTGGCGAAACGCATCTGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg < 2:355287/141‑1 (MQ=255) ggAGCGGGCGAAACGCCTCAGCACGGTGCCGGAAGCAGTTGACATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCa < 2:324387/143‑1 (MQ=255) gAGCTTGCGAAGTTCATGAGGACGGTGCCGGACGCAGTTGAAGTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGTGGTGCGGAAAACCTCGCTTACGCCAc < 2:920109/143‑1 (MQ=255) aGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGAGCGGAAGACCTCGCTTACGCCCCg > 1:687192/1‑143 (MQ=255) tGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTg > 1:1014433/1‑143 (MQ=255) cGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGtt > 1:695703/1‑143 (MQ=255) gAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTtg > 1:765562/1‑143 (MQ=255) aCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg < 1:674360/143‑1 (MQ=255) gCATCAGCACGGTGCCGGAAGCAGTAGAAATGCAGACTCGAGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACAGATCGACTGGAACGGCGCGGAAAACCTCGCATACGCAACGCTGGATGATGaa > 2:329706/1‑143 (MQ=255) tGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATGCCGGtt < 2:817751/143‑1 (MQ=255) gggTTGTACTGCCGTCTGGCGTGGCCATGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCg < 2:357918/141‑1 (MQ=255) gtGAAATGCAGGCGCGCGTTGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGTTCGCCTGTCGGGt < 1:19507/142‑1 (MQ=255) aaaTGCAGTCTCGCGTTGTCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggctcaa > 2:279843/1‑138 (MQ=255) gcgTCTCGCGTTGCCAAGCTTTATGGCGAGGGCCAGGCGATGGCTTCCGGTGAGAAACTGTTCGAGTGTAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTc < 2:42001/141‑1 (MQ=255) cgcgTTGCGAAGCTTTGTGGCGATCGCCAGGCGAGGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcg < 2:902988/143‑1 (MQ=255) gTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGGTCGACTGTAGCGGTGCGGAAAACCTCGCTTACGCTATGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCgg < 1:180475/143‑1 (MQ=255) gCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGAGAGAAACTGTGCGACTGGCGCGGTGCGGGACACCGCGCTTGCCCCACGCTGGTGGATGAAGGCAGTCCGGTTCGGCTGGCACGCGAAGACCCAGGTGGCGGtgc > 2:162961/1‑141 (MQ=255) ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGCAGACTCCGGTCGCGGTAcc > 2:652999/1‑143 (MQ=255) ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAcc > 1:289541/1‑143 (MQ=255) cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTGGCAGATAGGGTGCAGAGGCCGGTCGCGGTACct > 2:730318/1‑143 (MQ=255) tttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc < 2:618206/143‑1 (MQ=255) gctCGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTGCGCAACGCTGGTTGATGAAGGCATTCCGGGTCGCCAGTCGGGTGAAGACTCCGGGCGCGGTACCCTCCTCCACCGccc > 1:173760/3‑142 (MQ=255) aTGTCTGCCGGTGAGGAGCTGCGCGATCGGAGCGGTGCGGAAAGCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCca < 2:964168/143‑1 (MQ=255) cTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc > 1:463040/1‑143 (MQ=255) gCCGGCGAGAAACTGTTCGACTGGAGCGGCGCGGAACACCCCGCTCACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCCGTCGCGTGAAGACTACGGTAGCGCTACCTCATTCCCCCGCCACACGGCGACCCCccacca > 2:520565/1‑143 (MQ=255) ccGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGGGATCCACAACCAg > 1:739910/1‑143 (MQ=255) cGGTGGGAAGCTGGTCGACTGGAGCGGTGCGGCAACCCTCGCTTATGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGt < 2:36925/143‑1 (MQ=255) cGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGt > 1:968980/1‑143 (MQ=255) gagaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGACATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCCCCACGCGGTGATCCACAACCAGtgaaa > 1:1071438/1‑139 (MQ=255) aCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTCGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt < 1:875550/143‑1 (MQ=255) tCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGGTCCACAACCAGTCTACCGGTTCCaat > 1:83358/1‑141 (MQ=255) | GAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT > NC_000913/760353‑760619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |