| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 781,668 | C→T | 12.0% | intergenic (+16/‑417) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,668 | 0 | C | T | 12.0% | 89.2 / 8.9 | 50 | intergenic (+16/‑417) | lysQ/nadA | tRNA‑Lys/quinolinate synthase |
| Reads supporting (aligned to +/- strand): ref base C (28/16); new base T (3/3); total (31/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-02 | |||||||||||
CTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAT > NC_000913/781529‑781810 | cTGCACGACCCAGCAGCCTGGAGGATCACGGCAGCATAACCCCGCTAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTGTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa < 2:534979/143‑1 (MQ=17) gACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGCGGTCGTTAGCTCAGTTGGTAGAGCAGTCGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcgc > 2:478471/1‑143 (MQ=255) cccACCATCCTGAATGATTAAGGCAGCAAAAACCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCtt < 1:397170/143‑1 (MQ=18) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCcttt > 1:616081/1‑143 (MQ=255) caTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTg > 2:854893/1‑143 (MQ=255) aTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCttttttac < 1:634356/143‑3 (MQ=17) ccTTAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCttttttacat < 1:1015003/143‑5 (MQ=17) aaTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTACTCAATTGGTCGCAGGTTCGAATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTCACTCTCTg > 2:720190/1‑143 (MQ=17) aaTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGGCCCACCAATGTAAACAAGCGCCCTAAAGGCGCTATTTTGCTATCTg > 2:825944/1‑143 (MQ=255) tGATTAAGGCAGCAGAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCg < 1:996454/143‑1 (MQ=255) ggCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACCCa > 2:319706/1‑143 (MQ=255) gcggcaTAACCGCGGAAGCGGTCGTTCGCTCAGTTGGTAGAGCAGTCGACTTTTCATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa < 2:638056/140‑1 (MQ=11) tAACCCCGTAATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc < 2:452731/143‑1 (MQ=255) aTCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGa < 1:179938/143‑1 (MQ=40) tCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCGTGCACGACCCACCAATGTAAAAAAGCGCCCTAAGGGCGCTTTTTTGCTATCTGCGGTACCCAACGATTCGaa > 1:506871/1‑143 (MQ=14) cGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTGAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCt > 2:764062/1‑143 (MQ=37) gCACGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTg < 2:751320/143‑1 (MQ=21) gggggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgcag < 1:20884/142‑1 (MQ=18) atgggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgcag < 2:973269/141‑1 (MQ=17) ggggTCGTTAGCTCAATTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGACTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTGTTTGCTATCTGCGATACTCAAAGCTACGAACCTgaagc > 2:902700/1‑143 (MQ=11) ggtggCTCAGTTGGTAGAGGAGTTGACTTTGAATCAGGGGGGCGCAGGTTCGAATCGTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCTGCAGGttc < 2:67817/139‑2 (MQ=11) agtgcggTTGGTAGATCTGTTGACTTGTAATCGATTGTTCGCAGGTTCGAATCCTGCACGGCGCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgag < 2:809487/137‑1 (MQ=11) gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAGAAAGCGCCCTAAAGGCGCTTCTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgc > 2:949960/1‑143 (MQ=17) gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa < 1:894304/143‑1 (MQ=32) cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaac > 1:865767/1‑143 (MQ=32) gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaa > 2:326649/1‑143 (MQ=32) ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGc > 2:112891/1‑143 (MQ=32) tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAATGTAACAAAGCGCCCTACCCGCGCTTTTTTGCTATCTGCGATCCTCAAAGATACGAACATGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGAAGcc > 1:225393/1‑143 (MQ=11) tattCAATTGTTCGCAGGTACGAATCGTAGACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTGTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGc < 1:117259/140‑1 (MQ=11) aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTc > 1:464577/1‑143 (MQ=32) aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg > 1:548323/1‑143 (MQ=32) ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc > 1:694776/1‑143 (MQ=32) gAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTACGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCACGCGGCGACGGCCCGAAagg > 2:322106/1‑143 (MQ=12) aagccTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTGTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc < 1:1013458/140‑1 (MQ=17) cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTGGAGTTGAACGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaa > 2:525764/1‑143 (MQ=17) gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACAGAGCCGCTAACGACGACGGCCCGAAGGGCGagagaagcg > 1:462236/1‑143 (MQ=11) aCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTCGAATTGAACGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 1:902585/143‑1 (MQ=12) caccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGGAACAACGGAGCCGCTCGCGGCGACGGCCCGGAGGGCGAGCGACGCGAGTc > 2:542127/1‑143 (MQ=12) accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGCGTCa > 1:385679/1‑143 (MQ=17) accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCa > 2:252245/1‑143 (MQ=32) tGTAAAAAAGCACCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTg > 2:908406/1‑143 (MQ=17) gTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCCGCGAAACAACGGCGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGAGACGCATCCTGc > 1:301760/1‑143 (MQ=11) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCCTCCTGCAc > 2:859799/1‑143 (MQ=11) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc > 2:338047/1‑143 (MQ=255) aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGAAACGCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc > 2:647309/1‑143 (MQ=11) aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg > 1:143950/1‑143 (MQ=32) aaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGGCATCCTGCACGAcc > 2:908716/1‑143 (MQ=17) gcgcCCTAAGGGCGCTTTTTTGCTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGACGCAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca < 2:357066/143‑1 (MQ=255) gcgcCCTAAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca < 2:244911/143‑1 (MQ=17) gcCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTGCAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGACGGGCGAGCGCAGCGAGTCATCCGGCACGAcccccc > 2:681155/1‑143 (MQ=11) cccTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca > 2:351949/1‑143 (MQ=32) ccTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa > 1:703898/1‑143 (MQ=32) ccTAAAGGCGCTTTTGTGCTATCTGCGATTCTCTATGATACGAACCTGGAGCCGGTTGGAGTCGAGCGCAGCGAAACAACGGAGCCCCTCGCGGCCACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa < 2:18391/143‑1 (MQ=11) cTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt < 1:650336/143‑1 (MQ=32) | CTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAT > NC_000913/781529‑781810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |