BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,220	C→T	100%	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	94.7 / NA	38	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/23); total (15/23)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196087‑1196312

tcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTgg                                                                                     <  1:921641/143‑1 (MQ=255)

 cgcgCCTGGTGCAAACGTCGGTGACGAATGCGCCCTGTTGGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TAGGCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGa                                                                                    <  2:157002/143‑1 (MQ=255)

  gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGAc                                                                                   >  1:316068/1‑143 (MQ=255)

     ccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTATATGGAAAGAGTGTG‑TGAGATGATGCTGCGCCATCTGGGTTGGACTGa                                                                                >  2:220922/1‑143 (MQ=255)

      cTGGTGCAAACAGCGGTGACGAATGCGCCCTGCTTGAAGCCACCCACGGGACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa                                                                               <  2:85240/143‑1 (MQ=255)

            cAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCt                                                                         >  2:254530/1‑143 (MQ=255)

                    ggTAACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                                 <  2:619867/143‑6 (MQ=38)

                         cggttGCGTCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  2:395629/139‑1 (MQ=38)

                         cGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACCAAGTACATCCTGGCTCTAT‑TGTTCGCTCCGTTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  2:676700/143‑1 (MQ=37)

                           aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTATAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                          <  2:500941/143‑1 (MQ=38)

                            aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                         >  1:751269/1‑143 (MQ=255)

                             ggcgcCCTGTTTAAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                        <  1:1069071/142‑1 (MQ=37)

                                 cccTGTTTGAAGGCACCCTCGGTACTGCGCCGAAATCTGCCGGTCAGGACACAGTAAAGCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATg                                                    <  1:908850/143‑1 (MQ=25)

                                    tggttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                                                 <  1:7862/140‑1 (MQ=37)

                                    tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGCTGACCTGATTGTTACAGGTATGGaa                                                 >  1:37862/1‑143 (MQ=37)

                                    tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGGAAATCCTGGCTCTAT‑TATTCTCTCCGCGGAGATGATGCTGCGCCATATGGGTTGGGCTGAAGCGGCTGAGCTGACGGTTAAAGGGATGGaa                                                 >  2:28130/1‑143 (MQ=37)

                                         gAAGCCACCCACGGTACTGCGCCGAACTATGGCCGTCAGCACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGATATGATACTTCGCCATATGGATTCGACGGAGGCGCCGGACCTCAATCTTAAAGGTATGGAAGgcgc                                            >  2:523169/1‑143 (MQ=255)

                                           aGCCACCCGTGGCACTGCCCCTAAAGATGCCGGTCAGGACAAAGTAAATCCTGGGTCTAG‑TACTTTCTCCGCTGAGATTATTCTGTGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                                          <  2:166237/143‑1 (MQ=17)

                                           aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCAGAGATAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa                                          <  1:673933/143‑1 (MQ=25)

                                                cccacGGTACTGCGCCGAAATATGCCAGTCAGAACAAATTAAGTCCTGGCTCTAT‑TATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                     <  1:443585/143‑2 (MQ=18)

                                                 gcacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg                                    <  1:1036081/142‑3 (MQ=25)

                                                  cacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc                                   <  1:71016/143‑1 (MQ=21)

                                                     ggTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa                                <  1:542731/143‑7 (MQ=21)

                                                      gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag                               >  1:525706/1‑142 (MQ=21)

                                                      gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTGAAAGGTATGGGAGGCGCAATCAATGCCaag                               >  2:137970/1‑142 (MQ=21)

                                                      gTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaag                               <  1:937945/143‑8 (MQ=18)

                                                        aCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             <  2:906732/143‑1 (MQ=18)

                                                         cTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCCGATTGTTACAGGGATGGAAGGCGCAATCAATGCCAAGAcc                            >  2:838275/1‑143 (MQ=21)

                                                           gcgcCGAAATATACCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt                          <  1:883611/143‑1 (MQ=18)

                                                               cGAAATATGCCGGTCAGGACAAAGTAAATCCGGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct                      >  2:938155/1‑142 (MQ=17)

                                                                       gCCTGTCAGCACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGTACTGAAGCGGCTGACCTTATTTTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt              <  1:632423/143‑1 (MQ=17)

                                                                       gCCGGTCAGGACAAAGTAGATCCGGGCTCTAT‑TATTCTCTCCGCTGGGATGATGCTGCTCCATATGGGTTGGAGTGAAGCGGCTGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt              <  2:269061/143‑1 (MQ=14)

                                                                        ccGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTCTGACTTc             >  1:464001/1‑143 (MQ=17)

                                                                         cGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg            >  1:998018/1‑143 (MQ=14)

                                                                           gTCAGGATAAAGCAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaa          <  1:753236/143‑2 (MQ=11)

                                                                                gACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt     >  2:581987/1‑143 (MQ=14)

                                                                                gACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt     >  2:232251/1‑143 (MQ=14)

                                                                                   aaaGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat  <  1:808122/143‑1 (MQ=11)

TCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196087‑1196312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`