| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,196,232 | C→T | 100% | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,232 | 0 | C | T | 100.0% | 87.0 / ‑6.7 | 35 | T370T (ACC→ACT) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (13/21); minor base G (1/0); total (14/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT > NC_000913/1196092‑1196312 | ccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTATATGGAAAGAGTGTG‑TGAGATGATGCTGCGCCATCTGGGTTGGACTGa > 2:220922/1‑143 (MQ=255) cTGGTGCAAACAGCGGTGACGAATGCGCCCTGCTTGAAGCCACCCACGGGACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa < 2:85240/143‑1 (MQ=255) cAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCt > 2:254530/1‑143 (MQ=255) ggTAACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat < 2:619867/143‑6 (MQ=38) cggttGCGTCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa < 2:395629/139‑1 (MQ=38) cGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACCAAGTACATCCTGGCTCTAT‑TGTTCGCTCCGTTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTa < 2:676700/143‑1 (MQ=37) aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTATAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa < 2:500941/143‑1 (MQ=38) aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg > 1:751269/1‑143 (MQ=255) ggcgcCCTGTTTAAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg < 1:1069071/142‑1 (MQ=37) cccTGTTTGAAGGCACCCTCGGTACTGCGCCGAAATCTGCCGGTCAGGACACAGTAAAGCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATg < 1:908850/143‑1 (MQ=25) tggttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa < 1:7862/140‑1 (MQ=37) tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGCTGACCTGATTGTTACAGGTATGGaa > 1:37862/1‑143 (MQ=37) tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGGAAATCCTGGCTCTAT‑TATTCTCTCCGCGGAGATGATGCTGCGCCATATGGGTTGGGCTGAAGCGGCTGAGCTGACGGTTAAAGGGATGGaa > 2:28130/1‑143 (MQ=37) gAAGCCACCCACGGTACTGCGCCGAACTATGGCCGTCAGCACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGATATGATACTTCGCCATATGGATTCGACGGAGGCGCCGGACCTCAATCTTAAAGGTATGGAAGgcgc > 2:523169/1‑143 (MQ=255) aGCCACCCGTGGCACTGCCCCTAAAGATGCCGGTCAGGACAAAGTAAATCCTGGGTCTAG‑TACTTTCTCCGCTGAGATTATTCTGTGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa < 2:166237/143‑1 (MQ=17) aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCAGAGATAATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa < 1:673933/143‑1 (MQ=25) cccacGGTACTGCGCCGAAATATGCCAGTCAGAACAAATTAAGTCCTGGCTCTAT‑TATTCGCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat < 1:443585/143‑2 (MQ=18) gcacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg < 1:1036081/142‑3 (MQ=25) cacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc < 1:71016/143‑1 (MQ=21) ggTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa < 1:542731/143‑7 (MQ=21) gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag > 1:525706/1‑142 (MQ=21) gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTGAAAGGTATGGGAGGCGCAATCAATGCCaag > 2:137970/1‑142 (MQ=21) gTACCGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaag < 1:937945/143‑8 (MQ=18) aCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc < 2:906732/143‑1 (MQ=18) cTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCCGATTGTTACAGGGATGGAAGGCGCAATCAATGCCAAGAcc > 2:838275/1‑143 (MQ=21) gcgcCGAAATATACCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt < 1:883611/143‑1 (MQ=18) cGAAATATGCCGGTCAGGACAAAGTAAATCCGGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct > 2:938155/1‑142 (MQ=17) gCCTGTCAGCACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGTACTGAAGCGGCTGACCTTATTTTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt < 1:632423/143‑1 (MQ=17) gCCGGTCAGGACAAAGTAGATCCGGGCTCTAT‑TATTCTCTCCGCTGGGATGATGCTGCTCCATATGGGTTGGAGTGAAGCGGCTGACCTGAGTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt < 2:269061/143‑1 (MQ=14) ccGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTCTGACTTc > 1:464001/1‑143 (MQ=17) cGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg > 1:998018/1‑143 (MQ=14) gTCAGGATAAAGCAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaa < 1:753236/143‑2 (MQ=11) gACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 2:581987/1‑143 (MQ=14) gACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 2:232251/1‑143 (MQ=14) aaaGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat < 1:808122/143‑1 (MQ=11) | CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTAT‑TATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT > NC_000913/1196092‑1196312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |