| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 99.3% | 135.5 / ‑6.6 | 56 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (23/29); minor base G (1/1); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTC > NC_000913/124099‑124379 | ccGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACCGTAATCCTTGCTCATACCATTAAAGGTGACGGCATGAGCGACGCGGCTGAAGGCAAAAACCTCCCGCAcc > 2:461757/1‑143 (MQ=255) tgggcgccACGATCCGAAGAAAATCTACGCCGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAgg < 1:499782/136‑1 (MQ=255) ggtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCCGAAGGTAAAAACATCGCGCACGAGGTTaa > 2:306330/1‑143 (MQ=255) cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGCCGCGGCCGCAGGTAAAAACATCGCGCACTAGGTTAAGac > 1:574321/1‑142 (MQ=255) cGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGACATGGGCGACGCGGCTGAAGGTAAAAACATCGCGAACAAGGTTAAGaaaa > 1:532528/1‑143 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 2:457350/143‑1 (MQ=255) aTCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTTATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATg < 2:929721/143‑1 (MQ=255) gtcGAAGGAAATCTATGCTGCACTCAAGCAAGCTCAGGAAACCAGAGGCAAAGCGACAGTAATCCTTGTTCATACCATTAAAGGGTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCATTAGGTTAAGAAAATGa < 2:461940/141‑1 (MQ=255) cGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAAc > 1:393170/1‑143 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACa > 1:297938/1‑143 (MQ=255) gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGTATAAGAAAATGAACATgg > 2:506254/1‑143 (MQ=255) aaaTCTACGCTGCATTCACGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTCAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAc > 1:418452/1‑143 (MQ=255) tACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt > 1:44592/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGGCGCGGCTGAAGGTAAAAACATCGCGCACTGGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATcgg > 1:146604/1‑141 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg > 1:330682/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg < 1:1020509/143‑1 (MQ=255) aaaGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGa < 1:494840/143‑1 (MQ=255) aGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACCGTGTGCGTCATGTcaaggacc > 1:464293/1‑136 (MQ=255) ccAAATGCAATGCGACAGTAATCCTTGCTCATCCCATTAAAGGTTACGGCATGGGCGATGCGGCTGAAGGTAAAACCATCGCGCATTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAtgtg < 2:574321/143‑1 (MQ=255) cAAAGGCAAAGCGACAGGAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc < 2:815911/143‑1 (MQ=255) aaGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 2:974490/143‑1 (MQ=255) aaGGCAAACCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 2:175717/143‑1 (MQ=255) gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGa < 2:297938/143‑1 (MQ=255) gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCAGGGGCGACGCGGCTGAAGGTAAAACCATCGCGCTCTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGGTTCAATGTGCCGGAGGCTGa > 2:965689/1‑143 (MQ=255) ctataGTAAATCTTGCTCACAACATTAAAGGTCAGCGCATGGCCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATAGCCGCGACCGTTTCAATGTGCCGGTGTCTGAt < 1:398401/139‑1 (MQ=255) aCAGTAATCGTGGCACATACAATTAAAGGTGACGGGAGGGGGGACGCGGCGGAAGGTAAAAACATCGCGCACTAGGTTGAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGc < 2:56810/143‑1 (MQ=255) aCAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGc > 1:1040585/1‑143 (MQ=255) gTAATCCTTGCTCATACCATTACAGGTTACGGCATGGGCGACGCGGCGGGCGGTAAAAACATCGCGCACTAGGTTAAGATAATGAACATGGACGGTGTGCGTCATAGCCGCGACCGGTTCAATGTGCCCGTAGCTGATGCaca > 2:350471/1‑141 (MQ=255) tAATCCTTGCTCATACCATTAACGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTCCAATGTGCCGGTGTCTGCTGCAGac > 2:466349/1‑142 (MQ=255) tAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGAGACAAGCGCCCTACCGTTACAATGTGCCGGTGTCTGATGCAgcg > 2:753089/1‑141 (MQ=255) aTCCTTGCTCCTACCATAAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGCCCGTTTCAATGTGCCGGTGTCTGATGCAGatat < 2:135889/143‑1 (MQ=255) cTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGa > 2:681508/1‑143 (MQ=255) gggtACGGCAGGGGCGACGCGGCTGAAGGTAAAACCGTCGCGCACTAGGTTAAGAAAATGACCGTGGACGGTGTGCGTGATATCCGCGACCGTTTCAACGTGCCGGTGTCGGATGCAGATATCGAAAAACTGCCGTACATCAc < 2:326318/140‑1 (MQ=255) ggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAc < 2:648758/143‑1 (MQ=255) ggTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAc < 1:612582/143‑1 (MQ=255) gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCAcc > 2:1051246/1‑143 (MQ=255) ggCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGACACACTGCCGTACATCCCCTTccc > 1:321093/1‑143 (MQ=255) tGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATGTCGAACAACTGCCGTACATCACCTTCCCGGag > 1:484121/1‑142 (MQ=255) cgggCGACGCCGCTGACGGGAAAAACCTCGCGCACTAGGTTAAGAAAACGAACATGGACCGTGTGCCGCATATCCGCGACCGTGTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGaa < 1:67331/142‑1 (MQ=255) gggCGACGCGGCTGGAGTTACAAACATGGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAg < 2:722022/143‑1 (MQ=255) gggCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATCTCGAAAAACTGCCGTACATCACCTTCCCGGacg > 1:171733/1‑141 (MQ=255) gcccgggCTGAAGGTAAAACCATCGCCCACTAGGTTAAGAAAATGAACATGGACGGTGTGCCTCATATCCGCGACCGTTTCAATGTGCCGGGGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCGGGAAGGtga < 1:216987/138‑3 (MQ=255) ccgcgGCTGAAGGTAAAAACATCGCGCACTAGGTTAACAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCGGGAAGGtgat < 2:490858/142‑4 (MQ=255) aCGCGGCTGAAGGTAAAAACATCGCGCAGTAGGTTAAGAAAATGAACATGGACGGTGCGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGAGGCAGAGATCGAAAAACTGCCGTACATCCCCTTCCCCGAAGGctct > 2:212217/1‑143 (MQ=255) gcggatggTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGAGATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 2:413976/137‑1 (MQ=255) cTGAAGGTAAAAACGTCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag < 2:492472/143‑1 (MQ=255) aaGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGCAGGTTCTGAAGTGCAt > 1:1040310/1‑143 (MQ=255) ggggaaaaaCATCGCGCACGAGGTGAAGAAAGTGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAAGGTGCCGGTGTCTGATGCAGATATCGAAAACCTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 2:378768/139‑1 (MQ=255) aGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAGGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 1:209931/143‑1 (MQ=255) gTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGGTGCAGATAAGTAAATGCGGCCCTAGATCATCTTCGCGGCAGCGTCTGGAGAGGATAcc > 1:1063872/1‑143 (MQ=255) aaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATc < 1:1051246/143‑1 (MQ=255) aaCATCGCGCGCTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCt < 1:703821/143‑1 (MQ=255) tCGCGCACTTGTTTAATAAGCGGAACTGGGACTGTTTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATACCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCAc < 2:532528/143‑1 (MQ=255) tCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCAc > 1:634234/1‑143 (MQ=255) tCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCAc > 1:632731/1‑143 (MQ=255) cgcgCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGACATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACg < 1:601875/143‑1 (MQ=255) gcACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTc < 1:506254/143‑1 (MQ=255) | CCGTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTC > NC_000913/124099‑124379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |