| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,895,391 | A→T | 11.6% | K196I (AAA→ATA) ‡ | pabB → | aminodeoxychorismate synthase subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,895,391 | 0 | A | T | 11.6% | 72.3 / 3.9 | 37 | K196I (AAA→ATA) ‡ | pabB | aminodeoxychorismate synthase subunit 1 |
| Reads supporting (aligned to +/- strand): ref base A (19/12); new base T (2/2); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01 | |||||||||||
TCATACAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCGCGCGCCATTTAGCGCT > NC_000913/1895251‑1895533 | tCATACAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGCCCCGCGAGCAGTACGGCGaaaact > 1:191562/1‑141 (MQ=255)tCATACAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATACGACCCGCGACCAGTACGGCGCAAAAt > 2:895991/1‑143 (MQ=255) aTACAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAACCCAATATGACCTGCGCTCAGGCCGGCGAAAAAttt > 2:418434/1‑143 (MQ=255) aCAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCg > 1:521320/1‑143 (MQ=255) cAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCGATTCTCGCCGCGGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGCAACACTTCGc > 1:215558/1‑143 (MQ=255) ttCTTTGCTGAGTCCTAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAATTTTTCGccgta < 1:533360/143‑4 (MQ=255) cctAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCt < 1:727466/141‑1 (MQ=255) cATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCt < 2:357092/143‑1 (MQ=255) gCCCTTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTGCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTg < 1:51021/143‑1 (MQ=255) ccGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCACTATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTCCAGGAATATCTGCCCCGCGGCGCTTGcc > 1:384591/1‑142 (MQ=255) ccGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAAATTCGCCAGATACAGGAATATCTGCACAGCAGTGAATGCt > 1:957554/1‑143 (MQ=255) ggCCTGCGTGGACGGCGAGCAATTCCCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGCCCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAgg < 1:718472/143‑1 (MQ=255) ccTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCCATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATGGCTATCAggag > 2:354568/1‑141 (MQ=255) ccTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATTTGACCCGCGTGTTGTTTGCACAAAAACCCCCCCCGGTACTGTAATATCTGCACATCCGTCACTGCCATCAGGta > 1:242939/1‑142 (MQ=255) ccTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTAACAGGTg > 2:873293/1‑143 (MQ=255) tggAAAGCTAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCCGTACGGCGAAAAATTTCGCCAGGTACGGGAGCCTCCGCACCGCGGTGATTGCTGTCAGGTGACtctc > 1:218090/1‑143 (MQ=255) tggAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGGAtccc > 2:890755/1‑141 (MQ=255) aaaGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAACAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGcc > 2:798299/1‑143 (MQ=255) ggtggCAATTCGCGCCGTAGGAAGATTTCACTCTCACTTCCGACTGGCAATCCAGTATGACCCGCGAGCAGTACGGCGAAAGATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCaa < 2:854975/139‑1 (MQ=255) gCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGtt > 1:518422/1‑143 (MQ=255) aaTTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGtttt < 2:359782/143‑1 (MQ=255) tctcGCCGCAGGAAGGTGTCACGCTCACTTCCGACTGGCAATCCAATATGACGCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCAt < 1:49039/143‑1 (MQ=255) tcccgccgcAGGGTGATTGCACGCTCGCCTCCGCCTGGCCATCCAATATGTCCCGCGAGCGGTACGGCGAAAAGTTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCAAACAACTTTTTCAt < 2:999913/140‑1 (MQ=255) tgacgtCCGACTGGCAATCCAATATGACTCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATgg < 2:583980/138‑1 (MQ=255) tCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATgg > 1:843562/1‑143 (MQ=255) tCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAAAATCTGAACAGCGGTGGTTGCTCTCAGGCGAATCTCGCCCAACGCTTCCATGCGACCTACTCGCGCGATGGAtag > 1:1067991/1‑141 (MQ=255) cTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCTGGTACAGAAGTAGCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATggcag < 1:667397/143‑1 (MQ=255) gACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTCTCCATGCGACCTATTATGGCGGTGAATGGCAGGCAtt > 2:1035589/1‑143 (MQ=255) ggCAATCCAAGATGGCCCGCTGGCAGTACGGCGAAAAATTTTGCCAGGTATAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGCCCTATTCTGGCGATGAATGGCAGGCATTCttt < 2:359261/143‑1 (MQ=255) aaTCCCAGAAGACCCGCGAGCAGTACGGCGAAAAGTTTCGCCAGGTACAGCAATATCTGCACAGCGGTGATTGCCATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAg < 2:890226/143‑1 (MQ=255) aaTCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAGCGTTTTCATGCGACCTCTTCTGGCGCGGAATGGCAGGCCTTCCTTCAg > 2:676852/1‑143 (MQ=255) gTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCgcgcgc > 1:869/1‑143 (MQ=255) gTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCgcgcgc > 1:313396/1‑143 (MQ=255) tACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCGCGCGcc < 2:191562/143‑1 (MQ=255) tggCGAAATTTTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATggc < 2:221465/103‑1 (MQ=255) tggCGAAATTTTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATggc > 1:221465/2‑104 (MQ=255) tggCGAAATTTTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCGCGCGCCAt > 1:468874/2‑143 (MQ=255) gCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATCCGCGCTTTTTTTGAAGTTTAGGTCCGGCCTTCCTTCAGCTTAATCAGGCCAACCGCGCGCCAttt > 1:779754/1‑143 (MQ=255) aaTTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGTGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCGCGCGCCATTTAGCGCt > 1:784682/1‑143 (MQ=255) | TCATACAGTTTCTTTGCTGAGTCATAATGATGTCAATGCCCGTCGGGCCTGGCTGGAAAGCCAGCAATTCTCGCCGCAGGAAGATTTCACGCTCACTTCCGACTGGCAATCCAATATGACCCGCGAGCAGTACGGCGAAAAATTTCGCCAGGTACAGGAATATCTGCACAGCGGTGATTGCTATCAGGTGAATCTCGCCCAACGTTTTCATGCGACCTATTCTGGCGATGAATGGCAGGCATTCCTTCAGCTTAATCAGGCCAACCGCGCGCCATTTAGCGCT > NC_000913/1895251‑1895533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |