Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,874 T→A 89.1% T481S (ACC→TCC)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,8740TA89.1% 113.4 / 6.5 46T481S (ACC→TCC) zwfNADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base A (14/27);  total (16/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

GCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGT  >  NC_000913/1934734‑1935004
                                                                                                                                            |                                                                                                                                  
gCCTCGGGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGaaa                                                                                                                                  <  1:175903/143‑1 (MQ=255)
     gTGGGTGAATGCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTACAATAACCACAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAatcatc                                                                                                                             <  1:611850/143‑1 (MQ=255)
       gggTGAATCCGCACGAGGCCTGAAAGGGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCAGAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGc                                                                                                                           <  1:1857/143‑1 (MQ=255)
          tGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCAc                                                                                                                        <  2:977599/143‑1 (MQ=255)
             aTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATATGCGCAGATATTACTCAAACTCATTCCAGGATCGATCTTCACATGATATGGTCGCCACCGa                                                                                                                     <  2:461699/143‑1 (MQ=255)
              tCCGCACGGGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGACCACGTTACAGGTAAAATAACCCTACAGGAGAAGCGCCGATATTACTCACACTCATTCCAGGAAGCACCACACCGCGAAATCATCGCCagcaac                                                                                                                    >  2:902409/1‑138 (MQ=255)
               ccGCACGAGGCCTGAAAATGTGCAGATTTTGCTACAATCTGCGCAAAAGCATGTTTACGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAgg                                                                                                                   <  1:954396/143‑1 (MQ=255)
                    cGAGGCCTGAAAGTGTAAAAAGTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACg                                                                                                              <  1:110626/143‑1 (MQ=255)
                          cTGAAAGTGTAAAAATTGTTCGACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTccc                                                                                                        <  2:27766/143‑1 (MQ=255)
                           tGAATGTGTAAATCTTGTTCTACAATCTGCGCAAGATCATGTTACCTGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                       <  2:299121/143‑1 (MQ=255)
                           tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTcccc                                                                                                       <  1:180462/143‑1 (MQ=255)
                             aaaGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAg                                                                                                     >  1:526116/1‑143 (MQ=255)
                             aaaGGGTAAAAATTGTTCTACAATCGGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCAGTCCAGGAATGACCATCACGGGAAATCATCGCCACCGCGGCAACGGGTCCCCAg                                                                                                     <  2:329611/143‑1 (MQ=255)
                                 tgtAAAAATTGTGCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAGGGATAAGCGCAGATATTACTCAAACTCATGCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                                                                                 <  2:1047309/143‑1 (MQ=255)
                                   tacaaaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                               <  1:484206/140‑1 (MQ=255)
                                   tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                               <  2:623830/143‑1 (MQ=255)
                                    agaaaTTGTTCTACCATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                              <  2:850356/141‑1 (MQ=255)
                                    aagcatggTTCTACGATCTGCGCAATCTCATGTTACCGGTAATGTAACCATAAAGCATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                              <  2:741323/136‑1 (MQ=255)
                                    aaaaaTTGTTCTACAATCTGCGCAAGATCATGTTCCCGGTAAACTAACCATAAAGGATAAGCGCGGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                              <  2:864205/143‑1 (MQ=255)
                                             tCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCGACGGGCCCCCGGGTTCCGGCCTGATACg                                                                                     >  1:161116/1‑143 (MQ=255)
                                              cTAGAATCTCCGCGTGATCATGTTATCGGTATAATAACCATAAAGGATAAGCGCAGATATTACCCAAACTCAAGCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCCACGGGTCCCCAGGTTCCGGCCTGATACgg                                                                                    <  2:664493/143‑1 (MQ=255)
                                               tataaTCTGCGCAAGATCATGTTACCGGTAAAATAACCATCAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATACCCACCGAGGGAACGGGGCCCCAGGGTCCGCCCTGacaccgc                                                                                   >  2:1027951/4‑140 (MQ=255)
                                                    tCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGGGTCgg                                                                              >  1:720474/1‑143 (MQ=255)
                                                         gcAAGGTCAGGTTACCGGTATCATAACCATAACGTGTAAGCGCAGATATTACTCAAACTCATTCCAGGTCCGTCCATCACTGTAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcat                                                                         <  1:494227/143‑1 (MQ=255)
                                                          cAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGACCTGATACGGTTTCGGCGcatc                                                                        >  1:996481/1‑143 (MQ=255)
                                                           aaGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatca                                                                       >  1:519795/1‑143 (MQ=255)
                                                           aaGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGCCCCCAGCTTCCGGCCTGATACGGCTTCGgcgccgcc                                                                       >  2:453866/1‑139 (MQ=255)
                                                                                     aTAAAGGATAAGCGCAGATATTACTTAAAGTCATTCCAGGAACGACCTTCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                                             <  1:276751/143‑1 (MQ=255)
                                                                                     aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGACCGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGCCCCCAGGTTCCGCCCTGATACGCGCTCGGCGCATCATTGTCCCTCGCCCACGCCCCAGtcgt                                             >  1:843914/1‑140 (MQ=255)
                                                                                     aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACAGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                                             <  1:747843/143‑1 (MQ=255)
                                                                                     aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTGTCGGCGCATCATTGTCCATCGCGCACGCCTCAGTAAt                                             >  1:843945/1‑143 (MQ=255)
                                                                                     aTAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGGCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt                                             <  1:964592/143‑1 (MQ=255)
                                                                                         aGGATGAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCTTCACGGGAAATCATGGCCACCGAGGCAACGGGTCCCCAGGCTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAg                                         <  2:513734/143‑1 (MQ=255)
                                                                                         aGGATAAGCTCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAg                                         <  2:947758/143‑1 (MQ=255)
                                                                                                       tatTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                           <  2:526116/143‑1 (MQ=255)
                                                                                                          tACTCAAACTCATCCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGACCCCAGGTTCCGGCCTGCCACCGTCTCCCCGCATCACCGCCCATCCCCCCCGCCCCAGTACTGGAGTCCACCCATTCCCAAGc                        >  2:183304/1‑143 (MQ=255)
                                                                                                            cTCACACTCATTCCAGGAACGTCCATCACGGGAAATCATCGCCACCGAGGCAACGGTTCCCCAGGTTCCGGATGAATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGctt                      <  1:959607/143‑1 (MQ=255)
                                                                                                            cTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcgt                      >  1:967385/1‑141 (MQ=255)
                                                                                                             tcgagcTCATTCCAGGACCGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttc                     <  2:196649/138‑1 (MQ=255)
                                                                                                             tCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGAGACCCCAGGTTCCGGCCTGATACGGTTTCGGCCCATCATTGTCCATCGCCCACACATCAGTAATAGCATCTACCCATCACCAAGcttg                     >  2:111132/1‑142 (MQ=255)
                                                                                                                aaCTCCTTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCttcttc                  <  1:993501/143‑1 (MQ=255)
                                                                                                                   tctttCCCGGACCGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCAc               <  2:496363/140‑1 (MQ=255)
                                                                                                                   tCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCATCGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCCTTCTCCATGGGCCACGCCGCAGTAATGCCGTCCACCCATGTCGACGCTTTTTcccc               >  1:637438/1‑141 (MQ=255)
                                                                                                                   tCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCAc               <  2:679673/143‑1 (MQ=255)
                                                                                                                         cAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCCTCATTGTCCATCGCCCACGCCTCAGTCATGGCGTCTACCCATTTCCAGGCTTCTTCCCCTtcgtc         >  1:374879/1‑143 (MQ=255)
                                                                                                                            gAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTTTCCATCGCCCACGCCTCAGTAAGGGAGTCTGCCCATTTCCCGGCTACGTCCACTTCGTcccg      >  1:534792/1‑143 (MQ=255)
                                                                                                                                gACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGt  <  1:858564/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                  
GCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGT  >  NC_000913/1934734‑1935004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑