Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 153.7 / ‑6.7 52I204S (ATC→AGC) wcaAputative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (22/29);  total (23/29)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132648‑2132926
                                                                                                                                           |                                                                                                                                           
gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCt                                                                                                                                          >  1:876873/1‑143 (MQ=255)
 aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCGTGTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                         >  1:285767/1‑143 (MQ=255)
  acTGGTAGAGGGTAAAAAGCGGATATTATTGGCTGGCGCGGTCGAATTTGTCTATGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:424560/142‑1 (MQ=255)
     tgTATAGGGTAAATAGTTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     <  1:10299/142‑1 (MQ=255)
              tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                            >  1:762920/1‑143 (MQ=255)
                   aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                       >  1:621267/1‑143 (MQ=255)
                      tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCTGAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCATGCGCTTCGTCGGCTCTTCTCCTTTCCa                                                                                                                    >  2:379038/1‑143 (MQ=255)
                           ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                               >  1:346474/1‑143 (MQ=255)
                                  cTGTCGTGGGCGACTTTGTCTATGTGTGTTCTGTAAAAGTGGGAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                        <  2:258998/143‑1 (MQ=255)
                                     gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                     >  2:679372/1‑143 (MQ=255)
                                      cgcgGTCGAATTTGGTTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGTATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc                                                                                                    <  2:801166/143‑1 (MQ=255)
                                             gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTCCGCCGTACTccaccacca                                                                                             >  2:940997/1‑143 (MQ=255)
                                             gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCTCTACTTTCCACGGTTCGCCGTACCcccccccca                                                                                             >  1:735141/1‑143 (MQ=255)
                                               aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                           <  1:828684/143‑1 (MQ=255)
                                                 ttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGGTCGCCGGACGCCACACCCAGCCg                                                                                         >  2:827991/1‑143 (MQ=255)
                                                 ttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACATCCg                                                                                         >  1:75308/1‑143 (MQ=255)
                                                      tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  2:896137/1‑143 (MQ=255)
                                                       ttctgCCTGCGGTCAGAGTGGAAGTCCCCGGATAATTGTTTCGGTGACGAGGTGATCTGCCGTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCGCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                   <  2:35480/140‑1 (MQ=255)
                                                        tgtgCTTGCGGTCAAAGTGGAGGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                  <  2:750590/143‑1 (MQ=255)
                                                        tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCATCCGccgccgc                                                                                  >  1:38025/1‑137 (MQ=255)
                                                            cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGACGATgc                                                                              >  2:234548/1‑142 (MQ=255)
                                                             ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                             <  2:346474/143‑1 (MQ=255)
                                                              tGCGGTAAACGTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGACCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                            <  2:332544/143‑1 (MQ=255)
                                                              tGCGGTAAAAGTGGAAGTACCGAGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCCGGAAGATGTCg                                                                            <  2:902779/143‑1 (MQ=255)
                                                               gCGGTAAAAGTGAAACTACTCGGACGTTTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                           <  2:465421/143‑1 (MQ=255)
                                                                 ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTaa                                                                         >  1:305768/1‑143 (MQ=255)
                                                                   tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGCGATCTGCATTTCACCGTGATTGAGCTGCAGGCGCTGCGTCGCCTCGTCTACTTCCCACGGTTCGCCGTCCTCCACCCCCATCCCCAGGAAGACGTcagactc                                                                       >  2:459833/1‑137 (MQ=255)
                                                                       aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                   <  2:484147/143‑1 (MQ=255)
                                                                        gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:155530/143‑1 (MQ=255)
                                                                             aGTACTCGGAGTATTTTTTCGGTGACGAGGTGATTTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                             <  2:838110/143‑1 (MQ=255)
                                                                             aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGACTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                             <  1:19040/143‑1 (MQ=255)
                                                                                    ggAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCTCTTTCAGtt                                                      <  1:646385/143‑1 (MQ=255)
                                                                                    ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGTTCTTCAAGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  1:490636/143‑1 (MQ=255)
                                                                                     gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                     <  1:896137/143‑1 (MQ=255)
                                                                                      agcgtttgttCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCGCGGTTCGCCCTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  2:762920/139‑1 (MQ=255)
                                                                                      agaAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:1037930/143‑1 (MQ=255)
                                                                                          ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                >  1:1017096/1‑143 (MQ=255)
                                                                                            cttacGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                              <  1:234548/139‑1 (MQ=255)
                                                                                              ttCGGTGACGAGTCGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  2:792461/143‑1 (MQ=255)
                                                                                                  gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTATTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  2:824815/143‑1 (MQ=255)
                                                                                                  gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  1:156007/143‑1 (MQ=255)
                                                                                                      tgtggTTATCTGCATTTCACCGTGATTGATTTGCAGGCTCTCCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAATATGTCGTAATCCTGTGCCGCTTTCAGTTCTGTATCGAACAAACAct                                    <  2:614911/140‑1 (MQ=255)
                                                                                                         ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                 <  2:761732/143‑1 (MQ=255)
                                                                                                            caTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                              <  2:710219/142‑1 (MQ=255)
                                                                                                                    tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCAAt                      >  1:211631/1‑143 (MQ=255)
                                                                                                                          cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGACATGCCCAg                >  2:849777/1‑143 (MQ=255)
                                                                                                                           gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCCGTCCGGTATCGCACCACCACCCTTTGACACGCCCAGCCCAgg               >  1:374083/1‑143 (MQ=255)
                                                                                                                            tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTCTCAGTCCGGTATCGACCACACACTCTTTGAAACGCCATGCCCGGGt              >  2:1053393/1‑143 (MQ=255)
                                                                                                                               ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  2:621267/143‑1 (MQ=255)
                                                                                                                                tgatAGGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg          <  1:943996/143‑1 (MQ=255)
                                                                                                                                       cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGGCATGCCCAGGTAAAGACCTGGt   >  1:300697/1‑143 (MQ=255)
                                                                                                                                        aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCAGCTTTCAGTTCGGTATCGAACAAACACGCTTTGAAACGCCGTGCCCAGGTAAAGACCGGGtt  >  1:536346/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132648‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑