Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,323 C→G 9.3% intergenic (+258/+115) yohP → / ← dusC putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,3230CG 9.3% 133.0 / 6.8 54intergenic (+258/+115)yohP/dusCputative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base G (2/3);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAA  >  NC_000913/2229184‑2229457
                                                                                                                                           |                                                                                                                                      
ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGGGGTAACATCCCTGTCAAACCCCCAGTCAGGCCACAACCAtg                                                                                                                                     >  2:228223/1‑143 (MQ=11)
    aaTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                                                                                                                                 <  1:991351/143‑1 (MQ=32)
    aaTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACATCCCCGTACAACCCGCCACCAGCCCACATCCAtccggg                                                                                                                                 >  2:458657/1‑138 (MQ=11)
     accgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATGGATGTGGGc                                                                                                                                <  1:750855/141‑1 (MQ=12)
     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCCTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGc                                                                                                                                <  2:293886/143‑1 (MQ=17)
     aTCGCCGCCGCCCCCTCCACCCCCGGGCTCTGGCGCACAATATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCCTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCATGTGGGc                                                                                                                                <  2:468611/143‑1 (MQ=11)
          cgccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATGGATGTGGGCTgagg                                                                                                                           <  1:49127/143‑4 (MQ=12)
              gccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc                                                                                                                       <  2:785826/143‑1 (MQ=32)
                   cTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTACGGTCCCCGCAAACCGCGCCCTCAGCCCACCTCCATGTGGGCTGCCCCGGCCTTCa                                                                                                                  >  2:1043813/1‑143 (MQ=11)
                     gcgccgccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAgg                                                                                                                <  1:497708/137‑1 (MQ=17)
                              ggCTCTGGCGAACAGAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTc                                                                                                       <  1:517862/143‑1 (MQ=17)
                               gCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCg                                                                                                      <  2:501856/143‑1 (MQ=32)
                                     gCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCCTGTGGGCTGCCCCGGCCCTCCCGCACCGCCCCGGCCAtt                                                                                                >  2:861671/1‑143 (MQ=11)
                                     gCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCCGGCAACGCCTCGGCACtt                                                                                                >  1:797844/1‑143 (MQ=16)
                                     gCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAAtt                                                                                                >  1:582507/1‑143 (MQ=35)
                                     ccGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGCAGGGCGCCAGGTGCCATGCCTGTAAAACGCGCCACCAGCCCACACGCATGTGGGCAGCCCCGCCCTCCAGGGAACGCCTCGCCCAtt                                                                                                >  2:1024991/2‑143 (MQ=11)
                                          cAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACCTCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGCACGCCACGGCaaccttgcc                                                                                           >  1:280435/1‑136 (MQ=11)
                                               tCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGccac                                                                                      <  1:414173/143‑1 (MQ=35)
                                                  ccgcTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGACGGGCGCGAGGGAGCATCCCTGTGACACGCGCCCTCAGCCCGCATCCATGTGGGCTGCCGCGGCCTTCAGGGTACGCCGCGGCCATGTTGGCGCCAGCaa                                                                                   >  1:20892/1‑143 (MQ=11)
                                                             tGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCg                                                                        <  2:98463/143‑1 (MQ=255)
                                                             ccctcttggCTCATCCCCTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCTATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCg                                                                        <  1:338509/136‑1 (MQ=14)
                                                                 cTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTCCAGGGAACGCCTCGGCCATTTTCACGCCACCACACACCCGTGCGCCCt                                                                    >  2:954317/1‑143 (MQ=14)
                                                                  tCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTa                                                                   >  1:728651/1‑143 (MQ=255)
                                                                           cccTTGCGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAag                                                          <  2:59000/143‑1 (MQ=255)
                                                                            ccTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAaga                                                         <  1:754764/143‑1 (MQ=255)
                                                                             cTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACCACCGTGCGGCCTATTGATAAagag                                                        >  2:621565/1‑143 (MQ=255)
                                                                                     tACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTCTTGATACAGCGCTACcaca                                                >  1:85085/1‑143 (MQ=255)
                                                                                          ggTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTc                                           <  1:485630/143‑1 (MQ=255)
                                                                                            tCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaa                                         <  2:892794/143‑1 (MQ=255)
                                                                                                gcgcGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaaaa                                     <  1:645906/143‑1 (MQ=255)
                                                                                                  gcgAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACa                                   >  1:558329/1‑143 (MQ=255)
                                                                                                   cgAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACAt                                  <  1:108847/143‑1 (MQ=255)
                                                                                                   cgAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACAt                                  <  2:599842/143‑1 (MQ=255)
                                                                                                            aTCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGt                         >  1:1068258/1‑143 (MQ=255)
                                                                                                              tccTGTAAAACGCGCCCTCAGCCCACATTCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttt                       <  2:269887/142‑1 (MQ=255)
                                                                                                              gcgggTAAAACGGGCCGTCGGCCGGCATCGATGTGGGCTGCGCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAATCGTGCCGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttt                       <  2:1042315/139‑1 (MQ=25)
                                                                                                              cccTGTAAAACGGGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttt                       <  2:785802/143‑1 (MQ=255)
                                                                                                              cccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttt                       <  2:901025/143‑1 (MQ=255)
                                                                                                              cccGGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttt                       <  2:820059/143‑1 (MQ=255)
                                                                                                               ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTTCCCCAGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttt                      <  1:445044/143‑1 (MQ=255)
                                                                                                               ccTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttt                      >  1:392813/1‑143 (MQ=255)
                                                                                                                ctgcagaaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttttt                     <  2:638902/137‑1 (MQ=255)
                                                                                                                ccgTAAAACGCGCCCTCAGCCCACATGCATGTGGGCTGCCGCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATACAGAGCTAACACATTGTCAAAAAACATCACTATGGttttt                     <  1:61510/141‑1 (MQ=255)
                                                                                                                cTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGttttt                     >  1:265807/1‑143 (MQ=255)
                                                                                                                 tGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGtttttt                    <  1:694037/143‑1 (MQ=255)
                                                                                                                 tGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGACAAAGAGATAACACATTGTCAAAAAACATCACTATAGtttact                    >  2:353033/1‑140 (MQ=255)
                                                                                                                 tGTAAAACGCGCCCTCAGCCCACATCCATGTGGGATGTTCCGGCCTGCAGGGAAAGCCTCGGCAATTTTGCCGCCACCAAACAACCGAGCGGCCTATTGATAAAGAGCTAACACATGGTCAAAAAACGTCACTATGGtttgtt                    >  2:478095/1‑143 (MQ=21)
                                                                                                                     aaaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTGTGTagag                >  1:321255/1‑143 (MQ=255)
                                                                                                                     aaaCGCGCCCTCAGCCCACATCCATGTGGACTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACCCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACGATGGCTCTTTagag                >  1:672639/1‑143 (MQ=25)
                                                                                                                      aaCGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGTCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGt               >  1:200752/1‑143 (MQ=255)
                                                                                                                             ccTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGa        <  1:211802/143‑1 (MQ=255)
                                                                                                                              cTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCCCGGCAATTACAAAGTCTATGAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGat       >  1:836516/1‑143 (MQ=25)
                                                                                                                                   cccACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCaa  >  1:33371/1‑143 (MQ=255)
                                                                                                                                   cccACATGGATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGAGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTATCGATATCaa  >  1:857397/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                      
GGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAAAACATCACTATGGTTTTTTAGAGTTTCTCGATATCAA  >  NC_000913/2229184‑2229457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑