| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 349,717 | T→C | 13.4% | intergenic (+145/‑295) | prpB → / → prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 349,717 | 0 | T | C | 13.4% | 96.0 / 3.0 | 60 | intergenic (+145/‑295) | prpB/prpC | 2‑methylisocitrate lyase/2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base T (27/19); new base C (5/4); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACG > NC_000913/349577‑349858 | cGCCCGTTGATGGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa < 2:620922/143‑1 (MQ=255) gCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGAc > 1:531010/1‑143 (MQ=255) ccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGGCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACtt < 2:311564/143‑1 (MQ=255) ccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGCTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACtt > 1:540096/1‑143 (MQ=255) gTTGATTACATACGCGAGCCGACGCCTGATGCGTCAATGACGCGACTTATAACGCCTCCGGGGTGCTCTGACGTTTAGGTCGGTTAAGACGGCTGGCGACGCGTCCCACAACCGATGCCTGATGCGCCGCTGACGTGACTTAt < 2:271300/143‑1 (MQ=9) tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGCACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGACGCCCGGAGCGCCGCCGCCGCGACTCAGCAGcccgc > 2:240049/1‑141 (MQ=14) tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGGGGCGTAGCAGGCCTa > 2:633380/1‑143 (MQ=14) aTTCGACAGCCGATGCCTGATGCGTCGCTCACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGCCAACCGATCCCCCATGCGCCGCTCACCTCCCTTACCCGGccgcc > 1:1037093/1‑140 (MQ=14) ttCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGCCCTACGAGGTGCACGGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGCTGCCTGATGCCCCGCTGACGTCACCTGTCAGGccgccg > 1:368046/1‑139 (MQ=14) tCGCCAGCCGAGGCCTGATGCGTCGCTGACGCGACTTATCCGGCCTACGAGGGGCACTGAGCTGTAGGGTGGCTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACgg < 2:266687/143‑1 (MQ=14) cGACAGCCGATGCCTGATGCTTCTCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTTGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 1:122705/143‑1 (MQ=255) cGACAGCCGATGCCTGATGCGTCGCTGGCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg < 2:1073768/143‑1 (MQ=255) cAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTg < 1:1014052/143‑1 (MQ=255) ccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCAc > 1:934107/1‑143 (MQ=255) ccGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCACCGACGCGAATGATCAGGCCTCCGGCGTGcga > 2:351689/1‑141 (MQ=11) cGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGTATGGCGTCGCATCCGACAACAGTTGCCTGATGCGCCGCTGACGTGACTGATCAGGCCTAGgggggacaca > 1:75201/1‑137 (MQ=14) cGATGCCTGATGCGTCGCTGACGCGACATATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACt > 2:651921/1‑143 (MQ=255) tgacggATGCGTCGCTGACGCGACTGATCAGGCCTCTGGGGCGCACTGAACTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa < 2:470828/138‑1 (MQ=14) taccTGATGCGTCGCTGACGCTACTTACCAGGCCTACGACGTGCACTGAACTGGAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGTTGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa < 1:256022/141‑1 (MQ=14) tGCCTGATGCGTCGCTCCCGCGACTTATCAGGCCTACGGGGTGCTCTGAACTGTAGGTCGGATAAGACGGATGTCGTCGCATCCGACAACCGATGCCTGATGCGCCACTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa < 2:878006/143‑1 (MQ=11) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGAgcactgcact > 1:121507/1‑143 (MQ=255) ccTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCCGGCCTACGGGGCGCCCCGAACt > 1:762255/1‑143 (MQ=14) tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCAATGAACTgc > 1:937842/1‑142 (MQ=255) aTGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGGGCACTCGACTGtcg > 1:165889/1‑141 (MQ=255) tCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg > 1:480274/1‑143 (MQ=255) gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTCGGTCGGATAAGGCg > 1:99450/1‑143 (MQ=255) ggCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCAATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCCCTGAACTGTAGGTCGGCTAACACCCACAGCGTCGc > 1:248201/1‑143 (MQ=14) gCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTGGGATAAGACGCACAGCGTCGCa > 1:419883/1‑143 (MQ=255) gCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACAGTAGGTCGGATAAGACGCATAGCGTCGCa > 1:538605/1‑143 (MQ=255) tACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATCGCGTCGCATcc > 1:124727/1‑143 (MQ=255) cGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa > 2:595054/1‑143 (MQ=25) aGGTGCACTGAGCTGGAGGGCGGAGAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACa < 2:909206/143‑1 (MQ=255) gCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCg < 2:1035490/143‑1 (MQ=14) gtggagcTGTAGGGCGGATAAGCCGGATGGCGTCGTATCCGACAGCCGATGCCTGATGCGCCGCTGACGCAGCTTATGAGGCCTACGGGGTTCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGAt < 2:377223/137‑1 (MQ=11) tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGc > 1:1050160/1‑143 (MQ=38) aaCTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt < 2:566198/143‑1 (MQ=17) aCTGTAGGTCGGATAAGACGGAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTg < 2:47246/143‑1 (MQ=37) tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGAt > 1:171547/1‑143 (MQ=38) aGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTAGCAGGGATAAGGGGTGCGCTGAGATGTAGGGCGGGTGAGAGGGATGGCGTCGGATCCGACAGCCGATGCCTGATgcg > 2:177386/1‑143 (MQ=14) gTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGt > 2:330043/1‑79 (MQ=255) gATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg < 1:628888/143‑1 (MQ=37) gACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCGTCCGACAACCGATGCCTGGTGCGCCGCTGAcgcg > 1:75283/1‑143 (MQ=255) tGGCGTCACATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACGGAACTGGAGGTCGGATAAGACGCGTAGCGGCGCATCCCACAACCGATGCCTAAAACACCGCGGACGCGAcagat > 2:958274/1‑139 (MQ=11) gcatCGCACGCGACAACAGATGCCTGATGCGCCGCTGCCGTGACTTCTCACGCCACCGGTGTGCACTGGACTGTGGGTAGGATAAGCCGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa < 1:17537/140‑1 (MQ=11) cGTCGCGTCCGCCAACCGATGCCTGATGCGCCGCTGACGTGACTTATCGGGCCTACGGGGTGCACTGAACTGTAGTTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAg < 2:476760/143‑1 (MQ=255) cGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTAAAGCGCCGCAGACGCGCCTTATCAGGCCTACggg > 2:574854/1‑143 (MQ=255) gACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAGATGCGTGATGCGCCGCTGACGCGAGTTATGAGGCCTAAgggg > 1:748203/1‑143 (MQ=11) gACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGCATAAGACGCATACCGTCGCATCCGCCAACCGCTACCAGCAGCGCGACTGACGCGACCTCTCAGACCAAcaagg > 2:872183/1‑139 (MQ=11) cAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTg < 1:651921/143‑1 (MQ=32) aCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCa < 1:726944/143‑1 (MQ=33) tGCGTGATGCGCCGCTGACGGGACTTATCAGGCCTACGGGGTGCACTGACCTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa < 2:236530/143‑1 (MQ=12) tGCCTGATGCGCCGCTGACGTGACTTATCAGGCCCACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa < 1:302638/143‑1 (MQ=25) tGCCTGATGCGCCGACACAGGGACTTAGCGGGCCTACTGTGTGCACTGCACGGTAGGTCGGATAAGACGCATAGCGGCGCCTCCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGaa < 2:648002/143‑1 (MQ=11) gCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGCGCACTGAAc < 1:924517/143‑1 (MQ=25) cTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGACCTg > 1:1055108/1‑143 (MQ=25) gcgcCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa > 2:3545/1‑59 (MQ=38) tccgcTGGCGGGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg < 2:484411/142‑1 (MQ=17) cgcttgcGTGACTTAGCAGGCCTACGGGGTGCACTGAACTGTAGGTGGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGa < 1:142554/137‑1 (MQ=255) gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACg > 2:809666/1‑143 (MQ=33) gTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGAAAACCGATGCCTGATGAACCGCTGAAGCGACTTATCAGGCATAGGGGGAGCACTCAACAGTAGGACGGATAAGACg > 2:671966/1‑143 (MQ=11) | CGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACG > NC_000913/349577‑349858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |