| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,910,100 | C→T | 12.1% | D196N (GAC→AAC) | mazG ← | nucleoside triphosphate pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,910,100 | 0 | C | T | 12.1% | 111.8 / 5.8 | 52 | D196N (GAC→AAC) | mazG | nucleoside triphosphate pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (20/24); new base T (3/3); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
ATTTCCAGTCCACGCGCGGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCT > NC_000913/2909964‑2910240 | aTTTCCAGTCCACGCGCGGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCAt > 1:1033603/1‑143 (MQ=255) agcccgcgcgcgGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTtcctc < 2:775082/137‑1 (MQ=255) gcgcgGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTGTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGt < 2:295894/143‑1 (MQ=255) cgGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTGCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTa < 1:561781/143‑1 (MQ=255) gcagcaCTACGCTCCACTTCGCGAAAACGACGTTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTGAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGc < 2:229796/143‑1 (MQ=255) gCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTGTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGc < 2:580035/143‑1 (MQ=255) aaTACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCATACCTATTTGGCGAGCCAGATTACAGGTGGCAAACAGCAAACAACCCATTGCTTCCTCCAGTTTAGCCTGGt > 1:488075/1‑143 (MQ=255) aTAGGCTCCTCTTCTCGAAAGCGACGCTCGAATTTTTCGTTCGCTTTTTGGAATGCGATTTCTGTTTTCGTCCTTAAATGGCGTGCCAGATTAACCGCGGCAAACAGCAGGTCTCCCATTTCCTCCTCCAGTTTAGCCTGGTc < 2:406532/143‑1 (MQ=255) tACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACGGCAGGTCCCCCATTTCCTCCTCCAGTTCAGCCTGGTCg > 2:637614/1‑143 (MQ=255) tCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGacaac > 1:440639/1‑143 (MQ=255) cACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAg < 2:669533/143‑1 (MQ=255) ttCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAGTGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCCTTTCCTCCTCCCGTTCACCATGGTCGACGACGGCCt > 2:702693/1‑143 (MQ=255) cgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGCGCGACAACAGCCTGCcg > 2:433390/1‑143 (MQ=255) cgacgCTCGAATTTTGCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTc < 2:924927/143‑1 (MQ=255) tCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACAt < 1:349882/143‑1 (MQ=255) gtttCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTTCCTAGATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCtc < 2:881015/142‑1 (MQ=255) ttcgCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGTGAGCCAGATTAACCGTGGCAAACAGCAGGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACAACACCTCGTCGAt > 2:355046/1‑143 (MQ=255) ttcgCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 2:355047/1‑143 (MQ=255) ttcgCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt > 2:355040/1‑143 (MQ=255) cTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTTGCAAACAGCCGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACACCAGCCCGCCGCGCACCGTCCATCCCCTCGTCGCtccgt > 2:930409/1‑140 (MQ=255) cAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATCTCCTCCTCCAGTTTCGCCTGGTCCACAACAGCCTGCCCCGCTTCGTCCATCACCTCCTCGACCTCTTCGCAGa > 2:666955/1‑143 (MQ=255) tGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCATCGATCTATTCGAAGACtt > 1:804440/1‑143 (MQ=255) gATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTAt < 1:376398/143‑1 (MQ=255) gATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTAt < 1:645738/143‑1 (MQ=255) gATTGCTGCTTGCGTCCCTGAATGGCGAGCCAGATCAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTAt < 1:681846/143‑1 (MQ=255) aaTTCCTGCGTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCCTAGACTTTAt < 2:1053047/142‑1 (MQ=255) cGTCCCGAAAGGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc < 2:702789/143‑1 (MQ=255) ccTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGACTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTGCGTACACGTAATCGACTACCGGACCAAg > 2:412391/1‑143 (MQ=255) cTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGACTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCACCTACCGAAcccacc > 2:321777/1‑139 (MQ=255) cTAAATGGCGAGCCAGATTAACCGGGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGCTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGc < 1:39383/143‑1 (MQ=255) tAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCCAGcg > 2:125923/1‑143 (MQ=255) tAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcg > 1:898718/1‑143 (MQ=255) tGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcgt > 1:991354/1‑143 (MQ=255) cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTGGATCTCTTCGTAGACTTTATCGAGTACCGGACGAAGCGTCGTccg > 1:630285/1‑142 (MQ=255) aGATTAATCTTGGCAAACAGCAGGTGCCTCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGaa < 1:501862/143‑1 (MQ=255) gATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAg < 2:787414/143‑1 (MQ=255) acggTGGCTATCAGCAGGTCCCCCATTTCCTCCTCGAGTTTTGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGTTCCTATTGTCGTCCAATGGAAGCCAAc < 2:154278/140‑1 (MQ=255) cccAAATAGCAGGCCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTgg < 2:1033603/141‑1 (MQ=255) cAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCACCGTTGgcg > 1:878095/1‑143 (MQ=255) aaaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCACTCGAAGCCAACCTTgaccc > 1:979636/1‑139 (MQ=255) aaaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCACCGTTGgcgc > 1:1032792/1‑143 (MQ=255) aaaCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTATATCGACTACCGGACCAAGCGGCGTCCAATCGAAGACAACGCTGacgc > 2:587886/1‑143 (MQ=255) cagtagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAAc < 1:220361/143‑1 (MQ=255) agcagGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTTGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACg < 1:355040/143‑1 (MQ=255) agcagGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACg < 1:355047/143‑1 (MQ=255) agcagGTTCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACg < 1:355046/143‑1 (MQ=255) agcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACg > 1:201585/1‑143 (MQ=255) gcagGTCCCCCATGTCCGCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGt < 2:32813/143‑1 (MQ=255) agGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGttt < 1:250280/143‑1 (MQ=255) ggTCCCCCAGTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCATCGCTCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTc < 2:1054532/143‑1 (MQ=255) gTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAAGCGAAGCCAACGTTGGCGCACCGCTTcc > 1:437586/1‑142 (MQ=255) gTCCCCCATTGCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCt < 2:48200/143‑1 (MQ=255) | ATTTCCAGTCCACGCGCGGCAACAATACGCTCCACTTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCT > NC_000913/2909964‑2910240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |