| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 100.0% | 107.9 / ‑6.7 | 38 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (20/17); minor base G (1/0); total (21/17) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041501‑3041773 | cGACGCCAAAGGTAAACTGTGGAGCAATTTACGTCTGTTCCGCGACGCCCATCACTATGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCgcgc < 1:608121/143‑1 (MQ=255) aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTGTAAAGTGGCCATGGCGGCAgacgc > 2:884529/1‑142 (MQ=255) gTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAg > 1:461590/1‑143 (MQ=255) aaaaTGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCg < 1:298520/143‑1 (MQ=255) gcTGTTCGGCGTCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg < 2:415618/142‑1 (MQ=255) tGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGt < 2:490774/143‑1 (MQ=255) ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGttt > 1:691219/1‑143 (MQ=255) ttCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCGGttt > 1:889791/1‑143 (MQ=255) tCCGCGAGGGCGATGGCTTGGCATGGACTGAACGGCGCAGCGGGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtttt < 1:363465/143‑1 (MQ=255) cgcgGCGGCGAGGGCTTGGCACGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCa < 2:1047505/143‑1 (MQ=255) cTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCCGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgccgcgc < 1:425620/143‑1 (MQ=255) tGCATGGATTGACCTGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAACATATGCGGTATTCTCTACAGTGCCCATCGCGCTAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg < 2:89542/143‑1 (MQ=255) aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGGGCTGCTTGGAGTTGCCGGATCACAAGCGCGGCCCGCGCTGGCaa > 2:50147/1‑143 (MQ=255) tGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCGGCGCTGGcgag > 2:889895/1‑140 (MQ=255) ggATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAt < 2:821363/143‑1 (MQ=255) aCGGGGTAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa < 2:489406/143‑1 (MQ=255) aCGGCGCAGCGTGCGTGAGCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa < 1:589282/143‑1 (MQ=255) gtgcgcAGCTGGCGTGAACCGCCGGTGTCGGAACTGAAAAAACATGCGGTATTCTCTAAGGTGCCCATCGCGCCAGACGACGGGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAg < 2:606742/141‑1 (MQ=255) ggCGCAGCGTGCGTGAACCGCAGCTGACTGAACGGCTATATGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTCTCAGGCGCGCGCCGCGCTGGCAAATCCCTTTAGc > 2:878784/1‑143 (MQ=255) tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTCGCCGGGTTTCAGGCGCGCGCCGCGCTCGCACATCTCTTTAGCGAACTGCCt > 2:549359/1‑143 (MQ=255) tgcgtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCCTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCGGGCAAATCTCCTTAGCGAAcgggct > 2:550222/1‑138 (MQ=255) gtgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGACCTGCTTTCg > 1:191732/1‑143 (MQ=255) aaCCGCAGCTGACTGAACTGAAAAAATATGCGTTATTCTCTAATGTGCCCATCGCGCTAGACGACGAGAGTGTGCTGCATGGTGATGCAGGTTCTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaa < 1:44339/143‑1 (MQ=255) aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag < 1:263222/143‑1 (MQ=255) ccGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCGAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTCCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTTgaaagg > 2:1050999/1‑142 (MQ=255) aGCTGACTGCACTGAAAAAATATGCGGTATTGTCTACTGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGaaaaa < 2:221021/143‑1 (MQ=255) gCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTCTAGCGAACTGCCGTCGCAAGCAAGAc > 2:686083/1‑143 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAACTCTCTTTAGCGAACTGCCTTCGAAAGAAACACAGGTAg > 2:612569/1‑143 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGACAGACGACGAGCGTGTGCTGCTTGGTGTTGACGGCTTTCAGGCGCGCGCCGCGCTGGCAACTCTCTTCAGCGAACTGCCTTCGAGAGAAAACCAGCTAg > 2:31673/1‑143 (MQ=255) aactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACAGAAAAACAGGCAGTc > 2:44506/1‑143 (MQ=255) gaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGCTGCCGGTTGTCAGGCGCGCGCCGCGCTGGCCAATCTCTTCCAAGCACGGCCTGCGAACGAAAAACAGGCAGCCGaag > 2:9992/1‑143 (MQ=255) tGCGGTCTTCCCTCAAGCGCACAGCTCGCCCCACGACTAGCGTGTGCTGCTTGATGTCGCCGGATTTCAGGCGCGCGGCGCGCTGGCAAATATCTTTAGCGAACTGCCTTCGAAAGAACAGCAGGTAGTCAAAGAAGGCGCGa < 2:940556/143‑1 (MQ=255) tGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAGAGAAAAACAGGGAGTCACAGAAGGcgcgc > 1:570855/1‑142 (MQ=255) gCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCCTCGAAAGGAAAACAGGTAGTCAAAGACGGCGCGAc > 2:237495/1‑143 (MQ=255) ggTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATATCTTTAGAGAACTGCCTTCAACAGAGGTACAGGTAGTCAAAGAAGGCGCGACCa > 2:512709/1‑143 (MQ=255) tctcTAAAGTGCCCTTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGGTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTg < 1:44342/143‑1 (MQ=255) tctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCACATCTCTCTAGCGAACTGCCTTCGAAAGAAACCCCGGTAGCCACAGAAGGCGCGCCCCCGTTGcc > 2:392575/1‑142 (MQ=255) gTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGtt > 1:873298/1‑143 (MQ=255) | CGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041501‑3041773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |