Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,143,816 G→T 6.3% P117T (CCG→ACG)  hybB ← hydrogenase 2 membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,143,8160GT 6.3% 174.7 / 2.5 65P117T (CCG→ACG) hybBhydrogenase 2 membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base T (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

GTAGCGACACCTTCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAG  >  NC_000913/3143675‑3143958
                                                                                                                                             |                                                                                                                                              
gTAGCGACACCTTCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCAATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGc                                                                                                                                               >  1:926876/1‑143 (MQ=255)
   gCGACACCTTCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGGCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAgg                                                                                                                                            <  1:526299/143‑1 (MQ=255)
    cGACACCTTCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCCAGTCCACGTTGAAGTGACCCGGCATGTGGAAGTACGGCAGGt                                                                                                                                           >  1:361982/1‑143 (MQ=255)
     gccacCTTCCACCCCAGCCGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGtt                                                                                                                                          <  1:76182/141‑1 (MQ=255)
       caacTTCAACCCCAGACGATCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTcc                                                                                                                                        <  1:128269/140‑1 (MQ=255)
           ttCCCCCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTa                                                                                                                                    <  2:1032832/143‑1 (MQ=255)
           ttCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAATACCGCAGGTTCCAGTa                                                                                                                                    >  2:4057/1‑143 (MQ=255)
           ttCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGGTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTa                                                                                                                                    >  2:387658/1‑143 (MQ=255)
            tCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAg                                                                                                                                   <  1:881575/143‑1 (MQ=255)
                ccccAGACGCTCAAACAGTCCCTGAGCAACCTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGAc                                                                                                                               <  1:23511/143‑1 (MQ=255)
                   cAGACGTTCACACAGTGCCGGAGCAAACTCCAGTGCCACCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCa                                                                                                                            <  1:32322/143‑1 (MQ=255)
                   cAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGCCCGCCGTGTCGAACAGTACCGAGCTCCCGTGGCAGACCCCCGGCACGTCGAAGAACGGCAGGATCCGCTAGCGAcccc                                                                                                                            >  2:645950/1‑142 (MQ=255)
                        gTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCg                                                                                                                       >  1:572222/1‑143 (MQ=255)
                         ttCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGCATGTAGAAGTACGGCAGGCTCCAGTAGCGACCCACGCCGa                                                                                                                      >  2:908049/1‑143 (MQ=255)
                         ttCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGGCCCCCGTCgg                                                                                                                      >  1:754488/1‑142 (MQ=255)
                           cAAGCAGGGCCGGCGCAACCACCAGTGCCATCCCGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATa                                                                                                                    <  2:643678/143‑1 (MQ=255)
                             aaCAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCCATAGt                                                                                                                  >  2:341116/1‑143 (MQ=255)
                                 gagCCGGAGCAAACTCCAGTGCCATCACGCCGAGATAGAGGGTCAGACACACCGCCGTCTCGAACTTTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATc                                                                                                              <  1:955563/141‑1 (MQ=255)
                                      ggAGCAACCTCCAGTGCCAGCACGCCGGTATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACaa                                                                                                         <  2:1011098/143‑1 (MQ=255)
                                      gcatcAACCTCCAGTGCCATCACGCCGATAGCAATGTTCATACAGACCGCCGTCTCGAACAGCACCGAGTTCACGTCGAAGTGACCCGGAATGTAGAAGTCCGGAAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACaa                                                                                                         <  2:1051392/139‑1 (MQ=255)
                                        aGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACGGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGc                                                                                                       <  2:46725/143‑1 (MQ=255)
                                           aaatgctaGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCAc                                                                                                    <  2:353267/136‑1 (MQ=255)
                                              cTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCa                                                                                                 <  1:992352/143‑1 (MQ=255)
                                               tCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGCCCCGGAATGTAGAAGTACGCCAGGTTCCAGTAGCGACCCACGTCGATAGTGATAGACACGCCACCCAg                                                                                                >  2:403815/1‑143 (MQ=255)
                                                  aGTGTCCTCACGCCGATATAGAGGGTCATACAGCCCGCCGTCTCGAACAGTACCGCGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGa                                                                                             <  2:785932/143‑1 (MQ=255)
                                                  aGTGCCATCACGCCGAAAGAGGTGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGGTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGa                                                                                             <  2:1048757/143‑1 (MQ=255)
                                                      ccATCACTGCGATATAGGTGGTCATACCGACGGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTaa                                                                                         <  2:944438/143‑1 (MQ=255)
                                                       cATCACCCCGACATAGAGGGTAATACAGACCACCTTCTCCAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAc                                                                                        <  1:285563/143‑1 (MQ=255)
                                                         tCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAcga                                                                                      >  1:969278/1‑141 (MQ=255)
                                                           aCGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGCTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGCGAGTAACCaga                                                                                    >  2:697311/1‑141 (MQ=255)
                                                           aCGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGGTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCaaa                                                                                    >  1:901670/1‑143 (MQ=255)
                                                               cGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGCTCGACAAGCCACCCAGTGAGTAACCAAACAGa                                                                                >  1:185860/1‑143 (MQ=255)
                                                                  tataGATGGTCATCCAGACCGCCGTCTCGAACAGTACCGCGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTc                                                                             <  1:610875/143‑1 (MQ=255)
                                                                  tataGATGGTCATACAGCCCGCCGTCTCGCACAGGAACGAGTTCACGTTGAAGGGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTc                                                                             <  1:318619/143‑1 (MQ=255)
                                                                         ggTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACa                                                                      <  2:926876/143‑1 (MQ=255)
                                                                             aTACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAgcgc                                                                  >  1:909148/1‑143 (MQ=255)
                                                                              gaCAGACCGCCGACTCGAACAGTACCGAGGTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGcc                                                                 <  1:600835/142‑1 (MQ=255)
                                                                                  gACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGTCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAc                                                                                        <  1:305022/116‑1 (MQ=255)
                                                                                  gACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGTCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAc                                                                                        <  1:305475/116‑1 (MQ=255)
                                                                                  gACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGTCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAc                                                                                        >  2:305022/1‑116 (MQ=255)
                                                                                  gACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGTCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAAc                                                                                        >  2:305475/1‑116 (MQ=255)
                                                                                                  aGTACCGAGTTCACGTTGAAGTGGCCCGGAATGGAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTa                                             <  2:927438/143‑1 (MQ=255)
                                                                                                      ccGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGc                                         >  1:322355/1‑143 (MQ=255)
                                                                                                       cGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCGGTGAGTCACCGAACAGTCTCCCCAACTGCCCCGGACGCACCACCCGCTGGTATTAcc                                        >  1:415074/1‑143 (MQ=255)
                                                                                                           ttCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTAATGCCCACg                                    >  1:923699/1‑143 (MQ=255)
                                                                                                            tCACGGTGGAGTGACCCGGAATGTAGAAGTACGGCAGGTCCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCATTGAGTAACCATACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACgg                                   <  2:73125/143‑1 (MQ=255)
                                                                                                              aCGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGtt                                 <  1:465247/143‑1 (MQ=255)
                                                                                                              aCGGTGAAGTGACCCGGAATGCAGAATGACGGCAGGTCCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGtt                                 <  2:423972/143‑1 (MQ=255)
                                                                                                                 ttGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTaaa                              <  2:782832/143‑1 (MQ=255)
                                                                                                                    aaGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGAc                           >  1:579077/1‑143 (MQ=255)
                                                                                                                     aGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGACGGTATCGCCCACAGTTAAAGACg                          >  2:921184/1‑143 (MQ=255)
                                                                                                                       tGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGta                        >  2:517514/1‑143 (MQ=255)
                                                                                                                       tGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGCATTGCCCACGGTTAAAGACGta                        >  1:647971/1‑143 (MQ=255)
                                                                                                                         aCCCGGCATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATCGTGATCGACAAGCCACCCAGTGAGTAACCAACCCGACTCGCCACCAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGtata                      >  2:221536/1‑143 (MQ=255)
                                                                                                                            cGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCg                   >  1:708824/1‑143 (MQ=255)
                                                                                                                              gAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGCTGGTATTGCCCACGGTTAACGACGTCTACAGcc                 >  1:531665/1‑143 (MQ=255)
                                                                                                                              gAATGAAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGcc                 <  1:748402/143‑1 (MQ=255)
                                                                                                                                 tGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGCATTGCCCACGGTTCAAGACGTATAcccccccc              >  2:672196/1‑141 (MQ=255)
                                                                                                                                 tGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCAATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGCTGGTATTGCCCACGGTTCAACACGTCTACCGCCCAc              >  2:1059078/1‑143 (MQ=255)
                                                                                                                                   tAGAGGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGc            <  1:13047/143‑1 (MQ=255)
                                                                                                                                   tAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGc            <  2:337841/143‑1 (MQ=255)
                                                                                                                                   tAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGc            <  1:962596/143‑1 (MQ=255)
                                                                                                                                    aGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCCCCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGcc           >  1:89431/1‑143 (MQ=255)
                                                                                                                                     gAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCGGCGGATGGTATTGCCCACGGTTAAAGCCGTATACCGCCCACGCCa          >  1:280202/1‑143 (MQ=255)
                                                                                                                                     gAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCa          <  2:47826/143‑1 (MQ=255)
                                                                                                                                            ggCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCa   >  2:342514/1‑143 (MQ=255)
                                                                                                                                             gCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAAGAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCGAg  >  1:794413/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GTAGCGACACCTTCCACCCCAGACGTTCAAACAGTGCCGGAGCAAACTCCAGTGCCATCACGCCGATATAGATGGTCATACAGACCGCCGTCTCGAACAGTACCGAGTTCACGTTGAAGTGACCCGGAATGTAGAAGTACGGCAGGTTCCAGTAGCGACCCACGTCGATAGTGATCGACAAGCCACCCAGTGAGTAACCAAACAGACTCGCCAACAGCGCCGGACGCACCAGCGGATGGTATTGCCCACGGTTAAAGACGTATACCGCCCACGCCAGCGCCCAG  >  NC_000913/3143675‑3143958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑