| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,711,031 | G→A | 100% | P326L (CCG→CTG) | yhjX ← | putative pyruvate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,711,031 | 0 | G | A | 93.5% | 150.0 / 0.3 | 57 | P326L (CCG→CTG) | yhjX | putative pyruvate transporter |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (22/31); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACGTAGAAGGTCACATAGAAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGAT > NC_000913/3710892‑3711170 | cACGTACGAGGTGACATAGAAGCCGCCGAACAGTGAGGCGATCATCTAACCACAAATGCTACCGAGACCGAAACCGAGATAAATCACACCGGAGTGTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGaa < 2:1004966/143‑1 (MQ=255) aCGTAGAAGGTCACATAGAAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGaaa < 1:804322/143‑1 (MQ=255) gtggAAGGTCCGATGGCAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGa < 2:778039/140‑1 (MQ=255) gTAGAAGGTCACATAGAAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACCCCGTCGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGa > 1:124532/1‑143 (MQ=255) gTCACATAGAAGCCGCCAAACAGTCAGGCCATAATCCAACCACAAACGCTCCCGATACCGAACCCGAGATAAATCACACCGTAGTCTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAAt < 1:325667/143‑1 (MQ=255) acaTAGACGGCGGCAACTAGAGAGGCGATAATCGATGCACATACTCCAGCGGTACCGAAACCGAGATAGATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGt < 2:469678/143‑1 (MQ=255) acaTAGAAGCCGCCAAACTGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGt < 2:156574/143‑1 (MQ=255) acaTAGAAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGt > 1:384725/1‑143 (MQ=255) aaGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGGCAAAGAACTCACTGACCAGTGCCAGAAAGACGGTAATAGTgccgca > 1:235311/1‑142 (MQ=255) ccgccAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCaaa > 1:158346/1‑143 (MQ=255) cgccGAACAGTGAGGCGATAAGCGAACCACAGATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTCTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCaatg < 2:276781/143‑3 (MQ=255) gccAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAGTAGTGCCGCCAACGt > 1:354023/1‑143 (MQ=255) ccAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGtt > 1:577642/1‑143 (MQ=255) tcaGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCa < 1:695939/141‑1 (MQ=255) tGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCa < 1:839437/143‑1 (MQ=255) tGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCAACAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCa > 1:477061/1‑143 (MQ=255) cgctAATCGAACCACAAATCATCCCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAg < 2:771412/140‑1 (MQ=255) cGATAAGCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAg < 2:443790/143‑1 (MQ=255) aTAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAACGTTAAATGCCACGCAGGc > 1:1073282/1‑143 (MQ=255) aaTCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTCACGGAATGACGGTAATAGCGCCGCCAAACTTAAATGCCACACAAGcca > 2:201897/1‑141 (MQ=255) aTCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCTCCAGGTTATTGA‑TGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAAt < 1:696303/143‑1 (MQ=255) tCGAACCACAAATGCTACCGATACCGAAACCGAGATACATCACACCGTAGCCTACCGCCAGGAAATGGACCGCCAAA‑AACACACTGCCCAGAGACAGACAGCCGGAAGTAGGGCCCCCCAAGACAAAAGCCACACAGCCaagc > 2:554729/1‑141 (MQ=255) aagtGCTACCGATACCGAAACCGAGAGGACTCACACCGTAGTGTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACGGCCCAGTGACAGAAAGACGGTAATAGTGCCGCCAAATTTAAATGCCACGCAGGCAATCGCTGCAAAg < 1:362649/140‑1 (MQ=255) aTGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGaa < 1:882517/143‑1 (MQ=255) cgataccgaAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTACAAGGAACGTCACt > 1:839560/1‑143 (MQ=255) gaAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCa < 2:805266/143‑1 (MQ=255) aaaCCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCaa < 2:139667/143‑1 (MQ=255) aaaCCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCaa < 2:832483/143‑1 (MQ=255) aCCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATg < 2:205639/143‑1 (MQ=255) gaTAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCa < 1:109903/143‑1 (MQ=255) agtCACCCCGTAGTTGTTCGCCAGGTCATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAAca < 2:912539/141‑1 (MQ=255) acacCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAAcagcag < 2:357174/143‑1 (MQ=255) acacCGGAGTTTTTATCCAGGGTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAAcagcag < 2:922350/143‑1 (MQ=255) gcttttCGCCAGGTTATTGA‑GGCCAACGAGCTCACTGACCAGTGAGAGGAAGTCGGTAATAGTGCCGCCAAAGTTAAAGGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCa < 2:575548/141‑1 (MQ=255) gTTTTTCGCCATGTGATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCa < 2:616275/143‑1 (MQ=255) tttttCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCAt > 2:815410/1‑143 (MQ=255) tttttCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTACATTCACGGGTGCAACCAGCAGGGCCGCCAt > 2:658033/1‑143 (MQ=255) tttttCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGCAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCCACGAACGTCACTGCCTTCACTGGTGACAACAGCAGGGCCGCCac > 2:209002/1‑142 (MQ=255) ctccAGGCTAACGA‑GGCCGCAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGGCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACcca < 1:30635/141‑1 (MQ=255) cGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCCATGGCGCAAACAGCAGGGCCGCCATAcccc > 2:312242/1‑142 (MQ=255) cGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACcca < 1:891542/143‑1 (MQ=255) aGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCACAGAACGTCACTGCATTCCATGGTGCAACCAGCAGGGCCGCCACAcccccccg > 1:706782/1‑141 (MQ=255) ttATTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGGAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAAGCGCGGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGa > 2:995134/1‑143 (MQ=255) tATTGA‑GGCCAAAGAACTCACTGACCAGCGACAGAACGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGat < 1:129953/143‑1 (MQ=255) aTTGA‑GGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACCGCGGGGACGCCATACCCACCAGCGata > 2:414672/1‑143 (MQ=255) tGA‑GGCCCAAGAGCTCACTGAGCGGAGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATc < 2:384725/143‑1 (MQ=255) ccAAAGAACTCACTGACCAGTGACAGAAAGACGGTACTAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCCGCGATATCACCTg > 1:202395/1‑143 (MQ=255) cAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCCGCGATATCACCTGa > 1:376701/1‑143 (MQ=255) aaaGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGAc > 1:463436/1‑143 (MQ=255) aaaGAACCCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGAc < 1:201897/143‑1 (MQ=255) aaCTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGAcccct > 1:45826/1‑140 (MQ=255) gcgcACGGACCAGTCACACAGAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATg < 1:772770/140‑1 (MQ=255) tCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGCCCAATGGt > 1:436257/1‑143 (MQ=255) aCTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGtaa < 1:881764/143‑1 (MQ=255) gACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGtaataa < 2:795407/143‑1 (MQ=255) tGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGa > 1:333698/1‑143 (MQ=255) gACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACGCGGAt > 1:146473/1‑143 (MQ=255) | CACGTAGAAGGTCACATAGAAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGA‑GGCCAAAGAACTCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACGTCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGAT > NC_000913/3710892‑3711170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |