Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,060,261	G→T	7.9%	intergenic (+31/‑186)	bipA → / → yihL	ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,060,261	0	G	T	7.9%	154.5 / 4.6	64	intergenic (+31/‑186)	bipA/yihL	ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base T (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGC  >  NC_000913/4060120‑4060400                                                                                                                                              |                                                                                                                                             aTCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGc                                                                                                                                             >  2:613113/1‑143 (MQ=255)    gatgatGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGAt                                                                                                                                          >  2:565620/1‑143 (MQ=255)         tgccGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAg                                                                                                                                     <  1:531481/140‑1 (MQ=255)         tgaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAACCGATCGTCGGCGCGCCAACCGCGCACCGAAAGAGGATTAATTTCGTTTTTTTGTCATAATCGACCTGCGCGCGATgtcgg                                                                                                                                     >  1:309794/1‑139 (MQ=255)          gaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGCCGGAAAACGCACGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTGTTTAGTCATAAAAAACCTGCCAGCGATGGCAgg                                                                                                                                    >  1:765802/1‑143 (MQ=255)          gaCGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAgg                                                                                                                                    <  1:242091/143‑1 (MQ=255)           aCGAACTGGTAGAAGTGGCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCGGCGCCAACCGCGCGCCGAAAGACGATTAATTTCGTTGTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGt                                                                                                                                   >  1:74736/1‑143 (MQ=255)             gAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGAAGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAGTAATTTCGTGTGTTAGTCATCAAAAACCGCCCAGCGATGGCAggctc                                                                                                                                 >  2:816600/1‑140 (MQ=255)              aaCTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                                                                >  1:62852/1‑143 (MQ=255)                 tGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGCCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGAAGAATGGCGTGTTTTAGTCATACAAAACCTGCCAGCGATGGGAGGttgtctt                                                                                                                             >  2:756324/1‑138 (MQ=255)                  gggaGAAGGGACTCCGACCTCTATCCGTATTCGTAAACGTCAACTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                                                            <  2:332134/140‑3 (MQ=255)                  ggTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCCCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                                                            <  1:400596/143‑3 (MQ=255)                   gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATACAAAACCTGCCAGCGATGGCAGGgttttattt                                                                                                                           >  2:829910/1‑143 (MQ=255)                   gTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttt                                                                                                                           <  1:62697/143‑1 (MQ=255)                    tAGAAGTGACTCCGACCTCTAGCCGTATTCGTAAACGGCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTg                                                                                                                          <  2:854754/143‑1 (MQ=255)                     aGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttttc                                                                                                                         >  1:230236/1‑141 (MQ=255)                         gTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTTTTCTTTTTTTACCTGCCAGCGATGGCAGGTGTTTTCTTGCCTGt                                                                                                                     >  2:989595/1‑143 (MQ=255)                            aCTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGt                                                                                                                  <  2:447758/143‑1 (MQ=255)                              tCCGACCGCTATCCGTATTCGTAAGCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGa                                                                                                                <  1:366115/143‑1 (MQ=255)                                cttccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgc                                                                                                              <  1:181815/140‑1 (MQ=255)                                 ggccTCTATCCGCATTCGTAAGCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAgcg                                                                                                             <  1:593015/141‑1 (MQ=255)                                   ccgctcgccGGATGCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGca                                                                                                           <  1:855052/136‑1 (MQ=255)                                   ccTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGCTGGCAGGTTTTTTTTTGCCTCTCGTGAGCGca                                                                                                           >  1:524776/1‑143 (MQ=255)                                    ctctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcac                                                                                                          <  2:671167/143‑1 (MQ=255)                                     tctATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCGAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGcaca                                                                                                         >  1:795495/1‑143 (MQ=255)                                       tATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                                                       >  1:968928/1‑143 (MQ=255)                                       tATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGAGTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGATTATTTTTGCATGTCGTGAGCCCacaca                                                                                                       >  2:611623/1‑142 (MQ=255)                                             tATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTATTTAGTCATAAAAAACCTGCCAGCGATGGCAGGGTTCTTTTTGCCTGTCGTGCGCGGCCGCCAATGcc                                                                                                 >  2:1027364/1‑142 (MQ=255)                                              aTTCGTAATCCTCACCTGACGGAAAACGATCGTCCCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAGAAAAGACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                                                                <  2:411956/143‑1 (MQ=255)                                              aTTCGTAAACGTCACCTGACGGAAAACGCTAGTCGCCCCACCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                                                                <  1:724897/143‑1 (MQ=255)                                              aTTCGTAAACGTCACCCGACCCAAACCGCTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGa                                                                                                <  2:386231/143‑1 (MQ=255)                                                      aCGTCACCTCACGGGAAACCGTCGTCGCCGCGACAACCACGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAGCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTcc                                                                                       <  2:832523/143‑1 (MQ=255)                                                       cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAATCCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTccc                                                                                      <  2:154017/143‑1 (MQ=255)                                                        gTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCt                                                                                     <  2:62852/143‑1 (MQ=255)                                                        gTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCt                                                                                     >  2:841087/1‑143 (MQ=255)                                                         tCACCTGACGGAACACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCtt                                                                                    <  2:1076421/143‑1 (MQ=255)                                                         tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACTAATGCGAGTAT‑CTACCtt                                                                                    >  1:828305/1‑143 (MQ=255)                                                         tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGGTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCACTGCGAGTGC‑CTcccct                                                                                    >  2:137687/1‑141 (MQ=255)                                                              tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCCCACCAATGCGAGTAT‑CTTCCTTCTAAg                                                                               >  2:18193/1‑143 (MQ=255)                                                                  ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGTTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCtg                                                                           >  1:246504/1‑143 (MQ=255)                                                                     aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAACCGACCAACTGCGCTATTTAGTCATAAAAAACCTGCCAGCGATGGCAGGGTGTATGTTGCCAGACGTGAGCGCACGCAAATGCGAGGAT‑CTCCCTTATCAGTcgcggg                                                                        >  2:81459/1‑138 (MQ=255)                                                                          aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTTAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGt                                                                  <  1:839394/143‑1 (MQ=255)                                                                          aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGGTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAGGTCTGTGGGTACg                                                                   >  2:1065666/1‑143 (MQ=255)                                                                                gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTTCGTCCAtg                                                             >  1:410067/1‑142 (MQ=255)                                                                                 ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTTTCTTGAGAGCACACCAATGCGAGTAC‑GTCCGTTATCAGGAGGTGGTTACGCCACTac                                                            >  2:577360/1‑142 (MQ=255)                                                                                          ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTg                                                   >  2:776239/1‑143 (MQ=255)                                                                                           cgcgCAGCGAAAGACGATTAACTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt                                                  <  1:352602/143‑1 (MQ=255)                                                                                           cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt                                                  <  2:274006/143‑1 (MQ=255)                                                                                           cgcgCACCGAAAGACGATTAATTTCGTTTCTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACCCCAATGCGTGTAT‑CTGCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt                                                  <  1:295560/143‑1 (MQ=255)                                                                                                                       ttttAGTCATAAAGAACCTGCCAGCGATGGCAGGTTTTGTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCCATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGt                      <  1:905275/143‑1 (MQ=255)                                                                                                                         ttAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGCGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGttc                    >  1:200041/1‑142 (MQ=255)                                                                                                                           cgTCATAGAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAAt                  <  2:810783/142‑1 (MQ=255)                                                                                                                               aTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGct              >  1:156445/1‑143 (MQ=255)                                                                                                                               aTAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAA‑CTCCCTTATAGGTCTGTGGTTACGTCAATAGAGAGCTGTGTCACAATTATCTGCAAAGTCATACGCCGttgctctct              >  2:402922/1‑136 (MQ=255)                                                                                                                                 aaaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGcttt            <  1:841087/143‑1 (MQ=255)                                                                                                                                  aaaaaCCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGctttc           <  1:998424/143‑1 (MQ=255)                                                                                                                                    aaaCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCATGAGCGCACACCAATGCGAGTAT‑CTACCTTATAAGTATGTGGTTACGTCCATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGATAATTGctttctt         >  2:248787/1‑143 (MQ=255)                                                                                                                                      aCCTGCCATCGCTGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCtttt       >  1:870480/1‑143 (MQ=255)                                                                                                                                       ccTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCGATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCACAGTCTTACGACGTTAACTGCTTTCttttt      >  2:444484/1‑143 (MQ=255)                                                                                                                                        ctcccAGCGACTGCAGGTCTCTTTTTGCCTGTCGTGAGCTCACACCACTGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCtttttt     <  2:637551/140‑1 (MQ=255)                                                                                                                                        cggCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCtttttt     <  1:460815/141‑1 (MQ=255)                                                                                                                                           ccAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgc  <  2:230236/143‑1 (MQ=255)                                                                                                                                           ccAGCGATGGCAGGTTTTTCTCCGCCTGTCGTGCGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgc  <  1:539465/143‑1 (MQ=255)                                                                                                                                           ccAGCGATGGCAGGTTTGTTTTTGCCTGTCGTGAGCGCACACCAATG‑TACAATAATATGTCATTATTTTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGgc  <  1:492249/143‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             ATCGATGATGACGAACTGGTAGAAGTGACTCCGACCTCTATCCGTATTCGTAAACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT‑CTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCATACGCCGTTAATTGCTTTCTTTTTTGGC  >  NC_000913/4060120‑4060400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑