Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 475,971 C→G 9.4% intergenic (+20/+11) ybaY → / ← atl PF09619 family lipoprotein YbaY/DNA base‑flipping protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913475,9710CG 9.4% 144.2 / 9.1 54intergenic (+20/+11)ybaY/atlPF09619 family lipoprotein YbaY/DNA base‑flipping protein
Reads supporting (aligned to +/- strand):  ref base C (29/19);  new base G (2/3);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

GCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAAC  >  NC_000913/475831‑476113
                                                                                                                                            |                                                                                                                                              
gCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCACCCCCTACGCAATATTAAGCGCCGTTAACCCTCTCCGccc                                                                                                                                              >  2:1594/1‑143 (MQ=255)
   ggTACAGCCAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga                                                                                                                                           <  2:222919/143‑1 (MQ=255)
      aCAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGagag                                                                                                                                        >  1:674494/1‑143 (MQ=255)
        aGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAggg                                                                                                                                      <  1:563910/143‑1 (MQ=255)
           aaaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGGCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTta                                                                                                                                   <  1:190988/143‑1 (MQ=255)
            agccgccgTGCCGGGTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAGGGTtaa                                                                                                                                  <  2:735934/141‑2 (MQ=255)
            aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCGGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag                                                                                                                                  <  1:962963/143‑1 (MQ=255)
            aaCCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtag                                                                                                                                  >  2:800032/1‑143 (MQ=255)
             acggccgTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTCCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCGCCGCCCGGAGAGGGTtagt                                                                                                                                 <  1:709018/140‑1 (MQ=255)
                  cgTGCCGGTTAAGGTCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGATGAATCCGTCTTAGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTc                                                                                                                            <  2:273347/143‑1 (MQ=255)
                     gCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAATCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAg                                                                                                                         <  1:516526/143‑1 (MQ=255)
                         gTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTGCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCCATATTAAGCGACGTTAACCCTCTCCGCCCCGAGAGGGCTCGTAGTTCCAGCCAt                                                                                                                     >  2:227344/1‑143 (MQ=255)
                               gCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCCGGTGAATCCGGCATCGACAGATCCAGCTCCTGCGGACTATTAAGCGACGTTAACCCTCTCCGACCGCGGAGGGCTAGACGCTCCAGCGATGACGcg                                                                                                               >  2:190304/1‑142 (MQ=255)
                                 cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCCGCTCCTACGCAATATTAAGCGACGTTAACCCGCTCCGCCCGGAGAGGGTTAGGAGTTCCAGCGATAACGCTGc                                                                                                             >  2:213255/1‑143 (MQ=255)
                                 cAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTGCGGCAGTTCCAGCTCCGACGCAATATTAAGCGACGTGAACCCGCCCCGCCCGGGCCGGGCTAGGAGTTCAAGCGAGAACGCTGc                                                                                                             >  2:960836/1‑143 (MQ=255)
                                    cGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCAAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAg                                                                                                          >  2:545105/1‑143 (MQ=255)
                                     ggTGGCCCAACGCCTCCCGTACCTTCGACTTCACCATCTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGGTAACGCTGCAAGt                                                                                                         <  2:772832/143‑1 (MQ=255)
                                       tGGCGCAGCGGCTGCCGTACCTTCGACTTGCGCAACTCAGGTGAATCCGTCTTCGGCCGTTTCAGCTCCTACGCGGGATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg                                                                                                       <  2:374208/143‑1 (MQ=255)
                                           gcAACGCCTACCGTACATTCGGCTTGACCAGCTCGGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGAttt                                                                                                   <  2:257450/143‑1 (MQ=255)
                                             aaCCACTACCGTCCCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGc                                                                                                 <  1:981717/143‑1 (MQ=255)
                                              aCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGGTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGcc                                                                                                <  1:432471/143‑1 (MQ=255)
                                                gACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTACAGCTCCTACGCACAATTCAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTGAGTAGATCCACACATAATGTATCAAGTCGATTCGcacc                                                                                              >  2:417751/1‑140 (MQ=255)
                                                 ccTACCGTCCTTTCGACTTCACCACCTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGc                                                                                             <  2:631156/142‑1 (MQ=255)
                                                       gTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAgg                                                                                                                                       <  1:112222/95‑1 (MQ=255)
                                                       gTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGGGCGGAGAgg                                                                                                                                       >  2:112222/1‑95 (MQ=255)
                                                            ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGGTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATAcc                                                                                  >  1:24180/1‑143 (MQ=255)
                                                            ttCGACTTCACCAACTCAGGTGAATCCGTCTTCGACAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTAAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCACGATaac                                                                                  >  2:432042/1‑141 (MQ=255)
                                                             tCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCCATATTAAGCGATGTTAACCCCCTCCGCCCGGCGAGGGCTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTATCCCGCCTCCCCACACCa                                                                                 >  2:1027849/1‑143 (MQ=255)
                                                               gACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCCATATTAAGCGACGTTAACCCTCTCCGCCCGGCGAGGCTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCCTc                                                                               >  1:806357/1‑143 (MQ=255)
                                                                aCTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCACCGCCCGGAGAGGGTAAGTAGTTCAAGCGATAAAGGCGCAACTCGATTTGACAGCTCCCCGACAccacca                                                                              >  2:623305/1‑143 (MQ=255)
                                                                  ttCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATATGCCCGCAGCCCGATACCATcacc                                                                            >  2:659631/1‑142 (MQ=255)
                                                                          cTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGc                                                                    <  2:695788/143‑1 (MQ=255)
                                                                           tCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACCCCTTccccc                                                                   >  1:133957/1‑139 (MQ=255)
                                                                               gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAgca                                                               >  2:988340/1‑141 (MQ=255)
                                                                               gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGCAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTa                                                               >  2:988345/1‑143 (MQ=255)
                                                                                tGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTaa                                                              <  1:747302/143‑1 (MQ=255)
                                                                                        tccccGGCAACTCCACCTCCAACGCCATCACACGCGACGTCAACCCACGCCCCCCGGAGATGGTGAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCAACACACCTTCTGCCAGTAATGCCTGTc                                                      <  1:344956/139‑1 (MQ=255)
                                                                                        tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCACGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGtt                                                      >  1:595043/1‑142 (MQ=255)
                                                                                          ttCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCACGTCGCTTTGCCCGCTTCCCGATACCCTCCCCCCTCCTGACAGCAATGCCTGCCGc                                                    >  2:124842/1‑143 (MQ=255)
                                                                                             ggCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGCAAGTAGTTCCAGGGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTCAAAGTAATGCCTGTcacaga                                                 >  2:690557/1‑138 (MQ=255)
                                                                                              gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                >  1:694309/1‑143 (MQ=255)
                                                                                              gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                >  2:446972/1‑143 (MQ=255)
                                                                                              gCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCCGCAAGTCGATTTGCCCGCTTCCCGATACCATCCCACCTTCTGCCAGTAATGCCTGTCGCTGAc                                                >  1:14293/1‑143 (MQ=255)
                                                                                               cAGTGCCGGCTCCTCCGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACg                                               <  2:598862/143‑1 (MQ=255)
                                                                                                aGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCAAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTACTGCCAGCAATGCCTCTCGCTGACGc                                              >  2:484991/1‑143 (MQ=255)
                                                                                                 gTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCt                                             <  1:800032/143‑1 (MQ=255)
                                                                                                                cAATATTAAGCGACGCTAACCCTCGCCGCCCGGAGAGGGTTAGTAGTTCCAGCGACAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAAtccggtccgg                              <  2:99882/143‑1 (MQ=255)
                                                                                                                 aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCAAGTAATGCCTGTCGCTGACACCGTAAAtccggtccgtt                             >  2:17539/1‑141 (MQ=255)
                                                                                                                          cGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAt                    <  1:614673/143‑1 (MQ=255)
                                                                                                                           gACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCCGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAAtt                   >  1:187391/1‑143 (MQ=255)
                                                                                                                                  aCCCTCTCCGGGCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGt            >  1:96108/1‑143 (MQ=255)
                                                                                                                                   cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCCGTTAGCGCAATTGTGCCGTg           >  2:659275/1‑143 (MQ=255)
                                                                                                                                   cccTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGGAAATCCGGTCCGGTTAGCGACATTGTGCCGTg           >  2:964990/1‑143 (MQ=255)
                                                                                                                                   cccTCTCCGCCCGGAGAGGGGTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTCCTGTCAGTCATGCCTGTCGCTGACGCTGTATATCCGTTCCGGGTCGCGCAATTCTTCCGTg           >  1:500521/1‑143 (MQ=255)
                                                                                                                                     ctctCCGCCCGGAGAGGGTGAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATGCGGTCCGGTTGGCGAAATTGTGCCGTggg         >  1:421709/1‑142 (MQ=255)
                                                                                                                                            cccGGAGAGGGTTAGGAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAac  <  1:846631/143‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
GCCGGTACAGCAAACCGCCGTGCCGGTTCAGGCCAGCGGTGGCGCAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCGATTTGCCCGCTTCCCGATACCATCACACCTTCTGCCAGTAATGCCTGTCGCTGACGCTGTAAATCCGGTCCGGTTAGCGAAATTGTGCCGTGGCGATTAAC  >  NC_000913/475831‑476113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑