Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp		insH21	insH21

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	55 [0]	[0] 55	insH21	insH21

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	50 (1.590)	47/246	0.0	100%	intergenic (+253/‑33)	ychE/insH21	putative inner membrane protein/IS5 transposase and trans‑activator
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+151/‑484)	insH21/oppA	IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein

AGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1299367‑1299498 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTA  >  NC_000913/1300698‑1300824                                                                                                                                                                                                                                                                     AGTTATCAGGATTTCCTTAACTGCTTCTCCTCGCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGC                                                                                                                                    <  2:152208/129‑1 AGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGC                                                                                                                                    <  2:614580/129‑1 AGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGGGC                                                                                                                                    >  2:581567/1‑129   TTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAAGTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                                                                                                                  <  2:590090/129‑1    TATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGAAAAATTAACAACATAATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                                                                                                 >  1:380596/1‑129      TAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCGACATCATAATCCTGGGCTTGCGGAAGAATAATTGAAATGATATTATTAATTCCACTGACTTTGGTAGAGGAAAGGACTAAAT                                                                                                                               >  2:175899/1‑129        agtATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGTGCTTGCTGAAGATTAATTTAAATTTTATTATTAATTCCAATGCCTGTGGTAGAGGAAAGTGCTAAATAA                                                                                                                             <  1:39271/126‑1        AGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAA                                                                                                                             >  1:192071/1‑129                CTTAACTGCTTCGCCTCACCATCATGTTATTTTCGCCACATCATAAACCTGGGCTGGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTCAATAATAATCAAT                                                                                                                     >  2:34434/1‑129                    ACTGCTTCTCCTCACCCTCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATCATAATCAATTGTT                                                                                                                 >  1:422517/1‑129                      TGCTTCTCCTCACCATCCTGTTATTTTCGCCACATAATAAACATAAAATTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAA                                                                                                               >  1:271937/1‑129                       GCTTCTCCTCACCATCTTGTTATTTTTGCCACAGCATAATCCTGGAATAGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGGAAGTGCTAAATAATAATCAATTGTTAAA                                                                                                              >  1:495891/1‑129                              CTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGCATAATTGAAATGATATTATTACTTCCACTGCCTTTGATAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATCGT                                                                                                       >  2:534183/1‑129                               TCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTATG                                                                                                      >  2:304222/1‑129                                      CATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAAATGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCA                                                                                               >  1:554420/1‑129                                        TGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTACCTTTGGTAGAGGAAAGTGTTAATTATTAATCAATTGTTAAAGTATTGTGCATTTCACT                                                                                             <  1:383237/129‑1                                          TTATTTTCGATACATCATAATCCTGGGTTTGATTATGATTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTAC                                                                                           <  2:313109/129‑1                                           TATTTTCGCCACATCATAATCCTGGGCTTGCAAAAAAATAATAGAAATAATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACT                                                                                          >  1:514181/1‑129                                           TATGTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACT                                                                                          <  2:386623/129‑1                                             TTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGG                                                                                        >  1:296943/1‑129                                               TTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAA                                                                                      >  2:540452/1‑129                                                 CGCCACATCAGAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTTCACTGCCTTTGGTAGAGGAAAGTGCTTATTAATAATTAATTGTTAAATTATTGTGCAGTTCACTACTGGAACT                                                                                    <  1:354894/129‑1                                                    CACATCATAACCCTGAGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGTTAAATATTAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTA                                                                                 <  1:260124/129‑1                                                     ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATTATATTTATTTGTTATATTATTGAACAATTCACTACTGGAACTGTAA                                                                                <  1:621834/129‑1                                                      CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTACACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAT                                                                               >  1:660086/1‑129                                                          ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGATAGTGCTATATAATAATTAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGA                                                                           <  1:111060/129‑1                                                             ATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAA                                                                        <  2:46667/129‑1                                                              TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAG                                                                       >  2:595229/1‑129                                                                 TGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTACACTGCATTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATA                                                                    >  1:351123/1‑129                                                                  tGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTTTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAG                                                                   <  1:400826/128‑1                                                                           GAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTA                                                          <  1:540452/129‑1                                                                                       AAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAT                                              <  2:402357/129‑1                                                                                        AATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATT                                             >  1:455176/1‑129                                                                                        AATGATATTATTAATTCCACTGCCTTTGGTAGAAGAAAAAACAAAATAATAATCAATTGTTAAATTATTGTGCATTGCACTACTGGAACTGTAATAAGAAAAGAAAGACATGCTTAGCCAATCTCTATT                                             >  1:138752/1‑129                                                                                               TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGA                                      <  2:547185/129‑1                                                                                               TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTAAATAAAAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGA                                      >  1:154556/1‑129                                                                                                  TTAATTCCACTGCCTTTGGTATAGGAAAATACTAAATAATAAACAATAGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATT                                   >  1:319571/1‑129                                                                                                  TTAATTCCACTGCCTTTGGTAGAAGAAAATACTAAATAATAATAAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATT                                   >  1:299542/1‑129                                                                                                   TAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTATTAAAATATAATGCATAACACTAATGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATGTGATTGAATag                                  >  1:86817/1‑127                                                                                                    AATTCCACTGCCTTTGGTAGAGGAAAGTTCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGA                                 <  2:499219/129‑1                                                                                                     ATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAACAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAA                                >  2:348188/1‑129                                                                                                      TTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATAATTAAATTATTGTACATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTCTATTTGATTGAATTGAAA                               >  1:75944/1‑129                                                                                                       TCCACTGCCTTTGGTATAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAG                              <  2:263758/129‑1                                                                                                         CACTGCCTTTGGTAGTGGTAAGTGCTAAATAATAATAAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAT                            >  1:198912/1‑129                                                                                                          ACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTAAATTGAAAGATG                           >  2:294967/1‑129                                                                                                             GCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAAAAGAAAGAGATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTT                        >  1:456402/1‑129                                                                                                              ccaTTGGTAGAGGAAAGTGATAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTG                       <  2:375110/126‑1                                                                                                               CTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGATCTGTAATCAGTAAAGATATACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGT                      <  1:54722/129‑1                                                                                                                TTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCACTCTCTATTTGATTGAATTGAAAGATGTTTGTc                     >  2:22060/1‑128                                                                                                                     TAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGC                <  2:271937/129‑1                                                                                                                          GAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCACTATTTGATTGAATTGAAAGATGTATATTAAGGCATGGA           >  2:32109/1‑129                                                                                                                            AAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGTAAAGATAGACATGCTTAGCCAATCTTTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATG         <  1:501450/129‑1                                                                                                                              GTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCA       >  1:486727/1‑129                                                                                                                               TGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAAAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAA      >  1:340361/1‑129                                                                                                                               TGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAATAATGGAATTGAAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAA      >  1:72908/1‑129                                                                                                                                gcgAAATAATAATAAATTGTTAAATTATTGTACACTTCATTACTGGAATTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAACGCATGGATGCAAG     <  2:260733/126‑1                                                                                                                                  TAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCT   >  2:265397/1‑129                                                                                                                                  TAAATAATAATCAATTGTTAAATTATTGTGAATTAAACAACTAGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCT   >  1:374954/1‑129                                                                                                                                   AAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGACAGCAAGCga  >  2:309263/1‑127                                                                                                                                                                                                                                                                     AGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1299367‑1299498 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTA  >  NC_000913/1300698‑1300824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.