| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 95.0% | 37.7 / NA | 21 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (8/11); minor base G (0/1); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCA > NC_000913/124121‑124362 | aaaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGTAAAGCGACAGTAATCCTTGCTCATACTATTATAGGTTACGGTATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAg < 1:267423/129‑1 (MQ=255) cTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAAt > 1:315403/1‑129 (MQ=255) tGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGTTTAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAt < 1:562722/129‑1 (MQ=255) aaaGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGtgtg > 2:375762/1‑129 (MQ=255) cAGGAAACCAAAGGCAAAGCGACAGTAATCCTGGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCat < 2:501298/129‑1 (MQ=255) ggAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCTCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCatat < 1:488604/129‑1 (MQ=255) aaCCAAAGGCAACGCGACAGTAATCCTTGCTCATACCATTAAAGATTACAGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGTTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg > 1:376080/1‑129 (MQ=255) ataGGCAAAGCGACAGTAATCCTTGCTTATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGAc < 2:71105/127‑1 (MQ=255) gCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCACGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGtt > 2:241040/1‑129 (MQ=255) aGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCAATAGGTTAAGAAAAAGAACATGGACGGTGTGCGTCATATCAGCGAACGTGTCaa > 2:459474/1‑129 (MQ=255) gCGACAGGAACCCTGGCTCATACCAGTAGAGGTTACGGGATGGGCGACGCGGCTGAAGGTAAAAACATCTCGCTCTAGGTTAATTTAATGAACATGGACGGTGTGCGGCATATCCGGGACCGTTTCAAt < 1:89282/129‑1 (MQ=255) cAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCTACGCGGCTGAAGGTAATAATATCGTTTATTTTGTTATTTAAATTATTATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc < 1:66884/129‑1 (MQ=255) aTCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGTTGAAGGTAAAAACGTCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAACGTGCCGgtg > 2:391439/1‑129 (MQ=255) tCCGTGCTCGTGCCATTAAAGGTTACGGCATGGGCGGCGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGTAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGgtgt < 2:424115/129‑1 (MQ=255) cATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATAACACAATAGGTAACGAAAAAGAACATAGAAGAAGAATCACATATCCGCGACCGTTTCAATGTGCCGGTGTCTGGTGCAGatat > 1:618210/1‑129 (MQ=255) tttAGAGGTGACTGCCTGGGCGACGCTGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATAGCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATc < 2:207073/128‑1 (MQ=255) cGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGcc > 1:396850/1‑129 (MQ=255) ggCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACAGCGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCg < 2:358957/129‑1 (MQ=255) gCTGAAGGTAAAAACATCGCGCACGAGGTTAAGAAACTGAAGATGGACGGTGTGCGTCATATCCGCGCCCGTTTCAAGGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCg < 2:618210/129‑1 (MQ=255) tAAAAACATCGCGTACTAGGTTGAGACAATGATCAGGGCCGGTGTGCGTCAGCTCCGCGACCGTGTGAATGTGCCGGTGGCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTc < 2:58647/129‑1 (MQ=255) cgcgCAACAGGTTGAGAAAATGAAGATGGATCGTGTGCGTCAGATCCGAGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAATCTGCCGTACATCACCTTCCCGGAGGGTTCTGAAGAGCa < 2:382071/129‑1 (MQ=255) | AAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCA > NC_000913/124121‑124362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |