| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,934,874 | T→A | T481S (ACC→TCC) | zwf ← | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,934,874 | 0 | T | A | 96.7% | 69.7 / NA | 30 | T481S (ACC→TCC) | zwf | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (10/19); total (10/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTT > NC_000913/1934750‑1934987 | cGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTACACAAAAAAAAAAAACAGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTACAGGAACGACCATCACTGGAAatc > 1:330432/1‑129 (MQ=255) cACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGAACATGACAGGAAatgac > 2:180041/1‑126 (MQ=255) gAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGc < 2:77757/129‑1 (MQ=255) cTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGTTTAAGCTTAGTTATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCAGCGCCACCg < 1:354888/129‑1 (MQ=255) gAAGGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCCGGAACGACCATCACGGGAAATCATCGCCACCGAg < 2:41314/129‑1 (MQ=255) gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGTAATGACCATCACGGGAAATCATCGCCACCGAGGCAACg < 1:280020/129‑1 (MQ=255) tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCTAGGATTGACTATCACGGGAAATCATCGCCACCGAGGCAACgg < 1:478040/129‑1 (MQ=255) aataaTTGTTGTGCTATCTGCGAGATATCATGTTACCGGTAAAATAGCCATACCGGAGATGCGCAGATATTACTCAACCTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACggg < 2:108843/126‑1 (MQ=255) aaaTAGTTCTACAAGCGGCGCAAGATCATGTTACCGGAAAAATAACCATCAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACAAACACGGCAAATAATCGCCACCCAAGCAACcggcc > 2:242081/1‑127 (MQ=255) aaTCGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTcc < 2:463983/129‑1 (MQ=255) tttGTTCTACAATCTGCGCAAGATCTTGTTACCGGTAAAATAACCATAAAGGATAATCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTccc < 2:177765/128‑1 (MQ=255) aTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGTGAAAGCATCCCCACCGAGGTAACGGGTacc > 2:148377/1‑129 (MQ=255) gTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCACTCCAGGAACGACCATAACGGCAAACCATCGCCACCGCGGCACCGCGTCCACAg > 2:596542/1‑129 (MQ=255) acatgcTGCGCAAGATATTGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAGTCATCGCCACCGAGGCAACGGGTCCCCAGGTTcc < 2:235613/124‑1 (MQ=255) cTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGATCATTTCTGTTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt < 1:200866/129‑1 (MQ=255) gATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGACCCCAGGCACCGGCCTCCTACggc > 2:110128/1‑128 (MQ=255) gATCATGTTACCGGTAAAATAACCATAAAGGATAAGCACAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATAATCGACCCCTATGTAACGGGTCCCCAGGTTCCGGCCTGATACGGt > 1:417980/1‑129 (MQ=255) tGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg < 2:410068/129‑1 (MQ=255) aaGGATAAGGGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc < 2:1090/129‑1 (MQ=255) aaGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGTGTCCCCAGGTTCCGGCTTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc < 1:9712/129‑1 (MQ=255) tatTACTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAg < 2:384461/129‑1 (MQ=255) ttACTCAAACTCCTTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCTTCCTTATACTGTTTTGGTTTATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTc < 1:48337/129‑1 (MQ=255) cTCAAACTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACTGTTTCGGCGCATCATTGTCCATCGCCCACGCCACAGTAATGGAGTCTAc < 1:464058/129‑1 (MQ=255) aagcgcATTCCAGGAACGACCAGCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCa < 2:45410/124‑1 (MQ=255) cTCATTCCAGGAACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATTGCCCACGCCTCAGTAATGGAGTCTACCCAttt < 1:452130/129‑1 (MQ=255) ttCCAGGAACGACCATCACGGGAAATCATCGCCACCAAAACAAAAAATCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAg > 1:631108/1‑129 (MQ=255) tcccAGGCACGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAAGTTCCGGCCTAATACGGTTTCTGCGCATCATTGTTTATCGTCCACGCCTCAGTAATGGAGTCGACCCATTTCCAg < 1:319035/127‑1 (MQ=255) ggAACGACCATCCCGGGACATCTTCGCCACCGAGGCAACGAGTCCCCAAAATCCAGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCAACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttc > 1:100893/1‑129 (MQ=255) aaCGACCATCACGGGAAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGTCCATGCCTCAGTAATGGAGTCTACCCATGTCCAGGcttctt < 1:110128/129‑1 (MQ=255) aaCGACCATCACGGGAAATCATCGCCACCGAGGCAACGGATCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttctt > 1:547944/1‑129 (MQ=255) | CGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTT > NC_000913/1934750‑1934987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |