Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,711,031	G→A	P326L (CCG→CTG)	yhjX ←	putative pyruvate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,711,031	0	G	A	100.0%	79.5 / NA	32	P326L (CCG→CTG)	yhjX	putative pyruvate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/19);  total (13/19)

AAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCT  >  NC_000913/3710911‑3711148                                                                                                                         |                                                                                                                       aaGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTTGATCGTTTACAACGACAAAAAAACACACTGACCCGTGACAGAAAGACg                                                                                                                >  2:553393/1‑129 (MQ=255)    ccgccAAACAGTGAGGCGATGCTCGAACGACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTa                                                                                                             <  1:376618/129‑1 (MQ=255)      gccAGACAGTGATGGGATAATCGAGCCACAGATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAAt                                                                                                           <  2:207579/129‑1 (MQ=255)       ccAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATa                                                                                                          >  1:396389/1‑129 (MQ=255)       ccAAACAGTGAGGCGATAATCGAACCAAAAATGCTACCGATACCGAAACCGAGATAAATCACATCGTAGTTTTTTGTTTTGTTATTGAGGTCAAAGAACTCACTGAACAGTGACAGAAAGACGGTAATa                                                                                                          <  1:177681/129‑1 (MQ=255)               tGAGGCGATAATCGCACCACAAATGCTACCGATAAAAAAAACAAAAAAAATCAAACCGTAGATTTTCGCCAGGTTATTGAGGACAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTgccgcc                                                                                                  >  1:276857/1‑129 (MQ=255)                   ccggtAATCGAACCACGAAGGCTTCCGAGACCGAAGCCGAGATAAAGCACGCCGTAGTCTTTCGCCAGGTTATTGAGGTCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCTAAg                                                                                              <  2:103771/125‑1 (MQ=255)                          tgtaaCCACAATTGCTACCGATACCGAAACCGAGATAAATGACACCGCAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATg                                                                                       <  2:392852/126‑1 (MQ=255)                                 acaAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCTAATGAATTCACTGACTAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCa                                                                                <  1:119342/129‑1 (MQ=255)                                  caAATGCTACCGATTCCGAAACCGAGATAAATCACACCGTAGTTTTGCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAg                                                                               <  2:94450/129‑1 (MQ=255)                                    aaTGCTACCGCTACCGAAACCGAGATAAATCACACCATAAACTTTCGCCAAGATAATAAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGc                                                                             >  1:549494/1‑129 (MQ=255)                                                accgaAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGATATTGAGGACAAAGAACTCACTGAACAGTGACAAAAAGACAGTAATCGTGACGCCACAGTAAGATGCCACGCACGCAATGGCCGCagc                                                                 >  2:574201/1‑127 (MQ=255)                                                 ccgaAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAATGTTTTTTTTTGTGTCGGCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAg                                                                <  1:620720/129‑1 (MQ=255)                                                        cGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCa                                                        >  1:498320/1‑129 (MQ=255)                                                         gagaTAAGTCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTTACAGAATTTCGTTAATATTGTTTTTATTGTTTAATGTCACGCAGGCAATCGCTGCAAAGAACG‑TCAc                                                       <  1:569177/129‑1 (MQ=255)                                                           gaTAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCCGTGACAGAAAAACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCCGGAAAACGCTGAAAAGAAAG‑GCAcag                                                     >  2:206409/1‑127 (MQ=255)                                                             tAAACCACACCGTAGTGTTTCGCTGGGTTATTGCGGCCGACGATCTCACTGACCAGTGACAGAATGACGGTAATCGTGCCGCCCAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCa                                                   <  2:612485/129‑1 (MQ=255)                                                                aTCACACCGTAGTCTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGTTAATAGTGCCGCCATATTTAATTTCTATGCAGGAAATCGATGCAAAGAACG‑ACACTGCATTc                                                <  1:543380/129‑1 (MQ=255)                                                                         tAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCa                                       <  1:553393/129‑1 (MQ=255)                                                                               ttCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATAACTGCAAAGAACG‑TCACACCATCCAATGGAACAAAcagc                                 >  2:154490/1‑129 (MQ=255)                                                                                     aGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGAAAAAAGGAAATGAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGgcc                           >  2:112576/1‑129 (MQ=255)                                                                                        ttATTGAGGCCAAAGAACTCTCTGACCGGTGACAGAACGACGTTAATATTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGgccgcc                        <  1:574201/129‑1 (MQ=255)                                                                                         tATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACTGTAATAGTGCCGCCAAAGTTAAATTCCACGCAGGCAATCGTTGTAAAGAATG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCa                       <  1:184326/129‑1 (MQ=255)                                                                                         tATTGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCTGGCAATCTTTGCAATGTACG‑TCATTGCATTCAATGGTGCAAACAGCAGGGCCGCCa                       <  1:194354/129‑1 (MQ=255)                                                                                           ttGAGGCCAAAGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATa                     >  2:338866/1‑129 (MQ=255)                                                                                              aGGCCAAAGAACTCACGGACCAGTTACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATAccc                  <  2:209300/129‑1 (MQ=255)                                                                                                gCCAAAGAACACACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACccac                >  2:314954/1‑129 (MQ=255)                                                                                                  cAAAGAACTCACTGCCAACTAACAGAAAGCCGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGTACG‑TCTCTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACccacca              <  1:374274/129‑1 (MQ=255)                                                                                                   aaaGAACTCACTGACCAGTGACAGTAAGACGGTAATAGTGCAGAAAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAg             >  1:336222/1‑129 (MQ=255)                                                                                                    aaGAACTCACTGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAATTACG‑TCTTTGTATTTATTTGTTCAAACAGCAGGGCCGCCATACCCACCAGc            <  1:15726/129‑1 (MQ=255)                                                                                                            aCTTACCGGGGACAGAAAGACTGTAATAGTGCCTCCAAATTTAAATGCCACGCACGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCAc    <  2:150680/129‑1 (MQ=255)                                                                                                              tGACCAGTGACAGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCAAGCAGGCAATCGCTG‑ATCGCGCGCAGACGGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCt  >  1:458727/1‑129 (MQ=255)                                                                                                                         |                                                                                                                       AAGCCGCCAAACAGTGAGGCGATAATCGAACCACAAATGCTACCGATACCGAAACCGAGATAAATCACACCGTAGTTTTTCGCCAGGTTATTGAGGCCAAAGAACTCACTGACCAGTGACGGAAAGACGGTAATAGTGCCGCCAAAGTTAAATGCCACGCAGGCAATCGCTGCAAAGAACG‑TCACTGCATTCAATGGTGCAAACAGCAGGGCCGCCATACCCACCAGCGATATCACCT  >  NC_000913/3710911‑3711148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑