| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 95.5% | 44.0 / ‑5.4 | 22 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (11/10); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
AGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAC > NC_000913/760369‑760601 | aGGAGCTGGCGAAACGCTTCAGCACGGTAACAGAAAAAGAAGAAATGAAGTCTCGAGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa > 1:368210/1‑129 (MQ=255) ctgcaggCGACACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGACTAGCGATACCAAGAATTATGGCAATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAacac > 1:212868/6‑129 (MQ=255) gAAACGCATCAGCACGGTGCCGGAACCAGTTAAAATGCAATCAAGCGATGCAAAGAAAAATAGCGATCGCCAGGCGATGACTGCCGGTGAGAAACTGTTCGACTGGAGCAGTGCGGAAAACATCGCTTa > 1:566548/1‑129 (MQ=255) ccGGACGCAGTTGACATGCAGTCTCGCTTTACCAAAAAATATGACAAAAAACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa > 1:69179/1‑129 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg < 1:52143/129‑1 (MQ=255) aGTTGAAACGCAGCCTCGCGTTGCTAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTGGAGCCGTGCGGCAAACCTCGCTTACGCCACGCTGGTTGATTAACGCATTcc < 2:45339/129‑1 (MQ=255) aGTCGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCTACTTTATTTGTTTGTAAATTCTCGCTTACGCCACGCTGGTGGATGAAGGCATTcc < 1:493608/129‑1 (MQ=255) gtacaTGCAGTCTCGCGTTGTCAAGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt < 2:555/125‑1 (MQ=255) aaaTGCTGTCTCGCGTTGCCGAGTTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGATACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTc < 2:337456/129‑1 (MQ=255) gTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCAGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTc > 1:661207/1‑129 (MQ=255) tctcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGACGGTGAGAAACTGTTCGACTGGAGCGGAGCGGAAAACCTCGCTTACGCAAAGCGGGTGGGTGAAGGCATTCCGGTACGccgggcg > 2:160645/1‑124 (MQ=255) cgcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTAAGAAACTGATCGACAAGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGt > 1:330984/1‑129 (MQ=255) aaGATTTATGGCCATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCTACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc < 2:512735/129‑1 (MQ=255) aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCTACGCTGGGTGATGAAGGCATTGCGGTTCGCCCGTCGGGTGAAGACTCCGGTCGCGGTaacc > 2:57351/1‑126 (MQ=255) aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCAACAAAAAAGAAACGGAAAAACTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTGGCGGTACct > 1:271284/1‑129 (MQ=255) tCGCCAGGCGATGGCTGCCGGTGAGAAACTATTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACctt > 2:294377/1‑129 (MQ=255) aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttcg > 1:268367/1‑128 (MQ=255) gCGATGGCTGCCGGTGAGAAACTCTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTTCGGTTTGTTTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:331403/129‑1 (MQ=255) cTGCCGGTGAGAAACTATTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGcgtgg < 1:294377/129‑5 (MQ=255) gaAACTGTTCGACTGTAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCAACGCGGTGATCca < 2:661207/129‑1 (MQ=255) cTGTTCGACTGGGGCGGTGCGGAAACCCTCGCTTACGCCTCGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCGTCCACCGCCACGCGGTGATCCACAAc < 2:222369/129‑1 (MQ=255) cTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGTTTTATGATTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc < 1:194817/129‑1 (MQ=255) | AGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAC > NC_000913/760369‑760601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |