Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	95.0%	34.1 / ‑6.9	20	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (12/7);  minor base G (1/0);  total (13/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

TCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACC  >  NC_000913/1196105‑1196292                                                                                                                    |                                                                         tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGCCAAAGTAAATCCTGGCTCTATCATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGActg                                                             >  2:446130/1‑127 (MQ=255)  cGGTGACGAATGCGCTCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGActga                                                            <  2:31992/129‑4 (MQ=255)  cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGa                                                            >  2:148053/1‑129 (MQ=255)        cGAATGCGCCCTGTTTGAAGCCACCCACGATACTGCGCCGAAATATGCCGGTCAGGAAAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCACCATATGGGTTGGACTGaaacagc                                                      >  2:148910/1‑124 (MQ=255)         gAATGCGCCCTGTTTGCAGCCACCCACGGTACTACGCCGAAATATACCGATCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCt                                                     >  1:223218/1‑129 (MQ=38)         gAATGCGCCCTGTTTGAAGCCAGCCACGGTACTGTGCCGGAATATGTCGGTCAGGACAAAGTAAATCCTGGCTCTTTTATTCTTTCCGCTGAGTTCATGCTGCGCAATATGGGTTGGACTGAAGCGGCt                                                     <  1:624947/129‑1 (MQ=255)           aTGCGCCCTGTTTGCAGCCCCCCACGGTACTGCGCCGAAATAAACAAAACAAAAAAAAGTAAATCCTGGCTCTATTATTCTCACCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGa                                                   >  1:100846/1‑129 (MQ=25)                        gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                      <  2:226017/129‑1 (MQ=37)                             cacccacGGTACTGCGCCGCACTATGCCGGTCAGGACAAAATAAATCATAGCTCTATTATTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                 >  1:427222/1‑129 (MQ=21)                             cacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTCTTAAAGGTAt                                 >  2:594435/1‑129 (MQ=37)                                 cacGGTACTGCGCCGCAATATGCCGGTCAGGACAAAAAAAATAAAAGCACTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                             >  1:474769/1‑129 (MQ=18)                                   cGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGATATGATTCTGTTCCATATGTGTTTGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                           <  1:251735/129‑1 (MQ=25)                                       aCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGTGGCTGACCGGATTGTCAAAGCAGTGGAAGccgga                       >  2:568394/1‑127 (MQ=25)                                                  atatGCCTGTCAGGACAAAGTAAATCCTTGCTCTATTATTCTCTCCGCTGAGATGATGCTGTGCTATTTGTGTTTGTCTTAATCGGCTGATCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa            <  1:184547/129‑1 (MQ=16)                                                       ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg       >  2:107393/1‑129 (MQ=17)                                                       ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg       >  2:460575/1‑129 (MQ=17)                                                          gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGTTGTCTTTTTTTTTATTTTTTTGGAAGGCGCAATCAAGGCCAAGACCGTaa    <  1:279013/129‑1 (MQ=18)                                                          gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATACTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGCATCGAAGGCGCAGTCAATGCCAAGAcccgaa    >  2:26482/1‑125 (MQ=17)                                                           tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGTCCTTTTTTTTTTTGGTATGGAAGGCGCAAGCAATGCCAAGACCGGAAc   <  1:299885/129‑1 (MQ=12)                                                            cAGGAAAAAGTAAATCCTGGCTCTAAAATTCACGCCGCGGAGATGATGCTGCACCAGATGGGTTGGACTGAAGCGCCTGACCTGATTGTTAAAGGTAAGGAAGGGGCAATCACAGCAAAACACGTAAcg  >  2:261040/1‑128 (MQ=9)                                                                                                                    |                                                                         TCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACC  >  NC_000913/1196105‑1196292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑