Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,245	T→C	L375M (TTA→CTG)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,245	0	T	C	92.3%	19.7 / ‑6.4	13	L375M (TTA→CTG)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (8/4);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACC  >  NC_000913/1196128‑1196292                                                                                                                      |                                                gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                      <  2:226017/129‑1 (MQ=37)      cacccacGGTACTGCGCCGCACTATGCCGGTCAGGACAAAATAAATCATAGCTCTATTATTCTCTCAGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                 >  1:427222/1‑129 (MQ=21)      cacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTCTTAAAGGTAt                                 >  2:594435/1‑129 (MQ=37)          cacGGTACTGCGCCGCAATATGCCGGTCAGGACAAAAAAAATAAAAGCACTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa                             >  1:474769/1‑129 (MQ=18)            cGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGATATGATTCTGTTCCATATGTGTTTGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                           <  1:251735/129‑1 (MQ=25)                aCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGTGGCTGACCGGATTGTCAAAGCAGTGGAAGccgga                       >  2:568394/1‑127 (MQ=25)                           atatGCCTGTCAGGACAAAGTAAATCCTTGCTCTATTATTCTCTCCGCTGAGATGATGCTGTGCTATTTGTGTTTGTCTTAATCGGCTGATCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa            <  1:184547/129‑1 (MQ=16)                                ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg       >  2:107393/1‑129 (MQ=17)                                ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg       >  2:460575/1‑129 (MQ=17)                                   gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGTTGTCTTTTTTTTTATTTTTTTGGAAGGCGCAATCAAGGCCAAGACCGTaa    <  1:279013/129‑1 (MQ=18)                                   gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATACTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGCATCGAAGGCGCAGTCAATGCCAAGAcccgaa    >  2:26482/1‑125 (MQ=17)                                    tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGTCCTTTTTTTTTTTGGTATGGAAGGCGCAAGCAATGCCAAGACCGGAAc   <  1:299885/129‑1 (MQ=12)                                     cAGGAAAAAGTAAATCCTGGCTCTAAAATTCACGCCGCGGAGATGATGCTGCACCAGATGGGTTGGACTGAAGCGCCTGACCTGATTGTTAAAGGTAAGGAAGGGGCAATCACAGCAAAACACGTAAcg  >  2:261040/1‑128 (MQ=9)                                                                                                                      |                                                GAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACC  >  NC_000913/1196128‑1196292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑