| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | 100% | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 96.6% | 30.5 / ‑6.3 | 16 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/7); minor base G (1/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGA > NC_000913/124112‑124359 | gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGAGCAGGAAACAAAGGGCAAAGCGACAGTAATCCTTGCTCATACCGATAAAGGTTACGGCATGGGGGACGCGGCTGAAGGTAAAAATATCGCGCACTAGATTAACAAAAt > 2:20247/1‑143 (MQ=255) gaagaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCTTTAAAGGTTCCGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACa > 1:153649/1‑143 (MQ=255) gaaTATCTACTCTGCATTCAAGTAAGCGCAGGAATCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATgg < 1:16985/143‑1 (MQ=255) gcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATCTCCGCCCCCGtt > 2:11404/1‑143 (MQ=255) cAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCAGCGACCGttt > 2:288468/1‑143 (MQ=255) aGGAAACCAAAGGCAATGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc < 1:192040/143‑1 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGGTAAGAAGACGAACCTGGACGGAGTACGTCATAACTGCGACCGGTTCCAttggc > 2:23194/1‑139 (MQ=255) aaGGCAATGCGACAGTAATCCTTGCTCATAACATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 1:263921/143‑1 (MQ=255) aaGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCt < 1:93556/143‑1 (MQ=255) gACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAACCCTCGCGCACTAGGTTCAGAACATGAACCTGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGACGGTGTCTGATg > 1:155939/1‑143 (MQ=255) gTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGGCGCGACTGAAGGTAAAAACGTCGCGCACGAGGTGAAGAAACTGAACATGGACGGTGTCCGTCATATCCGCTACCGTTTACAAGGGCCGGTGTCGGATGCAGa > 2:116948/1‑143 (MQ=255) ccATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCg < 1:131804/143‑1 (MQ=255) ttAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTGTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACAGCCGTAc > 2:202320/1‑143 (MQ=255) gCATGGGCGACGCGGCTGACGGTAAAAACATCGCGCACTAGGGTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCCGATATCGAAAAACTGCCGTACATCACCTTCCCg > 2:285478/1‑143 (MQ=255) gggCGTCGCGGCTTAAGGTAAATACCTCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATGTAAAAACTGCCGTACATCACCTTCCCGGAAg < 2:91619/143‑1 (MQ=255) gCTGAAGGTAAACACATCTCGTATTAGGGTAAGAAAATGAACATGGACGGTGGGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAaga < 1:13513/143‑1 (MQ=255) | GATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGA > NC_000913/124112‑124359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |