| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 95.1% | 13.2 / ‑6.4 | 11 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (4/6); minor base A (1/0); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTA > NC_000913/2367982‑2368225 | ccgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATAAAAGCCCAGCTGGCAGCCGGTTACGAACTTAGCGCCAt > 1:149037/1‑143 (MQ=255) cgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTATCTCGTTATGGGATGTCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCAtt < 1:298067/143‑1 (MQ=255) cccGCTGTTTTGTTCAGCAAGTTATCCGGCCATCCAGCAAGGAAAATTAATAATGAAAACCGTCGTTTTTGTCTATTTCTTTTTTTGATTCTTCGGTATTGAAGCACTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTa < 1:3781/143‑1 (MQ=255) ccGCTATTTTGTTCAGCAAGTTAACGGGCCAACCAGCAAGGAAAATGAATAATGAAAAAAGTCGTTTTTGAATACCACGAAATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTAc > 2:89539/1‑143 (MQ=255) atGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATAAGCCGCAACTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCCCGTCt > 1:221196/1‑143 (MQ=255) ccTACCACGATATGGGATGCCTCGGTATTGAAGACCTGCTAGCCGCGGGTTAGGAAATTAGCGCCAGTTTTACCCATACTGATAATACCGGTGAAAAAGACTGTTATGGTTTGGTGGCTAGACTGGCGGCGGAGAGAGGCaac > 2:29708/1‑141 (MQ=255) gATATGGTATGGCTCGGTATTGAAGCCCTGCTGGGCGCCGGTTACGAAATTAGCGCCATTTTTACCCATTCTGTTAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTa < 2:23618/143‑1 (MQ=255) atatGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTTCGGAATTCGCGCCTTTTTTTTTCATGCTGATTTTCCTTTTGATAAAGATTTTTAAGGTTAGGCGGCACGGCTGGCGGCGGAAAGAGGCATTCCGGTTTAt < 1:40407/143‑1 (MQ=255) cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCTGGTGAAAAATTCTTTTATATTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATaa < 1:89539/143‑1 (MQ=255) cGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATACCGGCGCAATAATCTTTCATGCATGACGAAGTCGTGTGGCGGCGGCAAGAAGCATATCACGTTATGCGCCGGAGAACg > 2:214387/1‑143 (MQ=255) tATTCAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATATTCCCTGTGAAAAAGCCTTTTATTGTTAGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTa < 1:223336/143‑1 (MQ=255) | CCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTA > NC_000913/2367982‑2368225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |