| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,452,315 | C→T | 16.3% | A8T (GCC→ACC) | yfcS ← | putative fimbrial chaperone YfcS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,452,315 | 0 | C | T | 16.3% | 52.6 / 3.4 | 28 | A8T (GCC→ACC) | yfcS | putative fimbrial chaperone YfcS |
| Reads supporting (aligned to +/- strand): ref base C (13/9); new base T (2/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGAACAACCGG > NC_000913/2452174‑2452451 | gATCGTTTTTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCa < 1:298712/143‑1 (MQ=255) aTCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCag > 1:56919/1‑143 (MQ=255) cTGATTGATTTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGa < 2:136506/143‑1 (MQ=255) gattTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTc < 1:202086/143‑1 (MQ=255) tttGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCt < 2:244927/143‑1 (MQ=255) tAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAAACAGACATGGATCATTCCTTAATCACGTTATTg > 1:223574/1‑143 (MQ=255) atCAAACGTGTACGATCTGGCGTTACTGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAg < 1:19984/143‑1 (MQ=255) atCAAACGGGGACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAg > 2:231680/1‑143 (MQ=255) aaaCGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTAGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGAAGAGCAAAGTAAATCAGACATGGAACATTCCTAAATAACGTGATTGGTAAGcag > 2:18821/1‑141 (MQ=255) tACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTGAATCACGTTATTGGTTAGCTGAAACAGAAAAAGGGGGATCg > 2:163979/1‑143 (MQ=255) cAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGTCAGCGTCATAGCACCCAATTTTGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggt < 2:117159/143‑1 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGCGGACGGGTGGGTGTCAcc > 2:301941/1‑143 (MQ=255) gACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGTAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGtt > 1:257388/1‑143 (MQ=255) gACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGTAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGtt > 1:100161/1‑143 (MQ=255) tGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGCTCATTCCTCAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGGCCGCTGTTACGGCTg > 2:129869/1‑143 (MQ=255) cttAGCGCTAAGCATTTACGCCAGCGTCTTCTGACTCATTTTGGCAGATCAACGTAAATCAGACATGTATCATTCCTTACTCACGTTATTGGTTAGCTGAAACAGAAACTGGGGGTTCTTTGGGTGTCACCTGTTGCGTCTGc < 2:43072/141‑1 (MQ=255) gTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGCAACAGGGGGATCGGTGGGTGGCACCTGCTGCCTCTGca > 1:228507/1‑142 (MQ=255) gcgcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCt > 1:210307/1‑143 (MQ=255) cgcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACCGGGGGATCGGTGGGTGTCCCCTGTTGCGTCTGCTCCTg > 1:18040/1‑143 (MQ=255) tAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATCGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGCATCGGTGGGTGTCACCTGCTGCGTCTCCTCCTGCAt > 2:62984/1‑143 (MQ=255) aGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGa > 1:244200/1‑143 (MQ=255) cGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCgtt > 2:191320/1‑143 (MQ=255) atagtgTCATAGCACGCAATGTGGGAGAGCAAGGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTAt < 1:282043/138‑1 (MQ=255) gCACCCAATTTGGTAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGaa < 2:100161/143‑1 (MQ=255) gCACCCAATTTGGTAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGaa < 2:257388/143‑1 (MQ=255) ccAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACACGGGGATCTGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGaacaac < 2:150543/143‑1 (MQ=255) ccAAGTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTGATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGaacaac < 2:56919/143‑1 (MQ=255) aTTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTCCAACAGACATCGGCGGTTCTGTGGGTGTCACCTGTTGCATCTACAAATGAATAAAAGAAATTATGTCATAAGGAACAAACgg > 1:111679/1‑143 (MQ=255) | GATCGTTATTACGCAGCGTCACGCTGATTGATTTGTCGCTTTCATTAAATATCAAACGGGTACGATCTGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATGTCATCAGGAACAACCGG > NC_000913/2452174‑2452451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |