Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,634	A→C	100%	T108P (ACC→CCC)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,634	0	A	C	100.0%	38.2 / NA	16	T108P (ACC→CCC)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (4/12);  total (4/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041495‑3041770                                                                                                                                            |                                                                                                                                         ccATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGAATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCa                                                                                                                                       <  1:232119/143‑1 (MQ=255)   aTTGCGACGCGAAAGGTAAAATGTGGAGCAATATACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACTGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATc                                                                                                                                     <  2:87182/143‑1 (MQ=255)          cGCCAATGTTAATATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGCGAGTGAACCGCAGTGGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAg                                                                                                                              <  1:125795/143‑1 (MQ=255)            ccAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGCTCTGACAAAATATGCGGTATTCAATAAAGTGCCCATCACGCAAgac                                                                                                                            >  1:198492/1‑143 (MQ=255)                                        gcTGGTCCGCAACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCTTGCGTGAACCGCAGCTGAATGCTCGGATTAAATATGCGGTTTTCTCTTATTTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCg                                                                                                <  2:265127/142‑1 (MQ=255)                                                gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                        <  1:57317/143‑1 (MQ=255)                                                gcgACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg                                                                                        <  1:60414/143‑1 (MQ=255)                                                         aTGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGcgcgcc                                                                               >  2:254469/1‑143 (MQ=255)                                                              tttGCATGGATGGATCGGTGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTGAAGTGCCCATCGCGCCAGACGGCTAGTGTTTTCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt                                                                          <  2:164562/143‑1 (MQ=255)                                                                tGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTgg                                                                        <  1:224256/143‑1 (MQ=255)                                                                      gATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCCCTGGCACAtc                                                                  >  1:130469/1‑143 (MQ=255)                                                                            aCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTTTTTTCTTATGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTa                                                            <  1:126407/143‑1 (MQ=255)                                                                                 gcAGCGTGCGTGAACCGCAGCGGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa                                                       <  2:35976/143‑1 (MQ=255)                                                                                  cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTACAGTGCCCATCGCGCCAGACGACGAGCGCGTGCTGCTTGGCGTTGCCGGTTTTCCGGCGCGCGCCGCGCGGGCCAACCTCCTTCGCGCAc                                                      >  2:237753/1‑143 (MQ=255)                                                                                   gcctcgcgtgAACCGCGGCCGACTGAACTGCAAAACTATGCGGTACTCTCTAAAGTGCCCATCGCGCTAGACGCCGCGCGAGTGCTCCTTGGTGTTGCCGGTTTTCAGGCGCGCCCCGCGCTGTCAAATCTCTTTAGCGAACt                                                     <  2:305658/138‑1 (MQ=255)                                                                                                                                      aatgTGCCCACCGCGCCAGCAGACGAGCGTGTGCTGCCTGGTGCTGCCGGTTTTCCGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGATCTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATg  <  1:14129/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         CCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATG  >  NC_000913/3041495‑3041770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑