| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,240,359 | T→A | 23.6% | I12N (ATC→AAC) | psiE → | putative phosphate starvation‑inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,240,359 | 0 | T | A | 23.6% | 24.5 / 4.7 | 17 | I12N (ATC→AAC) | psiE | putative phosphate starvation‑inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (6/7); new base A (2/2); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GCGCGCTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTGGCTGATGTGCTGTTCGCGCCAGAACAAACCAGCAAA > NC_000913/4240221‑4240486 | gcgcgcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGCCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTcc > 1:184318/1‑143 (MQ=255) gcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGATAAACTccacgc < 1:224070/143‑3 (MQ=255) gcTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGATAAACTccacgc < 1:224143/143‑3 (MQ=255) tGAACAAAACATACACAAAAAATTTAGATCTCCGCCAGATTTTTGCGTTATACAGTAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACTTTACTCTCTCGTCCGCGCGTGTAGTTTATCTCCACCATTTTGCAGACCGTAc < 2:223285/143‑1 (MQ=255) cAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCaa > 1:283626/1‑143 (MQ=255) cacgAACAATATAGTTCTCCGGCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCGCTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCct < 2:298196/143‑1 (MQ=255) aaaaTATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCGCGCGTCCGCGCGGGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCtgt < 1:85929/143‑1 (MQ=255) tataTCTCCGTTACATTTTTGCTTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTCCTGTGCCTCg < 1:50352/140‑1 (MQ=255) aGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCaac > 2:81839/1‑142 (MQ=255) ggAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAg > 1:90367/1‑143 (MQ=255) gtcACTGTGAAGGAGGTACTTCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTg < 1:215686/141‑1 (MQ=255) tgtgAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCt < 2:184318/143‑1 (MQ=255) tgAAGGAGGTACTCCTATGACGTCCCTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTgg < 2:111734/143‑1 (MQ=255) aCTGCTATGACGTCACTCTCTCGTCCGAGAGTACAGTCGGAACCTAGTATTTTGCAGACCGTACTCAATCTTGTCCTTCTGGTCCGCCGCGAGATGTGGGGGGTCTGCCGTGGCAAAGAATCGGTGCATCTGGCTGATGTGcg > 2:105771/1‑142 (MQ=255) tGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCCAGGTACCGGTGTATTTGGCTGATGTGCttt > 1:140025/1‑141 (MQ=255) tcGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAGAGAAACGGTGCATCTGGCTGACGCGCTGTTCGCGCCAGAAcccaccg > 2:280425/1‑137 (MQ=255) aaatggTGGAGATAAACTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTCCCCCGTCCTTATTTTGACTGTCTTCATCAGCAAAGAAACGGTGCATCTGGATGATGTGCTGTTCGCGCCAGAACACACCAGCaaa > 1:236965/6‑143 (MQ=255) | GCGCGCTCCAAATCCAGGTTGAACAAAACATACACAAAAAATATAGATCTCCGTCACATTTTTGCGTTATACAGGAAGCTCGCCACTGTGAAGGAGGTACTGCTATGACGTCACTCTCTCGTCCGCGCGTGGAGTTTATCTCCACCATTTTGCAGACCGTACTCAATCTTGGCCTGCTGTGCCTCGGCCTGATTTTGGTTGTCTTCCTCGGCAAAGAAACGGTGCATCTGGCTGATGTGCTGTTCGCGCCAGAACAAACCAGCAAA > NC_000913/4240221‑4240486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |