| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 754,446 | A→G | 100% | L8P (CTC→CCC) | gltA ← | citrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 754,446 | 0 | A | G | 94.0% | 38.6 / ‑5.5 | 15 | L8P (CTC→CCC) | gltA | citrate synthase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (8/6); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAG > NC_000913/754311‑754579 | cAGGATGCGGTTGAAGCGAAGCCTCGGTCAAAGCTGACCACACCTTTCGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCCGCACATCCAGTCCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGc < 1:82504/143‑1 (MQ=255) gCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACAGCCAGTTCAACATCTGTATCCCCGTTCAGGGAGAGTCTTGCCTTTGt > 1:120029/1‑143 (MQ=255) ggatGGGTCAAAGGTGAACACACCTTTCGAACCGAGCGTACGGATATCAATAACATCTTGGCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg < 2:221662/140‑1 (MQ=255) acCTTGCGAACCGAGATCACGGATCTCAGTAACAGCTTGGCCCAGCGTGGCTTTCAGCACATCCAGTTCAATAGCTGTATCCACGTTGATGTTGGGTTTTGCTTTTGTATCAGCTATTTAAGGTCTCCTTAGCGCCTTATTGc < 2:52827/143‑1 (MQ=255) aaCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACt > 2:257630/1‑143 (MQ=255) tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGAATGCCGGAACTTAAATTTg > 1:277617/1‑143 (MQ=255) tcATCTTGACCCATCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTCTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg < 1:180564/142‑1 (MQ=255) aCATCTTGACCCGGTGTGCCTTGCAGCACATGCAGTGCAACGGCTGTTTCCCCACAAAGGGTGGGTGTTGCTTTGGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg < 1:125649/143‑1 (MQ=255) aGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACCTAAATTTACCTTCTCACAtaaccatg > 2:129021/1‑136 (MQ=255) tGCCTTTCAGCACAGCCAGTGCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAGCAGCCACCTAAGGTCCCCTTAGCGCCCAATTGCGTAAGACTGCCGGAACATAAATTTGCCTTCGCACATCAACCTCGCtt > 1:182105/1‑143 (MQ=255) cacaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttt > 2:67807/1‑143 (MQ=255) tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACATGGCTTTACCCGTTTTTTAt > 1:56348/1‑143 (MQ=255) aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTCAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCtaggcg > 2:144505/1‑138 (MQ=255) gTATCTCCGTGTAGGGTCGTTTTTGCTTTTGTATCAGCCCTGTCAGGTCTCCTTAGCGCCTTATTGCGTAAGACTTCTGGACCTTAAATTTGCCTTCCCACATCAACCTGGCTTTTCCCGTTTTTTATTTGGCTCGCCGctct < 2:120029/143‑1 (MQ=255) ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGCACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCAGTTTTTGATTTGGCTCGCCGCTCTATGaacgcg > 2:81894/1‑139 (MQ=255) | CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAG > NC_000913/754311‑754579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |