| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | 100% | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 100.0% | 19.3 / ‑6.7 | 12 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (6/4); minor base C (1/0); total (7/5) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760360‑760611 | atcgtcTGTAGGAGCTGGATAAACGAATCAGAACGGAGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 1:216657/138‑1 (MQ=255) ggAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCGGTCTCGCGTTGCCAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAATACCTCCCttcggcct > 1:92727/1‑137 (MQ=255) ctcgCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAACTGCAGTCTCGCGTTGCCAAAATATATGGCGATCGCCCGGCGATGACTGCCGGTGAGAAACTGTTAGACGAGCACGCGGCGGACAACCTCGCTGACGCAACgac > 2:224975/4‑141 (MQ=255) cATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACTTCGCTTACGCCGTGCTGGTTGATGAAg < 2:64607/143‑1 (MQ=255) ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCggcccg > 2:277662/1‑139 (MQ=255) gCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACCCCGGTCGCgggag > 1:304734/1‑140 (MQ=255) ttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTACGGTCACGGTACCTTCTTcc > 1:187021/1‑143 (MQ=255) aTGCCGATCGCCAGGCGATGTCTGTCGGTGATAATCTGTTCTATTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:75206/143‑1 (MQ=255) cGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGTGTGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgc < 1:237406/143‑1 (MQ=255) cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGTCCTTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:221739/1‑143 (MQ=255) gCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt < 1:234556/143‑1 (MQ=255) gaAAATGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGGCGCGGTACCGTCTTCCACCGCCACGCGGTGATCGACCACCAGTcaaccg > 2:242453/1‑138 (MQ=255) | AGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760360‑760611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |