| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,499,945 | C→T | 16.8% | A169T (GCA→ACA) | ydcK ← | putative enzyme YdcK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,499,945 | 0 | C | T | 16.8% | 41.3 / 3.2 | 24 | A169T (GCA→ACA) | ydcK | putative enzyme YdcK |
| Reads supporting (aligned to +/- strand): ref base C (6/14); new base T (2/2); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
GTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCC > NC_000913/1499811‑1500085 | gTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGa < 2:208682/143‑1 (MQ=255) tttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAgg > 1:229301/1‑143 (MQ=255) aTCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTGAATAAAAGCATACCGGACGACGGCAGCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAAccc < 1:249957/143‑1 (MQ=255) cGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAATCTTCGGCGCGATGTTCAATAAAAGCATAACGGAGGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCtt < 1:211152/143‑1 (MQ=255) aGCCAGACATTGTTCCCTTCATTACCTTCAATACTGGCAAAACCAAAACCTGCGGCGCGATGTTCAATAACAGCATACCGGACGACGGCATCGCCATAAAGTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGcgcg < 2:267538/143‑1 (MQ=255) aGACATTGTTCCCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGCTCa > 1:134989/1‑143 (MQ=255) tCATTACCTTCAACACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAg < 1:214456/143‑1 (MQ=255) aTTACCTTCAATACTGGCAAAGTCAAAAACTTCGGCGCGATGGTCAATAAAAGCAAACCGGACGACGGCGTCGCCATAAATGTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGa < 1:93367/143‑1 (MQ=255) ttACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACACTTGCGCGCTCATAAATTTGGAGCAggcg > 2:193100/1‑141 (MQ=255) cTTCAATCCTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCGTCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGa < 2:179942/143‑1 (MQ=255) ttCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAGAGCATACCGGACGACGGCATCGCCATAAATTTGTGTCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGAt > 1:168884/1‑143 (MQ=255) ttCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGTCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGAt > 1:22894/1‑143 (MQ=255) aaaaTCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATCCGCGAGGCGCTCACCCTTGCGCGATCATAAATTTGGAACAGGAGTTGATGGTCGGGCGTTa > 1:3248/1‑143 (MQ=255) aTGTTCAATAAGAGCATACCGGACGACGGCATCGCCATAAATTTGTGTCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCa < 2:168884/143‑1 (MQ=255) aTGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGTCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCa < 2:22894/143‑1 (MQ=255) cATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAAGTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCa < 1:303990/143‑1 (MQ=255) aCCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCAt > 2:202441/1‑127 (MQ=255) aCCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCAt < 1:202441/127‑1 (MQ=255) aTCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAGTGTTGGTCAATTAGGGCGTGACCaa > 2:219482/1‑143 (MQ=255) aTAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAAGTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTc < 1:178548/143‑1 (MQ=255) aagacggtgCCGGGTGCAGCATACGTGAGCCGCTAACCCGTGCGCGATCATAAATGTGGAGCAGGAGTGGATGGGCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTa < 1:234834/137‑1 (MQ=255) aaaTTTTTGCCTGGTGGACAAGACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTa < 2:93164/143‑1 (MQ=255) gtgCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGc < 1:200016/143‑1 (MQ=255) tgCGTGGTGGACAATACGCGAGGCGCTAACCTTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGcc < 1:273263/143‑1 (MQ=255) | GTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAGGCGCTAACCCTTGCGCGATCATAAATTTGGAGCAGGAGTTGATGGTCGGGCGTTAAGCCTTGTGCGGCGACGATCATAGAATGTTGATCAATTAAGGCGTGACCAAATATTCTACATTGCC > NC_000913/1499811‑1500085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |