| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 124,241 | C→T | Q409* (CAG→TAG) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 124,241 | 0 | C | T | 94.8% | 143.2 / NA | 60 | Q409* (CAG→TAG) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (19/36); total (22/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCA > NC_000913/124101‑124380 | gtggcgTTCACGATCCGAAGAAAATCTACTCTGCATTCAAGAAAGCGCAGGAATCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAg < 2:207623/143‑1 (MQ=255) ggtggtAACGACCCGAATAAAATCTGCGCTGCACTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGt < 2:400293/143‑1 (MQ=255) tgggCACGATCCGAAGAAAATCTACGCTGCGTGGCAGAAAGCGCAGGACACCAAACGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTa < 2:279855/143‑1 (MQ=255) tCACGATCCGAAGAAAATCTACTCTGCATTCAAGAAAGCGCAAGAAACCAAAGGCAAAGCGACAGTAATGCCTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGa < 2:857772/143‑1 (MQ=255) tCACGAGCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGa < 2:796422/143‑1 (MQ=255) cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCGAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaa > 1:590927/1‑143 (MQ=255) aggagccGAGGAAAGTCTACGCGGGAGTCAAGAAAGCGGAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCAGGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaaa < 1:808013/138‑1 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAGGGTAAAACCATCGCGCACCAGGTTCAGAAAAt > 1:351650/1‑143 (MQ=255) gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 1:499674/143‑1 (MQ=255) gATCCGAAGAAAATCTACGCCGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt < 1:187638/143‑1 (MQ=255) aTCCGAAGAAAATCTACGCTGCATTCCAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATg > 1:1028690/1‑143 (MQ=255) gcaaataaaaTCTACGCTGCATACAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGaa < 1:1120796/137‑1 (MQ=255) agaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATg < 1:697712/143‑1 (MQ=255) gaaAATCTACGCTGCATGCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGATACGGCAGGGGCGACGCGGCTGAAGGTAAAAACATCGCGCAATAGGGAATGAAAGTGAACATgg > 2:177660/1‑143 (MQ=255) aaaTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGAc > 1:116724/1‑143 (MQ=255) ctCTACTCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACgg < 2:1053045/142‑1 (MQ=255) ctccGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTGATACCAATAAAGGTTACGGCATGGGCGCCGCCGCTGAAGGTCAAAACATCGCGAACCAGGTTACGACAATGAACATGGACgacg > 2:712392/4‑140 (MQ=255) tACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtgt < 1:250749/143‑1 (MQ=255) ttCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCCTTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGCTAAGAAAATGAGCATGGAAGGTGTGCGTCatat > 2:420118/1‑143 (MQ=255) tCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATc > 1:841890/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg > 1:673535/1‑143 (MQ=255) aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAAAATAGTGCATTGTGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcg > 1:519709/1‑143 (MQ=255) gAAAGCGCAGGCAACCAAAGGCAAAGCGGCAGTAATCCTAGCTCATACCCTTAAAGGTTACGGCATGGGCGACTCGGCCGACGGTAACAACATCGAGGACTAGGTTGAGCAAATCAACATGGAGGGGGTGAGGCatatgagag > 2:798267/1‑138 (MQ=255) gAAAGCGCAGCGAACCAAAGGCGAAGCGACAGTAATCCTTGCTCATACCCCTAAATGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCcgcg < 2:1045426/143‑1 (MQ=255) cAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGttt < 2:895245/143‑1 (MQ=255) ggAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCa < 2:18760/143‑1 (MQ=255) gAACCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAATATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa < 1:603943/143‑1 (MQ=255) gAAAGCAAAGGCAAAGCGACAGTCATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCaa < 1:800288/143‑1 (MQ=255) aaCCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATAAACATGGACGGTGTGCGTCATATCCGCGACCGTGTCCAtg > 2:656008/1‑143 (MQ=255) cAAAGGCAAAGCGACGGGAATCCTGGCGCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGc < 1:875954/143‑1 (MQ=255) cAAAGGCAAAGCGACAGTAATCCTTGATCATACCATTAAAGGTTACGGCATGGGCGACGCGGATGAAGGTAAAACGATTGCGCACTAGGTTAAGGAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTGAATGTGc > 1:830236/1‑143 (MQ=255) aaGGCAAAGCGACAGTAATCCTCGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCg < 1:691681/143‑1 (MQ=255) aaaGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTACGTCATAACCCCGACCGTTTCAAAGTACCAGTGTc > 1:2867/1‑143 (MQ=255) aGCGACAGTAATCCTTGCTCATACTATTTATGGTTACGGCATGTACAACGCGGCTGAAGGTAAAAACACCGAGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTg < 1:1124009/143‑1 (MQ=255) aGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTg < 1:452419/143‑1 (MQ=255) aGTAATCCGTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAg < 1:162147/143‑1 (MQ=255) tAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGat < 2:590927/143‑1 (MQ=255) aTCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGatat > 1:245494/1‑143 (MQ=255) cTTGCTCATACCATTAAAGGTTAGGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATTGa < 2:212998/143‑1 (MQ=255) cTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGa > 1:808858/1‑143 (MQ=255) cTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAACAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGa > 1:806557/1‑143 (MQ=255) ttgcgtaTACCATTTAAACTTACGGCATGTTCGACTCGGCTGATGGTAAAAACATCTCGCACTAGCTTAACAAAATTAACATGGACGTTGTGCGTCATATCCTCTACCGTTTCAATGTGCCGTTGTCTGATGCAGATATCGaa < 2:5686/137‑1 (MQ=255) ccATGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCg < 1:557551/143‑1 (MQ=255) cATTAAAGGTTACGGCATGGGCGACGCGGCTGGAGGAAAAAACATCGCGCACTGGGGTAAAGGAATGAACAGGGACGGTGTGCGTCATACCCGCGAGCGTTTCAATGTGCCGGTGACTAATGCAGGGATGGAAAAAATGCAGt > 2:949424/1‑143 (MQ=255) cATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCgg < 1:372895/143‑1 (MQ=255) tGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTGTCGATGTGACGGTGTCTGCTGCAGATATCGAAAAACTGCCGTACATGACCTTCCCGGaa > 1:309140/1‑143 (MQ=255) gcgGCTGAAGGAAAAAACATCGCACACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGa < 1:1060888/143‑1 (MQ=255) cgGCTGAAGGTAAGAACATCGCGTACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa < 2:865554/143‑1 (MQ=255) cgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAACTGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGaa < 1:688466/143‑1 (MQ=255) cTGAAGGTAAAACCATCGCGCACTAGGTTAGGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGAGGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAagag < 2:1091714/143‑1 (MQ=255) taaaGCTAAAAACATCGCGCACGAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGc < 1:1027696/141‑1 (MQ=255) aGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGTGGGTGTGTGTCAGATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa < 1:1075880/143‑1 (MQ=255) aaaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAt < 1:959684/143‑1 (MQ=255) aaaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTAt > 1:634196/1‑143 (MQ=255) aaaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATc > 1:118805/1‑143 (MQ=255) aaCCTCGCGCAGTAGGCTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAACGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCt < 1:316756/143‑1 (MQ=255) cATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTATGAAGAGCCTACCTATCTGc > 2:318372/1‑143 (MQ=255) agcgcgCACTTGGTGAAGAGAATGAACAGGGGCGGGGCGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGGTATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCa < 2:665106/141‑1 (MQ=255) cACTAGGTTGAGACAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAACAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCa < 2:563908/143‑1 (MQ=255) cACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCa < 2:430437/143‑1 (MQ=255) | GTGGTGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCA > NC_000913/124101‑124380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |