Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	95.4%	172.5 / NA	66	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (26/36);  minor base G (1/2);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696334‑696607                                                                                                                                         |                                                                                                                                          gCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATc                                                                                                                                     <  2:237281/143‑1 (MQ=255)   atgcGACATTGAAGGATAAGACGTGGCAACAGCGAATCCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  1:404600/140‑1 (MQ=38)   aTCCGACATTGAAGGATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   <  1:322687/143‑1 (MQ=255)    tCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCgg                                                                                                                                  >  1:856193/1‑143 (MQ=255)      cGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTg                                                                                                                                >  1:486439/1‑143 (MQ=255)       gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGAATTAGAATCCGCTGc                                                                                                                               >  1:505347/1‑143 (MQ=255)       gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAATCCGGTGc                                                                                                                               >  2:823680/1‑143 (MQ=255)          aTTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                            >  1:664821/1‑143 (MQ=255)             gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                         <  1:525613/143‑1 (MQ=255)             gAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAcc                                                                                                                         >  1:166810/1‑143 (MQ=255)                   tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:523746/143‑1 (MQ=255)                   tAAGACGTGCCAACATCGCATTCGACATTGAAAGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTTGAACCTCGGAATGCCGGAATTAGAAGCCGGTGCCTTACCGCTTgg                                                                                                                   <  1:639151/143‑1 (MQ=37)                   gaggtggtgtCAAGATGGCGTGCGTCATGGCATGCCCGCCGAAAAGCAAAGAGCTCGCCGAAGCGAGCGTTTTTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:355253/137‑1 (MQ=18)                    gaGACGTGGCAAGATCGGTTTCTATATTGAATTAACGCAGAAAACCAAAAAGCTCGCCGAAGAGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGTAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                  <  2:257808/142‑1 (MQ=21)                      gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCATGGCGa                                                                                                                >  2:160600/1‑143 (MQ=255)                          tgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcc                                                                                                            <  1:197531/143‑1 (MQ=255)                              aaCATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCGCGTGGCGATACCCCac                                                                                                        >  2:844021/1‑142 (MQ=255)                                  tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTCGAGTCCGGTGCCTTACCGCTTGGCGATACGCCAACTgg                                                                                                    >  1:308759/1‑143 (MQ=255)                                  tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                    <  2:322214/143‑1 (MQ=255)                                      aTTCGACATTGAATGAACGCAGAACAGCCAACAGCTCGTGGGGGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                <  1:472091/143‑1 (MQ=21)                                      aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                                                                                                <  1:888421/143‑1 (MQ=255)                                      aTTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAATAGAATCCGGTGCCTTACCGCTTGGCGCAACCCCAACAGGgcgc                                                                                                >  2:875228/1‑143 (MQ=25)                                          gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTAAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAACTGGGTGCACtt                                                                                            >  2:532824/1‑143 (MQ=255)                                             aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTGTTGTTGCTCAATAACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                         <  1:1128037/143‑1 (MQ=21)                                             aTTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGGGGTGCACTTaaa                                                                                         >  1:694252/1‑141 (MQ=255)                                              ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  1:539172/143‑1 (MQ=255)                                               tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAAGGTGGCTGGGGTACGATGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                       <  2:916837/143‑1 (MQ=255)                                                gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg                                                                                      >  1:356587/1‑143 (MQ=255)                                                  atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                                                                                    >  1:945416/1‑143 (MQ=255)                                                  atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAGGTGGACTTGCacgcgc                                                                                    >  2:141183/1‑138 (MQ=21)                                                     acgggagaagaGCAAAAAGCGCGCCGAAGCGAGGTTTTTTTCTGTCGCTAAAGTACGTGGTCTCAAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 <  1:1012553/138‑1 (MQ=18)                                                     aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGc                                                                                 >  1:25655/1‑143 (MQ=255)                                                       cGCAGAGAGGCAAGAAGCTCGCCGAGGCGGGCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                               <  2:773244/143‑1 (MQ=21)                                                       cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt                                                                               >  1:112284/1‑143 (MQ=255)                                                          aGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCtt                                                                            <  1:778831/143‑1 (MQ=255)                                                           gAAATGTAAAAAGCTCGCGGAGGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                           <  2:540350/143‑1 (MQ=21)                                                           gAAAAGCAAAAAGCGAGGCGAAGCGAGCTGTTGTAAGGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                           <  1:67213/143‑1 (MQ=21)                                                            aaaaGCAAAAAGCTCGCCGAAGCGAGCGTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa                                                                          <  1:245965/143‑1 (MQ=255)                                                                   aaaaGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAtt                                                                   >  1:700086/1‑143 (MQ=255)                                                                    aaggCTCGCCGAGGCGGTCTTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGGATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTg                                                                  <  2:256368/140‑1 (MQ=21)                                                                          cGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACGTCGGAATGCCGGAATTAAAAACCAATGCGTTTGGCCAAAGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                            <  1:477600/143‑1 (MQ=25)                                                                           gCCGGATCGAGCTTTTTTAATGTGGTTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACTAGGTACGCGTCTTGAATAAATTGGCTtggg                                                           <  2:427496/143‑1 (MQ=21)                                                                            ccGAAGCGAGCTTTTGTAATGTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGt                                                          <  1:721343/143‑1 (MQ=255)                                                                                   gAGCTTTTTTAATGTGGCGGGGGTACGAGGATTCGAACCTGGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                   <  2:987786/143‑1 (MQ=255)                                                                                    aGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                  >  1:787006/1‑143 (MQ=255)                                                                                          gttAGTGCGGCTGGGGCGCGAGGACGCGAACCTCGGACTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGCGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAAc                                            <  2:1011166/142‑1 (MQ=25)                                                                                           ttAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTCCGAGGATTCGAAcc                                           >  1:845536/1‑143 (MQ=255)                                                                                             aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGACTAAATTGGCTGGGGTACGAGGATTCGAACGTc                                         >  1:122755/1‑143 (MQ=255)                                                                                             aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  1:530599/143‑1 (MQ=255)                                                                                             aaTGTGGCTGGGGGAGGGGGATTTGAACCTCGGAATGCCGGAATTAGGATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTc                                         <  2:862420/143‑1 (MQ=37)                                                                                              aTGTGGCTTGGGCACGAGGGTTCGAACGTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCg                                        <  2:277385/143‑1 (MQ=37)                                                                                                 tgGCTGGGGTACGAGAATGCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGaa                                     <  1:875228/143‑1 (MQ=255)                                                                                                  ggCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGTCTCGACTAAATTGTCTGGGGTGGGGGGATACGAACGTCggcgt                                    >  1:486994/1‑140 (MQ=18)                                                                                                      ggggTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                >  1:563180/1‑143 (MQ=255)                                                                                                         gTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGaa                             <  1:922154/143‑1 (MQ=255)                                                                                                            ggaggagTCGAGCCTCGGAAGGCCGGAATGAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:1044240/142‑1 (MQ=255)                                                                                                            cgcggcttCGAACCTCGGAATGGCGGAATTAGTATCCGGCGCCTTCCCGATTGGCGATACCCCAACTGGGTGAAATTACGAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  2:856193/137‑1 (MQ=37)                                                                                                            cGAGGATTCGAACCTCGGAACGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:59808/143‑1 (MQ=255)                                                                                                             gAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGTCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATCAg                         >  1:932536/1‑143 (MQ=255)                                                                                                                gATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGACCCTCGGCATGCCGGAGTCCGAAt                      >  1:735704/1‑143 (MQ=37)                                                                                                                    cGAACCGCGGAATGGCGGGACTAGACACCGGCGCATTACGGCTTGGCGGTAACCCAACAGGGCGCAATTTCGAGGTAGGCGGCGTGAGTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                  <  2:184472/143‑1 (MQ=12)                                                                                                                         cTCGGAATGCCGGAATGAGAATCCGGCGCCTTGCCGCTTGGCGATACCCCAACTGGGTGCACGTACAAGGTAAGCGTCTTGAATAAACTGGCTGGGGTCCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt             <  1:586032/143‑1 (MQ=37)                                                                                                                                 gCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTCCGCGTCTTGAATAAATTGGCTGCGGTACGAGGATTCGAACCTCGGAATGCCGGAATGAGAACCCGGTGCCTAACCGCtt     >  2:1009525/1‑143 (MQ=37)                                                                                                                                   cGGAATTAGAATCCGTTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTTTTTAATTTGGTTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg   <  1:85421/143‑1 (MQ=21)                                                                                                                                   cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg   >  1:356747/1‑143 (MQ=255)                                                                                                                                    ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGCGGATTCGAACCTCGGAATGCCGGAACCAGAATCCGGTGCCTTACCGCCTGGc  >  2:769575/1‑143 (MQ=37)                                                                                                                                         |                                                                                                                                          GCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696334‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑