| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,380 | 1 | . | C | 95.7% | 122.4 / ‑1.0 | 46 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (22/21); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913 | 4,296,380 | 2 | . | G | 95.7% | 131.0 / ‑1.2 | 46 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (22/22); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAG > NC_000913/4296302‑4296513 || tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCTGCGTCTTGTCAGGCCTATCTTAACCg > 1:1070409/1‑143 (MQ=11) cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg > 2:656715/1‑143 (MQ=255) cgcgCCTTATCAGGCCGACGCCAGACAGCGCGATAGCCTGATTCAGCGTGAGGTGGTAGGCCGGTTAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 2:199754/143‑1 (MQ=11) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt < 1:373473/143‑1 (MQ=255) cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt < 1:1068933/143‑1 (MQ=255) aGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc < 1:1006592/143‑1 (MQ=255) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc > 1:963275/1‑143 (MQ=255) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCaaa > 2:509889/1‑143 (MQ=255) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa < 2:510313/143‑1 (MQ=255) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCAGGCCTATATTAACAGTTGGTTAATTTTTCaaa > 1:221695/1‑143 (MQ=14) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTGTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAct > 2:39577/1‑143 (MQ=255) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCTAACCGTTGGTCACTCTTTCCAActct > 2:360589/1‑143 (MQ=11) gCCAGACAGCGCAATAGCCTGATCGAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGTTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 1:316834/143‑1 (MQ=17) ccATACAGCGCAATAGCCTGTTTTAGCGTGTTTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc < 2:370476/143‑1 (MQ=17) ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc < 2:102013/143‑1 (MQ=255) cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca > 2:261211/1‑143 (MQ=255) cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca > 2:261047/1‑143 (MQ=255) cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc < 1:23438/143‑1 (MQ=255) cAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc < 2:853524/143‑1 (MQ=255) agtgcAATAGCCTGATTTAGCGTGATTTTGGAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc < 1:192441/140‑1 (MQ=17) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGTTAATTTTTCCACCTCTTGACGccc > 1:542432/1‑143 (MQ=14) gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACAcccc > 1:349892/1‑142 (MQ=255) ggcAATGGCCTGATTTAGCGTGATTTTGTGGGTCGGGTACGGCG‑T‑TTTCG‑‑CCGCATCCGACATACATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGTCTTTGCCGTTGGTTAATTTTTCAAACTCTTCACACCCgg < 2:20855/142‑1 (MQ=17) ctggCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc < 2:285828/140‑1 (MQ=18) aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc < 1:35271/143‑1 (MQ=255) tggtcTGCTTTAGCGTGATTTCTTTGGTCGGATAGGGCGCTCTT‑‑C‑‑CGGCATCCGACATCAACGACTGATGCGACGCTTGACGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATGTTTCAAACTCTTCACACCCGGTATCa < 2:1078992/139‑1 (MQ=18) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAAc > 2:35409/1‑143 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCt > 2:1025826/1‑143 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCAGGCATATCTTAACCGTTGGTTAATTCTTCAAACTCTTCACACCCGGTATCaagacct > 2:923422/1‑138 (MQ=18) tttAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:922630/143‑1 (MQ=255) tttAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTc < 1:1040317/143‑1 (MQ=255) ttAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAACCCCTTcc > 2:468336/1‑143 (MQ=18) gCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa > 2:647862/1‑143 (MQ=255) tGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg > 2:83779/1‑143 (MQ=255) gATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCCAACCCTTccgtccatc > 1:976213/1‑136 (MQ=18) ttttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTCAACCGCTGGTTAATTCTTCAAACTCTTCACACCCGCTATCAAACCCCTCCATCCAGCTc > 2:306157/1‑143 (MQ=18) tttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCCCACCCGGTATCAAACCCTTCCATCCAGCTCa > 1:29745/1‑143 (MQ=21) tttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 1:261211/143‑1 (MQ=255) tttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 1:261047/143‑1 (MQ=255) tttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 1:656715/143‑1 (MQ=255) tttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTATTCACACCCGGCAACAAACCCGTGCATACCGCTCa > 2:1000458/1‑143 (MQ=18) ttGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAg < 1:218857/143‑1 (MQ=255) tGTAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgg < 1:899962/143‑1 (MQ=255) tAGGTCGGATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt < 1:1036724/143‑1 (MQ=255) ggATAAGGCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa < 2:782493/143‑1 (MQ=255) ggCG‑T‑TT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGCTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTCCAg > 2:876915/1‑143 (MQ=37) || TAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCG‑T‑TT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAG > NC_000913/4296302‑4296513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |