Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	87,881	A→G	8.6%	intergenic (+33/‑147)	ilvH → / → cra	acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit/DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	87,881	0	A	G	8.6%	153.6 / 5.6	65	intergenic (+33/‑147)	ilvH/cra	acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit/DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base G (3/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-02

GGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGG  >  NC_000913/87741‑88021                                                                                                                                             |                                                                                                                                               ggTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGCGGTTGATCTCAATGCGCAATTTACAGCCCAACTTg                                                                                                                                              >  1:112522/1‑143 (MQ=255)  gTAAGCTTGATGCTTTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAACTCATAAGATGTGAATGATCTCAATGCGCAATTTACAGCCCAACATGt                                                                                                                                             <  1:479338/143‑1 (MQ=255)    taGCGTGCTGCATTTTTAGCAGCGATTGGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCa                                                                                                                                           <  2:148123/142‑1 (MQ=255)        ttGAGGCGTGTTTGGCATCGATTCGCGAGGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGtt                                                                                                                                       <  2:434508/143‑1 (MQ=255)           aTGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAGTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTggg                                                                                                                                    >  2:909176/1‑143 (MQ=255)           aTGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTggg                                                                                                                                    <  1:954188/143‑1 (MQ=255)           aTGCATTGTTAGCATCGACTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCGCTGGTGTGGTCGGACTTTCGCGCGGCGATACAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTggg                                                                                                                                    <  2:560379/143‑1 (MQ=255)            tGCCTTTTTAGCATCGATGCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGc                                                                                                                                   <  1:475784/143‑1 (MQ=255)                   ttAGCCTCGATTCGCGATATGGCGAAAATTGTGGAGGTGGCTCGCTCTTGTGTGGTCGGACTTTCGCGCGACGATAAAATAATGCGTTGAGAATTATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑ttttttt                                                                                                                           <  1:486482/143‑1 (MQ=255)                   tgggggtCGAGTCGGGGTGTGGCGAATATTGGGGAGGTTGCGCGCTCTGGTGGGGTCGGACTTTCGCGGGGTGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑ttttttt                                                                                                                           <  2:201231/137‑1 (MQ=255)                        aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATCATGCTTTGAGAATGATCTGCATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTGTTTTGCGaa                                                                                                                      >  1:704979/1‑143 (MQ=255)                         tCGATTCGCGATGTGGCGAAAGTTGTGGAGGGTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGaaa                                                                                                                     <  2:1071933/143‑1 (MQ=255)                         tCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGTGCAATTTACAGGCGGACGTGTGATGTGGGGC‑TTTTTTTGCGcaa                                                                                                                     >  1:153064/1‑143 (MQ=255)                          cGATTCGTGATGTGGCGAAAATTGTGGAGGTTGCTCGCTATGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAAt                                                                                                                    <  1:810066/143‑1 (MQ=255)                              tCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGGGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGt                                                                                                                >  2:912941/1‑143 (MQ=255)                               cggtaTGTTGCTACAATTGTGGAGGTTGCTCGCTCTTGTGTCGTTGGACTTTCCCGCATCGATAATATAATGCGTTGAGAATGATCTCAATTCGCAATTTACATCCCATCATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTg                                                                                                               <  2:27829/139‑1 (MQ=255)                               cgcgATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGAGTTTGGAGCACCAATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTg                                                                                                               <  1:519471/143‑1 (MQ=255)                                tggATGTGGCGAAAATTGTGGAGGTTGCTCGCTCGGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTgg                                                                                                              <  2:43507/141‑1 (MQ=255)                                   attgggCTAAAATTGTGGATGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCTGCGATAAAATGATGTGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGaa                                                                                                           <  2:917882/139‑1 (MQ=255)                                        gCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCACTTTACACCCCAACATCTCACCTTGGGC‑TTTTTTTGCGAAACCAGTGGGGCCCTgg                                                                                                      >  2:203780/1‑143 (MQ=255)                                          gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCCGTGGGAACATGGaa                                                                                                    >  2:276577/1‑143 (MQ=255)                                               tgggtgAGGTTGCGCGCTGTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑aaaa                                                                                              <  2:768006/138‑1 (MQ=255)                                               gtgtgGAGGTTGCTCGCTGTGGTGTGGTCGGACGGTCGCGCGGCGATAAAATAAGGCGGTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑aaaa                                                                                              <  1:226538/142‑1 (MQ=255)                                                       gTTGCCAGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGc                                                                                      <  1:379944/143‑1 (MQ=255)                                                        gtgccggctcGGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAGTTTACAGCGTAGCATGTCACGTTGGGG‑TTTTTTTGCGTAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGcc                                                                                     <  1:1050680/137‑1 (MQ=255)                                                         ttctcgttcTGGTGGGGTCGGACTTTGGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACCGGCCAACATGTCACGTTGGGC‑TATTTTTGCTAAAGCAGTAGGAACCTGGAAT‑AAAAGCAGTTGCCg                                                                                    <  2:845311/141‑1 (MQ=255)                                                          gcgcgctcGGGTGTGCTCGGATTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAGTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGCGGGAACCTGGAAT‑AAAAGCAGTTGCCGc                                                                                   <  2:140800/140‑1 (MQ=255)                                                           ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCa                                                                                  <  1:897298/143‑1 (MQ=255)                                                                tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGTAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                             >  1:226212/1‑143 (MQ=255)                                                                tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                             >  1:223925/1‑143 (MQ=255)                                                                tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGCC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑ACAAGCAGTTGCCGCAGTTaa                                                                             >  2:698267/1‑143 (MQ=255)                                                                tctGGTGTGGTCGGACTTTCGCGCGGCGAGATGATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                             >  2:990405/1‑143 (MQ=255)                                                                 ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAt                                                                            <  2:876460/143‑1 (MQ=255)                                                                   ggTGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAttt                                                                          <  1:221324/143‑1 (MQ=255)                                                                       tgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                      >  1:314680/1‑143 (MQ=255)                                                                         ggcGGACTTTCGCGCGGCGATAAAATAAAGCGATGAGAATGAGCACAATGCGCAAGTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATGTTCTgcg                                                                    >  2:455965/3‑143 (MQ=255)                                                                          tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCAAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCACTGGGAACCTGGAAT‑AAAAGCAATTGCTGCAGTTAATTTTCTgcgc                                                                   >  2:295819/1‑143 (MQ=255)                                                                           cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCCACATGTCCCGTTGGGC‑TTTGTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCGGCAGTTAATTTTCTGCGCt                                                                  >  2:130585/1‑143 (MQ=255)                                                                               cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                                                              <  1:745433/143‑1 (MQ=255)                                                                                 ttCGCGCGGCGATAAAATAATGCGTTGAGAATGTTATCAATGCGCGATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCAGGAGTTAATTTTCTACGCTAAGATg                                                            >  2:1078576/1‑143 (MQ=255)                                                                                  tCGCGCGGCGATAAAATAATACGTTGTGAGTGATCTCAATGCGCAATTGACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                           <  1:417399/143‑1 (MQ=255)                                                                                      gcggccATAAAATAATGCGTTGAGAATGATCCCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAAt                                                       <  1:953094/143‑1 (MQ=255)                                                                                        ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑‑TTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGCTGTTAATGaa                                                    >  1:649770/1‑143 (MQ=255)                                                                                        agcgATAAAATAATGCGTTCAGAAGGATCTCAATGCGCAATTGACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGa                                                     <  1:563934/142‑1 (MQ=255)                                                                                           gttAAAATAATGCGTTGGGGATGATCTCAAGGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt                                                  <  2:158773/141‑1 (MQ=255)                                                                                           gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGTGGCCATTAATTTTCTGCGCTTAGATGTTAATGAAtt                                                  <  2:1110356/143‑1 (MQ=255)                                                                                             tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTa                                                >  2:214844/1‑143 (MQ=255)                                                                                                aatgatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAcc                                             <  1:409094/143‑1 (MQ=255)                                                                                                         ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGt                                    >  1:1032236/1‑143 (MQ=255)                                                                                                         ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCAATACCAGt                                    >  1:143236/1‑143 (MQ=255)                                                                                                         ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCGTAGATGTTGATGAATTTAACCTATACCAGt                                    >  1:876393/1‑143 (MQ=255)                                                                                                          tGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAC‑AAAACCAGTTGCCGCCATTAATTTTCAGCGCTTCGATATCAATTAATGTAACCCTTACCAATa                                   >  2:849392/1‑143 (MQ=255)                                                                                                           gagaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTAc                                  >  2:1112004/1‑143 (MQ=255)                                                                                                                  aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCACTACAATGGcc                           >  2:950370/1‑142 (MQ=255)                                                                                                                     tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTAt                         <  2:212072/143‑1 (MQ=255)                                                                                                                       aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCCCTTAGATGTTAATGAATTTAACCCATACCAGTACAAGTGCAATGGt                      >  2:51057/1‑143 (MQ=255)                                                                                                                         tGCGCAATTTACAGCCGAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTAACCGCAGGTATTGTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttt                    <  2:187628/143‑1 (MQ=255)                                                                                                                             cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTa                 >  1:99743/1‑143 (MQ=255)                                                                                                                                tttACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGGCGGAACGTTGAGA‑ATGATGAGTTGCCGCAGTGAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAtt             <  1:258077/143‑1 (MQ=255)                                                                                                                                tggagaGCCGAAGATGTAACGTTGGGC‑TTGTTCTGAGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTGAATTTGCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAtt             <  2:769774/138‑1 (MQ=255)                                                                                                                                   aCAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTa          <  1:264877/143‑1 (MQ=255)                                                                                                                                     aGCCCAACGTGACATGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAGTTTCTGCGCTGAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg        >  1:685111/1‑143 (MQ=255)                                                                                                                                     aGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGTAT‑AAAAGCAGATACCGCAGTTAATTTTATGAGATTAGATGATAATGAATTTAACCCATACTAGTACAATGGCTATGGTATTTACATTTTACg        >  1:661123/1‑143 (MQ=255)                                                                                                                                       cccAACGTGACATGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCa      >  2:881198/1‑143 (MQ=255)                                                                                                                                           aCATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg  >  1:541758/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                               GGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGG  >  NC_000913/87741‑88021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑