Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 464,356 A→T 6.2% intergenic (+48/‑46) ybaV → / → fadM helix‑hairpin‑helix 3 family protein/thioesterase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,3560AT 6.2% 164.6 / 2.2 60intergenic (+48/‑46)ybaV/fadMhelix‑hairpin‑helix 3 family protein/thioesterase III
Reads supporting (aligned to +/- strand):  ref base A (34/21);  new base T (2/2);  total (36/24)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

CCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGG  >  NC_000913/464222‑464498
                                                                                                                                      |                                                                                                                                              
ccGTTTAAAACGGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                                                                                                        <  1:1079826/143‑1 (MQ=255)
  gtgtAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACct                                                                                                                                      <  1:22193/140‑1 (MQ=255)
  gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAAGCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGGCATACAAGTTATGAAct                                                                                                                                      >  2:167485/1‑143 (MQ=255)
   tttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACctc                                                                                                                                     >  1:771803/1‑143 (MQ=255)
     cagaaCTTTGGAGTATCTAAAGCAGGTGCCGGGGATTGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATCGTGGCAATTTTTTGCCAGTCTGAAGAGGTTATACCAGTTATGACCTCTg                                                                                                                                   <  2:60970/140‑1 (MQ=255)
        aaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                >  1:923378/1‑143 (MQ=255)
        aaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                >  2:56960/1‑143 (MQ=255)
        aaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctc                                                                                                                                     <  1:236328/138‑1 (MQ=255)
        aaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctc                                                                                                                                     >  2:236328/1‑138 (MQ=255)
        aaCTGGCGAGGAGCAAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                <  1:311406/143‑1 (MQ=255)
             tgGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTtatc                                                                                                                           >  1:1040172/1‑142 (MQ=255)
                    tCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACGTATaacaacga                                                                                                                    >  2:903059/1‑141 (MQ=255)
                     cTATAGCATGTGCCTTGTATGTGGAATTCGTTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                   <  1:32853/143‑1 (MQ=255)
                     cTATAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAAAGTAATCTGGCGGTATTAACCCGGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                   <  1:297071/143‑1 (MQ=255)
                     cTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCACTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTAGaacaacaac                                                                                                                   >  2:1120174/1‑143 (MQ=255)
                               tGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCCGTTATGCCCTCTGTACTTATAACAACAACGTAAGGTTAt                                                                                                         <  1:267464/143‑1 (MQ=255)
                                gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGTCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTAtt                                                                                                        <  1:80940/143‑1 (MQ=255)
                                 ccGGGGATGGGCAATTCGCTGGTGGAACGTAAGCTGGCGGTATTAACCCTGCAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGACATACCAGTGATGACCTCTGTACTTATAGCACCAACGTCAGGTTATTg                                                                                                       >  2:591486/1‑143 (MQ=255)
                                  cGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCAAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTgc                                                                                                      >  2:236561/1‑143 (MQ=255)
                                     ggATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCGCTAATTATGACAACAAAGTTAGGTTAttaagct                                                                                                   >  2:94162/1‑138 (MQ=255)
                                        tgtgCAATTCGCAGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                                <  1:301911/140‑1 (MQ=255)
                                           gCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGcca                                                                                             >  1:179917/1‑141 (MQ=255)
                                            cAATTCGCTGGTGGAACGTAATCTGGCGGTCTTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCACACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCcaa                                                                                            >  2:391851/1‑143 (MQ=255)
                                            cAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTAGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTACGGATATTGAGCAATGaaaa                                                                                            >  2:331318/1‑143 (MQ=255)
                                                tCGCTGGGGGAACGTTATGTGGCTGTATTAACGCTGTAGTTGATTTGTATAGTGTCAATTTTTTGCCAGACTGAATAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAAcaca                                                                                        <  2:1068790/143‑1 (MQ=255)
                                                   cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGCGCTCTGTACTTATAACAACACAGTAAGGTTATAGAGATATGCAAAAACAAAt                                                                                     >  2:654283/1‑143 (MQ=255)
                                                   cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGACGTCATACCAGTGATGACCTCTGTACATAGAACAACAACGTAAGGTAATAGCGCCATGCAAACACaacc                                                                                     >  2:415125/1‑141 (MQ=255)
                                                   cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAAGTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAAt                                                                                     <  1:614613/143‑1 (MQ=255)
                                                     ggtggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCa                                                                                   >  2:623597/1‑143 (MQ=255)
                                                       tggAAGGAAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGGCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCaaa                                                                                 <  2:269517/143‑1 (MQ=255)
                                                           cggTAATGTGGCGGCATAAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTc                                                                             <  1:357201/141‑1 (MQ=255)
                                                             gTATTCTGGCGTTATTAACTCTGTAATTAATTTTCATAGTGGTAATTTTTTGCCAGACTGAAGAGGTACTACCTTACATAACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGt                                                                           <  1:1013516/143‑1 (MQ=255)
                                                                 tCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGTAAACACAAATCAAAGTTCGTGGat                                                                       >  1:79672/1‑143 (MQ=255)
                                                                     gCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCACTCACTAATCAAATTTCGTGGATatca                                                                   >  1:373817/1‑143 (MQ=255)
                                                                      cGGTATTAACGCTGTAATTAAGTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACCACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcat                                                                  <  1:718814/143‑1 (MQ=255)
                                                                              aCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGt                                                          >  1:652607/1‑143 (MQ=255)
                                                                                ccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCCACGttt                                                        >  2:119697/1‑143 (MQ=255)
                                                                                 cTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATCACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCCACGTTTa                                                       >  1:388300/1‑143 (MQ=255)
                                                                                 cTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGtttc                                                       >  2:217293/1‑142 (MQ=255)
                                                                                     aattaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAGCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTTGATATCATCTCGACGTTTACCAg                                                   >  1:1082559/1‑143 (MQ=255)
                                                                                                    tGGTAATTTTTTGCTAGATTTAAGAGGTCATACTAGTTATGTCTTGTGTACTTATAAGAGGAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGcc                                    <  1:781168/143‑1 (MQ=255)
                                                                                                      gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGATATTGCGCTATGCAAACACAAATCAAAGTTCCTGGATATCATCTCGACGTTTCCCAGCACGTCAACACCGCCCg                                  >  1:311067/1‑143 (MQ=255)
                                                                                                       cAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGc                                 <  1:388833/143‑1 (MQ=255)
                                                                                                       cAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACAAGCACGTCAACAACGCCCGc                                 <  1:94817/143‑1 (MQ=255)
                                                                                                         aatttttGCCTGACTGAAGAGGTCATACCAGTAATGACATCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGACAAGAACGACCGCTa                               >  2:25267/3‑143 (MQ=255)
                                                                                                          ttttttGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTAc                              >  1:342195/1‑143 (MQ=255)
                                                                                                             tttGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCtt                           >  2:216327/1‑143 (MQ=255)
                                                                                                               tGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGa                         <  1:403009/143‑1 (MQ=255)
                                                                                                                  cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAtt                      >  1:834890/1‑143 (MQ=255)
                                                                                                                  cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAtt                      >  1:495916/1‑143 (MQ=255)
                                                                                                                  cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATATCGCTGTTGAGCATCACGTCAACAACGCCCGCTACCTTGAAtt                      >  2:796020/1‑143 (MQ=255)
                                                                                                                   aGACCGAAGAGGTCAGACCAGTTAGGGCCTCTGTACTTATAACCACAACGTAAGGTGATTGCGCTACGAAAACACAAAACACAAATTGGAGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAttt                     <  2:239587/143‑1 (MQ=255)
                                                                                                                    gACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtc                    >  2:816604/1‑143 (MQ=255)
                                                                                                                    gACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAACCAACGCTCTATGTTGGGATCTCGACGTTTACCAGCACGTCAACAACGCCGGCTACCTTGAATTtc                    >  1:258527/1‑143 (MQ=255)
                                                                                                                       tacagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACATTTACCATCACGTCAACAACGCCCGCTACATTGAATTTCTCg                 >  1:39273/4‑143 (MQ=255)
                                                                                                                        gAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACACATCACAGATCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAACTTCTCga                >  2:772945/1‑143 (MQ=255)
                                                                                                                        gAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCga                <  2:652607/143‑1 (MQ=255)
                                                                                                                             ggTCATGCCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGaagaag           <  2:1004888/143‑1 (MQ=255)
                                                                                                                                     cAGTTATGAGCTCTGTACTTAGAACAACAACGTAAGGGTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACGACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTgg   <  2:771803/143‑1 (MQ=255)
                                                                                                                                     cAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGGAGCCCGCTgg   >  1:341413/1‑143 (MQ=255)
                                                                                                                                      aGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTggg  <  1:56960/143‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                              
CCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGG  >  NC_000913/464222‑464498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑