| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 543,094 | G→C | 10.6% | intergenic (+61/+167) | glxK → / ← allE | glycerate 2‑kinase 2/(S)‑ureidoglycine aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 543,094 | 0 | G | C | 10.6% | 169.4 / 13.0 | 63 | intergenic (+61/+167) | glxK/allE | glycerate 2‑kinase 2/(S)‑ureidoglycine aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (26/30); new base C (4/3); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 | |||||||||||
GCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAA > NC_000913/542953‑543227 | gCCAGCTGTGAAGCCAATCTCTTCAACTGCGCGCGTAATTTTGCCTGCGTTATTAAAATAGGTCAGGGAATTAAAAACTACCCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGc < 2:1119834/143‑1 (MQ=255) cGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt < 2:661827/143‑1 (MQ=255) tGAAACCAATCTCGTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAg < 1:756806/143‑1 (MQ=255) aaCAAATCTTGTCAACAGCGCGGGAAATATGGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TAc < 1:203163/143‑1 (MQ=255) atctacctctCCAACAGCGCGTAAAATATTTCCTTCTCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGAGGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TAcc < 2:604338/137‑1 (MQ=255) acgaaactctTGTACAGCGCGCGAAATTTTGTCCACGCCATTAAATGATGTGAGTATGTTTTAAACTGACCCTTTCCTTTAAAGCGGATGCGATGTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAG‑TAct < 2:737986/137‑2 (MQ=255) aaTCCCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAG‑TActgca < 1:543736/143‑5 (MQ=255) tctcTTCAACATCTCGCTAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATAtc < 1:296477/143‑1 (MQ=255) ttctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATAtcc < 2:1108149/142‑1 (MQ=255) ctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGCTTTCTAGCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATAtcctt > 1:518651/1‑143 (MQ=255) ttCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAATACTAACCCTTACCTTTAAAGCGGATGCGTTTTATATCGCATAAGAGTGCAGTATTCATGCAGGATGCGGCATGAA‑TACAatataatac > 1:589062/1‑138 (MQ=255) ttCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATAtccttg > 2:62512/1‑142 (MQ=255) tCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATAtccttcc < 1:216165/143‑1 (MQ=255) aaCAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTg > 1:982222/1‑143 (MQ=255) gcgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATc < 1:352209/143‑1 (MQ=255) gcgccAAATATTGCCTTCGCCATTAAAATAGGCCAGGGAATTAAAAACTAACGCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATc < 2:1061069/143‑1 (MQ=255) gctaaaTATTGCCTGCGCCATTAAAATAGGTAAGGGAAGTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATcgc < 1:897425/140‑1 (MQ=255) gAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCCTGAG‑TACCTTTTCCTTCTTGAAAATCgcgc > 1:234818/1‑143 (MQ=255) gAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCgcgc > 1:1046609/1‑143 (MQ=255) tatTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCacct > 1:421097/1‑140 (MQ=255) cgcCATTCAAATAGGTCAGGGAATTAAAAACTAAACCTTCCATTTTACGCGGATGAGATTTATATAGAATATGTGTGCAGTACTCATGCCGGATGCGGCATGTGTTAGCTTCT‑TTGCCTGAAAATCGCGCAAATTCTatatat < 2:553192/143‑1 (MQ=255) gcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATAtt > 1:1038108/1‑143 (MQ=255) ccATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTg < 2:741976/143‑1 (MQ=255) aGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt < 2:381418/143‑1 (MQ=255) ggTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGTGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGTTGTTTGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTAGATATTGCAGAGATCATg < 1:235047/143‑1 (MQ=255) cAGGGAATTAAAACCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATTCTCCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAg < 1:1018599/143‑1 (MQ=255) aGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTCTATATTGCAGAGATCATGTAgg > 1:133193/1‑143 (MQ=255) aGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGCGATCATGTAgg > 1:1022539/1‑143 (MQ=255) aGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAgg > 1:550533/1‑143 (MQ=255) gggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATACGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCATTCCTGAAACTCGCGAAAATTCTATATATTACAGAGATCATGTAGGc > 2:903325/1‑143 (MQ=255) gggAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGc > 1:1025823/1‑143 (MQ=255) aaaaaCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaa > 2:193944/1‑143 (MQ=255) aCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgc > 1:563403/1‑142 (MQ=255) cTAACCCTTACCTTTAAAGTGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TAGCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaa < 2:482868/143‑1 (MQ=255) cTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCCCGCAATTTCTATATATTGCAGAGATCCTGTAGGCCTGATaagcgaa > 1:940813/1‑143 (MQ=255) aaCCCTGACCTGTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcgaagc < 2:935216/143‑1 (MQ=255) tACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCGTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCa < 1:427419/143‑1 (MQ=255) aCCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAg < 2:715935/143‑1 (MQ=255) cTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGc > 1:902867/1‑143 (MQ=255) gCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGCTAAGCGAAGCGCATCCGGCACTGTTa > 2:1079166/1‑143 (MQ=255) caTTTATAACGCATAAGAGTGCAGTACTCAGGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaaaaa < 1:623783/142‑1 (MQ=255) aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAg > 1:1114191/1‑143 (MQ=255) tttATATCGCATAAGAGTGCAGTACTCATGCCGGATGGGGCATGAG‑TACCATATCCTTCATGAAAATCGCGCAAATTCGATATATTGCAGAGATCATGTATGCCTGATCAGAGAAGCGCCACAGGCAATGTTACAAAAAAAgg > 2:9843/1‑142 (MQ=38) tggatatCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCAGATCCATCCTGAAAATAGCGCAAATTCTATATATTGCAGAGATCATGTAGACCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGc < 2:279787/140‑1 (MQ=38) tataTCGAATAAGAGTGCAGTACTCAGGCCGGATGCGGCATGAG‑TACCATATCCTTCCGGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCa < 1:135927/143‑1 (MQ=255) tatCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACg < 1:789976/143‑1 (MQ=255) gCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGtat > 1:536577/1‑143 (MQ=255) aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATaa > 2:921209/1‑143 (MQ=255) aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATaa > 1:997188/1‑143 (MQ=255) agTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCACTGTTACCAAAAAAGCCACGGTATAAACCGt > 1:1030137/1‑143 (MQ=255) gcgCAGTACGCCTGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg < 1:62512/141‑1 (MQ=255) gTGGAGTAGTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg < 2:804448/143‑1 (MQ=255) cAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTTTATACCGTGGct < 1:401758/143‑2 (MQ=255) tgacgcATGCCGGAGCCGGCATTAG‑TACCCTATCCTTCCTGAACACCGCGCAAAGTCTATATACTCCAGCGATCAGGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaa < 2:94113/138‑1 (MQ=255) gTACTCATGCCGCATCCGGCATGCG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATCTCTTGCCGAGATCATGTAGGCCTGATAAGCGACGCGCATCAGCCCATGTTACAAAAAAAGCCACCGCATAAACCGTGGccac > 2:256153/1‑140 (MQ=37) gTACTCATGCCGCATCCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaa > 1:171643/1‑143 (MQ=255) tACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCaaaa > 1:1026152/1‑143 (MQ=255) aCTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAAt < 1:678808/143‑1 (MQ=255) cTCATGCCGCATCCGGCATGAG‑TACAATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCAACAGGCAATGTTACAAAAAAAGAAACGGGATAAACCGAGGCAAAATc > 2:289515/1‑143 (MQ=37) tCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCCTGTAGGCCTGCTACGCGACGCGCATCAGGCAATGTTACAAAAAAAGCCACGATATAAACCGTGACAAAATca > 1:301326/1‑142 (MQ=255) tCATGCCGCATCCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATCAGCGAAGCGCATCAGGCACTGTTACAAAAAACGCCACGGTATACACCGTGGCAAAAAcc > 1:742192/1‑143 (MQ=37) gtGCTGGATGCGGCATGTG‑TACCGTGTCCTTCATTACAATCGTGCAAATTCTATATGTTGCCGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa < 2:785227/142‑1 (MQ=37) aTGGCGGATGCGGCATGAG‑TACCCTATCCGTCCTGAGAATCGCGCTAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa < 2:156599/143‑1 (MQ=38) | GCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAG‑TACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAA > NC_000913/542953‑543227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |