| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 760,485 | G→A | 100% | G594S (GGC→AGC) | sucA → | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 760,485 | 0 | G | A | 96.2% | 110.7 / ‑5.2 | 44 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (20/21); minor base C (2/0); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTAC > NC_000913/760351‑760622 | ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCACAACATGTCACTC‑AAGGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCg > 1:519335/1‑143 (MQ=255) ggcgcTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCTGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg < 2:383447/139‑1 (MQ=255) gccggAGCTGGCGACACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg < 1:287031/140‑1 (MQ=255) cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑GAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGGGGTGCGGAAAACGTCGCTTACGc > 1:367588/1‑143 (MQ=255) aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGGTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGGAAACCTCGCTTACGcc > 1:942258/1‑143 (MQ=255) aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAACACCCCGCTTACGcc > 1:142376/1‑143 (MQ=255) tGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTg > 1:238664/1‑143 (MQ=255) aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTAGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgg > 1:38508/1‑142 (MQ=255) aCGCATCAGCACGGTGCCGGAAGCCGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg < 2:922109/143‑1 (MQ=255) aCGCATCAGCACGGTGCCGGAAGCATTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg < 1:172996/143‑1 (MQ=255) aCGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg < 2:463257/143‑1 (MQ=255) cGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGAGCTGGCTGACGCCACGCTGGCtgatga > 1:998025/1‑143 (MQ=255) gCATCAGCACGGTGCCGGAAGCAGTTGATATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 2:392686/143‑1 (MQ=255) gCATCAGCACGGTGCCGGAAGCAGTGGAAATGCAGGCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 1:27694/143‑1 (MQ=255) tagcAGCACGGTGCGGGAAGCAGTTGAAATGCACTCTCGCGCTGC‑CAAGATTTATGGCGGTCGCCGGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAg < 2:881013/140‑1 (MQ=255) cATGAGCACGGTGCCGTAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg < 2:472171/143‑1 (MQ=255) gTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCAATGGCTGCCGGTGAGAAACTGTTCGAATGCAGCGGTGCGGAAAACCTCGGTTACGCGACGCTGGATGCTGAGAGCGTTGCGGt > 2:311407/1‑143 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:624259/143‑1 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc > 1:265387/1‑143 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 1:961311/143‑1 (MQ=255) cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc > 1:166026/1‑143 (MQ=255) gCAGTTGAAATGCAGACTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGGTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGCTCCAGGCATTCCGGTTCGCCTGTc > 2:953789/1‑143 (MQ=255) ggTTGAAAGGCAGTCGCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 2:1071146/142‑1 (MQ=255) aGTGGAACTGCAGCCGCGCCTTGC‑CAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 2:1037791/143‑1 (MQ=255) ggtGAAATGCAGTCTCGCGTTGC‑CAAGATTTGTGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTTTCggg < 2:939150/141‑1 (MQ=255) gTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGTGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg > 2:1113832/1‑143 (MQ=255) cAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCACTCCGGCTCGCCTGTCGGGTGCGGACTc > 2:55324/1‑143 (MQ=255) aGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGAGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc > 1:732221/1‑143 (MQ=255) tctcGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTCATGACGCCCCTCCGGTTCCCCGCTCGGGGGAACCCTcccg > 2:1114458/1‑141 (MQ=255) tctcGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGCTGAAGGCATCCCGGCTCGCCTGTCGGGCGAACACTcccg > 2:120575/1‑141 (MQ=255) gcgTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGAGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgc > 1:423500/1‑143 (MQ=255) cgTTGC‑CAAGATTCATGGCGAGCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg < 1:345914/143‑1 (MQ=255) ttGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTCCGGCTTCCCTCCCGTATGGGCGGAATGAACAAGAAGGCATTCCGGTTCGCCCGTCGGGTGCAGACcgcggtcgcggt > 2:1103167/1‑143 (MQ=255) tttATTGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc < 2:829921/143‑1 (MQ=255) aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGCGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:784213/143‑1 (MQ=255) gCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGAGGGGTTGCGCCCTCACGCCGCGCATGGCGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg < 2:238664/143‑1 (MQ=255) ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 1:1029165/143‑1 (MQ=255) aaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACACGGTGATCCACAACCAGTCTAACGGt > 2:371622/1‑143 (MQ=255) ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCATTCCGGTTCGCCTGTCGGGTGAGGACTCCGGTCGCGGTGCCGTCTTCCACCGCGACGCGGTGGTCCACAACCAGTCTAACCGGTccgc > 2:101917/1‑141 (MQ=255) ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCAc > 2:24314/1‑143 (MQ=255) ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTACGGTCGCGGTACCTTCTTCCACCGCCACCCGGTGAACCACAGCCGATCTAAACGTCCCAc > 2:925086/1‑143 (MQ=255) tCGACGGGGGCGGGGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGGAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACTCGGTGATCCACAACCAGTCTAACGGTTCCACt < 2:589924/143‑1 (MQ=255) gACGGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCCCGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 1:311111/143‑1 (MQ=255) aCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTac < 1:483774/143‑1 (MQ=255) | TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTAC > NC_000913/760351‑760622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |