| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,169,384 | G→A | 7.4% | intergenic (+54/+28) | bhsA → / ← ldtC | DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA/L,D‑transpeptidase YcfS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,169,384 | 0 | G | A | 7.4% | 138.7 / 3.2 | 54 | intergenic (+54/+28) | bhsA/ldtC | DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA/L,D‑transpeptidase YcfS |
| Reads supporting (aligned to +/- strand): ref base G (24/26); new base A (2/2); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 | |||||||||||
AGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGA > NC_000913/1169243‑1169525 | aGCGGATTAGATGGGCGCAAAATCTTTCCGTATTACTTCTCGAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATGCGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt < 1:772042/143‑1 (MQ=255) cGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACTTGTCGGAATCGCCCCCATGGGGTAGCCCGTATCAAGAAACAAGCATTAACCCTCATTCATGCCTCCTACTGCTGATTTTTTCCCCGCGACCTGCCgtgt > 1:524302/1‑143 (MQ=255) gggaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgg < 2:814759/141‑1 (MQ=255) gagaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTTCATGGAACATCAGTAATTTATAAATAAGCATTAACCCGCATCAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCgg < 1:540071/143‑1 (MQ=255) gagaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCCGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgg < 1:865957/143‑1 (MQ=255) gcgcAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttt < 1:91349/143‑1 (MQ=255) cgcAAAATCTTTCCGTCTTATTTCTGTAACCGGTCCGAATTCCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttt < 2:813426/143‑1 (MQ=255) cgcAAAATCTTTCCGTATTACTTCCGTAACCGGTCCGAATACCCCCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCAGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttt < 2:815717/143‑1 (MQ=255) gcAAAATCTTGCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCACGGGGAtttttt < 2:509058/143‑1 (MQ=255) aaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGCTTTTTTtctc > 1:697412/1‑143 (MQ=255) tttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCggg < 1:698780/143‑1 (MQ=255) ccGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCtt < 1:873342/143‑1 (MQ=255) gTATTACTTCTGTAACCGGTCAGAATACCCTCCATGGAATAGCAGTAATTTATAAATAAGCATTAACACTCATTAATGCCTGCTACTGCTGATAATTCCCGCGCGTCAGGCCGTGTCGCCCGGGTTTTATTCGCCGGGcatca > 2:798433/1‑139 (MQ=255) tATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTAc > 1:735338/1‑143 (MQ=255) ttACTGCAGTAACCGGGCCGAATACCCTACATGGAACAGAAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCTCGACATGCCGTGTCGCGGCGATTTTTTTATCCGGGCTTTACAg < 1:653640/143‑1 (MQ=255) ttCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAGATAAGCATTAACGCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCTGGGCTTTattacgtt > 1:1076093/1‑136 (MQ=255) tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGAATTAACCCTCATTAATGCCTGCTCCTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTGTTTATCCGGGCTTTACAGCGTTTGTgg > 2:375860/1‑143 (MQ=255) tCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACt > 2:1005355/1‑143 (MQ=255) ccGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGGGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGGTTGTGG‑‑GCTCACtt > 1:1086634/1‑143 (MQ=255) ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGAcgg > 1:538810/1‑143 (MQ=255) ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGAcgg > 1:597633/1‑143 (MQ=255) cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGAcggc > 2:653127/1‑143 (MQ=255) cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGa < 1:1056212/143‑1 (MQ=255) cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGa < 1:233631/143‑1 (MQ=255) ctGGAACAGCAGTAATTTATAAATAAGCAGTAGCCCTCATTCATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGAc < 2:394134/142‑1 (MQ=255) gcagTAATTTCTAGTTACGCATTAGCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCTCTTGATGACGGCGGACATCCACCg < 2:526881/143‑1 (MQ=255) gTAATTTATAAATAGGCATTACCCCTCTTTAATGCCTGCTACTGCTGTTTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCa < 2:289989/143‑1 (MQ=255) tAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACGGCGTTTGTGG‑‑GCTCTCTTGCTGAGGGCCGGCGTCCATCggtct > 2:695023/1‑140 (MQ=255) aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGTTGACGGCGGACATCCACCGGCATCCCGGAAc > 1:881609/1‑143 (MQ=255) aaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAATTTTTTATCCGGGCTTTACAGCGTTTGAGGAAGAACACTT‑CTG‑CGGCGGACAAGCCCCGGCATCCCGGAACggcc > 1:818673/1‑141 (MQ=255) cactaccccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGGCGCGGGGAGTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatc < 1:447240/137‑1 (MQ=255) cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGGCGGCGGGCCTCCACCGGCATCCCGGAACGGCcatc > 1:716298/1‑143 (MQ=255) ttAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTGTTTATCCTGGCTTTACAGCGTTTGTGG‑‑GGTGACGTGATGACGGCGGCCATCGACCGGCACCCAGGAACGGAcgacca > 2:392271/1‑138 (MQ=255) agcccTCATTAATGGCTGCTCCTGCTGTTTTTTTCCCCGCGACATACCGTGTCGCGGGTATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatc < 2:101382/141‑1 (MQ=255) ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcaca < 1:1057772/143‑1 (MQ=255) gttAATGCCTGCTACTGCTGATTATTTCCCGGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTGCAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt < 2:1130333/142‑1 (MQ=255) aaTGCCTGCTACTGCTGATGTTTGCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa < 1:538195/143‑1 (MQ=255) aTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCAt < 1:707249/143‑1 (MQ=255) cTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACGTGTTGCGTCCCt > 2:157120/1‑143 (MQ=255) gCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGTCGGACATCCACCGGCATCCCGGAACGGAAACACATCACATGTTGCATTACTTc > 1:641678/1‑143 (MQ=255) gCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTc > 1:631110/1‑143 (MQ=255) cTACTGCTGTGTTTTTTCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTGACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCa < 2:717576/143‑1 (MQ=255) ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTATTATCCCGGATTTGGGTCGATTGTGG‑‑GCTCATACGCTGACGGCGGACATCCACAGGCATCCCGGTCCCGCCATCCATCACATGTTGCATCACTTCAGCGTCa > 1:523781/1‑143 (MQ=255) gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCCCCGGCATCCCGGCACGGACATCCATCACCTGTTGCCTCACTTCAGCGTCAGt > 2:236052/1‑143 (MQ=255) aTTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCGCTTGCTGACGGCGGACATCCACCGGCATCGCGGAACGGACATCCATCACATGTTGCATCACTGCACCGTccgct > 2:319704/1‑139 (MQ=255) aTTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGGGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCGTCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGtt > 1:363485/1‑143 (MQ=255) ttttttCCCCGGGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGttt < 2:1075025/143‑1 (MQ=255) tttgttCCCCGCGACACGCCGGGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGttt < 2:962457/143‑1 (MQ=255) ccGCGACACGGCATGTCGCGGGGATTTTTTTCTCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGACATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCCGTTTGTGCTGCa > 1:167212/1‑143 (MQ=255) ccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑ACTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGAGTAAATTAGTGCTGCa > 1:821824/1‑143 (MQ=255) cgcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCAt < 1:26938/143‑1 (MQ=255) cgcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCAt > 1:117591/1‑143 (MQ=255) cgcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACAGCCATCACAGCTTGCATCGCTTCAGCGTCAGTTTGTCCTCCac > 2:833692/1‑142 (MQ=255) tGCCGTGTCGCGGGGATTTTTTTAGCCGGGCGTTAGAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa < 1:327509/143‑1 (MQ=255) tGCCGGGTCGGGGGGAGTTTTTTATCCGGGTTTTACAGCGGTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa < 2:315031/143‑1 (MQ=255) cGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGa < 1:1005355/143‑1 (MQ=255) | AGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGG‑‑GCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGA > NC_000913/1169243‑1169525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |