Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,887 C→T 9.3% intergenic (+39/‑141) ilvH → / → cra acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit/DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8870CT 9.3% 157.6 / 5.3 63intergenic (+39/‑141)ilvH/craacetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit/DNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base C (26/31);  new base T (3/3);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGT  >  NC_000913/87748‑88029
                                                                                                                                           |                                                                                                                                                
ttGAGGCGTGTTTGGCATCGATTCGCGAGGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGtt                                                                                                                                               <  2:434508/143‑1 (MQ=255)
   aTGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAGTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTggg                                                                                                                                            >  2:909176/1‑143 (MQ=255)
   aTGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTggg                                                                                                                                            <  1:954188/143‑1 (MQ=255)
   aTGCATTGTTAGCATCGACTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCGCTGGTGTGGTCGGACTTTCGCGCGGCGATACAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTggg                                                                                                                                            <  2:560379/143‑1 (MQ=255)
    tGCCTTTTTAGCATCGATGCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGc                                                                                                                                           <  1:475784/143‑1 (MQ=255)
           ttAGCCTCGATTCGCGATATGGCGAAAATTGTGGAGGTGGCTCGCTCTTGTGTGGTCGGACTTTCGCGCGACGATAAAATAATGCGTTGAGAATTATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑ttttttt                                                                                                                                   <  1:486482/143‑1 (MQ=255)
           tgggggtCGAGTCGGGGTGTGGCGAATATTGGGGAGGTTGCGCGCTCTGGTGGGGTCGGACTTTCGCGGGGTGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑ttttttt                                                                                                                                   <  2:201231/137‑1 (MQ=255)
                aTCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATCATGCTTTGAGAATGATCTGCATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTGTTTTGCGaa                                                                                                                              >  1:704979/1‑143 (MQ=255)
                 tCGATTCGCGATGTGGCGAAAGTTGTGGAGGGTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGaaa                                                                                                                             <  2:1071933/143‑1 (MQ=255)
                 tCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGTGCAATTTACAGGCGGACGTGTGATGTGGGGC‑TTTTTTTGCGcaa                                                                                                                             >  1:153064/1‑143 (MQ=255)
                  cGATTCGTGATGTGGCGAAAATTGTGGAGGTTGCTCGCTATGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAAt                                                                                                                            <  1:810066/143‑1 (MQ=255)
                      tCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGGGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGt                                                                                                                        >  2:912941/1‑143 (MQ=255)
                       cggtaTGTTGCTACAATTGTGGAGGTTGCTCGCTCTTGTGTCGTTGGACTTTCCCGCATCGATAATATAATGCGTTGAGAATGATCTCAATTCGCAATTTACATCCCATCATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTg                                                                                                                       <  2:27829/139‑1 (MQ=255)
                       cgcgATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGAGTTTGGAGCACCAATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTg                                                                                                                       <  1:519471/143‑1 (MQ=255)
                        tggATGTGGCGAAAATTGTGGAGGTTGCTCGCTCGGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTgg                                                                                                                      <  2:43507/141‑1 (MQ=255)
                           attgggCTAAAATTGTGGATGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCTGCGATAAAATGATGTGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGaa                                                                                                                   <  2:917882/139‑1 (MQ=255)
                                gCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCACTTTACACCCCAACATCTCACCTTGGGC‑TTTTTTTGCGAAACCAGTGGGGCCCTgg                                                                                                              >  2:203780/1‑143 (MQ=255)
                                  gAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCCGTGGGAACATGGaa                                                                                                            >  2:276577/1‑143 (MQ=255)
                                       tgggtgAGGTTGCGCGCTGTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑aaaa                                                                                                      <  2:768006/138‑1 (MQ=255)
                                       gtgtgGAGGTTGCTCGCTGTGGTGTGGTCGGACGGTCGCGCGGCGATAAAATAAGGCGGTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑aaaa                                                                                                      <  1:226538/142‑1 (MQ=255)
                                               gTTGCCAGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGc                                                                                              <  1:379944/143‑1 (MQ=255)
                                                gtgccggctcGGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAGTTTACAGCGTAGCATGTCACGTTGGGG‑TTTTTTTGCGTAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGcc                                                                                             <  1:1050680/137‑1 (MQ=255)
                                                 ttctcgttcTGGTGGGGTCGGACTTTGGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACCGGCCAACATGTCACGTTGGGC‑TATTTTTGCTAAAGCAGTAGGAACCTGGAAT‑AAAAGCAGTTGCCg                                                                                            <  2:845311/141‑1 (MQ=255)
                                                  gcgcgctcGGGTGTGCTCGGATTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAAGTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGCGGGAACCTGGAAT‑AAAAGCAGTTGCCGc                                                                                           <  2:140800/140‑1 (MQ=255)
                                                   ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCa                                                                                          <  1:897298/143‑1 (MQ=255)
                                                        tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGTAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                                     >  1:226212/1‑143 (MQ=255)
                                                        tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                                     >  1:223925/1‑143 (MQ=255)
                                                        tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGCC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑ACAAGCAGTTGCCGCAGTTaa                                                                                     >  2:698267/1‑143 (MQ=255)
                                                        tctGGTGTGGTCGGACTTTCGCGCGGCGAGATGATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTaa                                                                                     >  2:990405/1‑143 (MQ=255)
                                                         ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAt                                                                                    <  2:876460/143‑1 (MQ=255)
                                                           ggTGGGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAttt                                                                                  <  1:221324/143‑1 (MQ=255)
                                                               tgGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                              >  1:314680/1‑143 (MQ=255)
                                                                 ggcGGACTTTCGCGCGGCGATAAAATAAAGCGATGAGAATGAGCACAATGCGCAAGTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATGTTCTgcg                                                                            >  2:455965/3‑143 (MQ=255)
                                                                  tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCAAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCACTGGGAACCTGGAAT‑AAAAGCAATTGCTGCAGTTAATTTTCTgcgc                                                                           >  2:295819/1‑143 (MQ=255)
                                                                   cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCCACATGTCCCGTTGGGC‑TTTGTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCGGCAGTTAATTTTCTGCGCt                                                                          >  2:130585/1‑143 (MQ=255)
                                                                       cTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                                                                      <  1:745433/143‑1 (MQ=255)
                                                                         ttCGCGCGGCGATAAAATAATGCGTTGAGAATGTTATCAATGCGCGATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCAGGAGTTAATTTTCTACGCTAAGATg                                                                    >  2:1078576/1‑143 (MQ=255)
                                                                          tCGCGCGGCGATAAAATAATACGTTGTGAGTGATCTCAATGCGCAATTGACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                                                                   <  1:417399/143‑1 (MQ=255)
                                                                              gcggccATAAAATAATGCGTTGAGAATGATCCCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAAt                                                               <  1:953094/143‑1 (MQ=255)
                                                                                ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑‑TTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGCTGTTAATGaa                                                            >  1:649770/1‑143 (MQ=255)
                                                                                agcgATAAAATAATGCGTTCAGAAGGATCTCAATGCGCAATTGACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGa                                                             <  1:563934/142‑1 (MQ=255)
                                                                                   gttAAAATAATGCGTTGGGGATGATCTCAAGGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt                                                          <  2:158773/141‑1 (MQ=255)
                                                                                   gATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGTGGCCATTAATTTTCTGCGCTTAGATGTTAATGAAtt                                                          <  2:1110356/143‑1 (MQ=255)
                                                                                     tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTa                                                        >  2:214844/1‑143 (MQ=255)
                                                                                        aatgatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAcc                                                     <  1:409094/143‑1 (MQ=255)
                                                                                                 ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGt                                            >  1:1032236/1‑143 (MQ=255)
                                                                                                 ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCAATACCAGt                                            >  1:143236/1‑143 (MQ=255)
                                                                                                 ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCGTAGATGTTGATGAATTTAACCTATACCAGt                                            >  1:876393/1‑143 (MQ=255)
                                                                                                  tGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAC‑AAAACCAGTTGCCGCCATTAATTTTCAGCGCTTCGATATCAATTAATGTAACCCTTACCAATa                                           >  2:849392/1‑143 (MQ=255)
                                                                                                   gagaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTAc                                          >  2:1112004/1‑143 (MQ=255)
                                                                                                          aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCACTACAATGGcc                                   >  2:950370/1‑142 (MQ=255)
                                                                                                             tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTAt                                 <  2:212072/143‑1 (MQ=255)
                                                                                                               aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCCCTTAGATGTTAATGAATTTAACCCATACCAGTACAAGTGCAATGGt                              >  2:51057/1‑143 (MQ=255)
                                                                                                                 tGCGCAATTTACAGCCGAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTAACCGCAGGTATTGTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttt                            <  2:187628/143‑1 (MQ=255)
                                                                                                                     cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTa                         >  1:99743/1‑143 (MQ=255)
                                                                                                                        tttACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGGCGGAACGTTGAGA‑ATGATGAGTTGCCGCAGTGAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAtt                     <  1:258077/143‑1 (MQ=255)
                                                                                                                        tggagaGCCGAAGATGTAACGTTGGGC‑TTGTTCTGAGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTGAATTTGCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAtt                     <  2:769774/138‑1 (MQ=255)
                                                                                                                           aCAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTa                  <  1:264877/143‑1 (MQ=255)
                                                                                                                             aGCCCAACGTGACATGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAAGTTTCTGCGCTGAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                >  1:685111/1‑143 (MQ=255)
                                                                                                                             aGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGTAT‑AAAAGCAGATACCGCAGTTAATTTTATGAGATTAGATGATAATGAATTTAACCCATACTAGTACAATGGCTATGGTATTTACATTTTACg                >  1:661123/1‑143 (MQ=255)
                                                                                                                               cccAACGTGACATGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCa              >  2:881198/1‑143 (MQ=255)
                                                                                                                                   aCATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg          >  1:541758/1‑143 (MQ=255)
                                                                                                                                         cACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTGAATGAATTGAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAAtt    <  1:986960/143‑1 (MQ=255)
                                                                                                                                           cGTTGGGC‑TTTTTGTGCGAAATCAGTGGGAACGTGGAAT‑AAAAGCAGTTGCCGCAGTCAATTTTCTGCGCGTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATtgt  <  1:705413/143‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
TTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGC‑TTTTTTTGCGAAATCAGTGGGAACCTGGAAT‑AAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGT  >  NC_000913/87748‑88029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑