| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,684,019 | C→T | 10.5% | R380W (CGG→TGG) | rstB → | sensory histidine kinase RstB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,684,019 | 0 | C | T | 10.5% | 120.7 / 5.9 | 51 | R380W (CGG→TGG) | rstB | sensory histidine kinase RstB |
| Reads supporting (aligned to +/- strand): ref base C (21/23); new base T (2/3); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01 | |||||||||||
CCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATG > NC_000913/1683886‑1684152 | ccATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCCTTGAACCTTTTGT‑ACGCCTCGATCGCCGACGGGATCgtt > 2:250126/1‑141 (MQ=255) gaTTCAACGGTTGAACCCAGCCTGCTACTGTCGGGGAATAGAGCGACATGAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCGGCTGGGATCgagg < 1:1101814/142‑4 (MQ=255) cATTTAGCGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATCGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCGGCTGGGATCgagg < 2:506318/143‑4 (MQ=255) tCATCGGTTGAATCCAGCCTGCTACTGTCGGGGAATAGAGCGACAGTAATAGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCGGCTGGGATCgaggcga < 1:531647/143‑7 (MQ=255) gAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTGGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTg < 2:225730/143‑1 (MQ=255) aaaCCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGc > 1:549904/1‑143 (MQ=255) aaCCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCggcggccacg > 2:366898/1‑139 (MQ=255) ccAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGTGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCggg < 2:280311/143‑1 (MQ=255) gCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctg > 1:559566/1‑143 (MQ=255) tGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGG‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctgggg < 1:432253/143‑1 (MQ=255) cgccaCTGTCGGGGAATAGAGCGACATTAATCGTCGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACACATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctgggg < 1:544181/139‑1 (MQ=255) tACTGTCGGGGAATAGAGCGACATTAATTGGTGAGGATGATGGCCCAGGGATTGCCCCAGAGAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctggggctg < 1:96044/143‑1 (MQ=255) aCTGTCGGGGAATAGAGCGAAATAAATTTGTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGggctggggctgg < 2:442737/143‑1 (MQ=255) gTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCaa < 1:111765/143‑1 (MQ=255) gggAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGt < 1:1049370/143‑1 (MQ=255) gaaaTAGGGCGGCGTTAATTGTTGAGGTTGATGCCCCAGGCATTGCCCCAGAAAACCGCGAACATATCTTCGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc < 1:227078/141‑1 (MQ=255) gagtAGAGCGACAGTAACTGTGGGGGATGATGGCCCAGGGATTGCCCCAGAAATCCGCGAACATATCTTTGAACCTTTTGT‑TCGCCGCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTcc < 1:586274/140‑1 (MQ=255) gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCActct < 1:1109842/143‑1 (MQ=255) gCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAAGTGTCCACTCtat > 1:539890/1‑143 (MQ=255) atttctcaGGACGATTGCCCAGGGAGTGCCCCAGAAAACCGCTAACCTATCTTTGACCCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGTTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACCGGc < 2:987910/136‑1 (MQ=255) gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATggg < 1:888409/143‑1 (MQ=255) gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCCCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTGTAGCACTGGCCGTggg > 1:854184/1‑143 (MQ=255) gatgGTTCAGGGATTGCCCCAGAAAACCGCGAGCAGATCTTTGAACCTGTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTAc < 1:531420/143‑1 (MQ=255) gatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTAc > 1:840889/1‑143 (MQ=255) atgGCCCAGGGCTGGCGCCAGAAAACCGCGAACATATCTTTGAACCTTTGTT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACg < 1:591500/143‑1 (MQ=255) ggCCCACGGCTTGCCCGCGAACTCGTCGAACTTAGGTTTGCACCTTGTGTCTCG‑CTCGATCCCGGCCGGGATATCTCAACCGGAGGCTGCGGGCTGTGGTTGGTAATTGTCCATTCCATAGCACTGGCAATGGGCGGTACGGt < 2:1034010/143‑1 (MQ=255) gCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCCACCGGCGGCTGCGGGCTGGGGCTGGCAAATCTCCACGCTATAGCACTCGCAATGGGCGGTACCGtc > 1:248053/1‑142 (MQ=255) gCCCCCGGAAACCGCGAAGAGATCTGTGAACCTTTTGT‑GCGCCTCGATCCCAGCGGGGATCGTTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGGAATGGGCGGTACGGTTAATTGTGacac < 2:563149/143‑1 (MQ=255) cccAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCa > 1:116456/1‑143 (MQ=255) ccAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAg < 1:113225/143‑1 (MQ=255) aaaCCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGGTAGTTGTGACACCAGCGGAc > 1:563285/1‑143 (MQ=255) aCCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGGTGATTGTGGAGCCAGCGAAccg > 1:698565/1‑141 (MQ=255) cgcgAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTcgg > 2:107325/1‑143 (MQ=255) cgAACATATCTTTGAACCTTGTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGggtg < 2:487795/143‑1 (MQ=255) accATATCTTTGAACCTTTTGT‑CCGCCTCGACCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCAGCACGGTTAATTGTGACACCAGCGAACTGggtggt < 1:267298/141‑1 (MQ=255) cATATCTTTGATCCTTTTGT‑CCGCCTCGATCCCAGCCGGGATCGCTCAGCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGc < 1:1101349/143‑1 (MQ=255) cATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGc > 2:1005445/1‑143 (MQ=255) tatCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAGTTGTCCACTCTATAGCACTGGCAATGGGCGGTAAGGATAATTGGGAGACCAGCGAACTGGGTGGGGccc > 2:66665/1‑143 (MQ=255) tatCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATCGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGccc > 1:109119/1‑143 (MQ=255) tatCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGccc > 1:972497/1‑143 (MQ=255) tttGAACCTTTTGT‑TCGCCTCGAGCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCGGGCAATTGTCCTCTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCtt < 2:776543/143‑1 (MQ=255) gAATCTTTAGT‑TCGCGACGATCCCAGCAGGGATCGGTCAACCGGCGGCGGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtctc < 1:275802/143‑1 (MQ=255) ccTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCCATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGGTAATTGTGACACCCGCGAACTGGGTGGCGCCCGCTTCTCGtt > 1:393113/1‑143 (MQ=255) ccTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt < 1:1005445/143‑1 (MQ=255) ccTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt > 2:361693/1‑143 (MQ=255) ttttGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTa > 2:586864/1‑143 (MQ=255) ttttGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTa > 1:843491/1‑143 (MQ=255) ttttGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTa < 2:840889/143‑1 (MQ=255) gagcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGGGACGAGCACACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGt > 1:515513/4‑143 (MQ=255) gcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGGTTAGCTGGCCGTTa > 2:460915/2‑143 (MQ=255) gATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGGTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGGTTAGCGGGCCGtgctc > 1:94876/1‑139 (MQ=255) | CCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGT‑TCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATG > NC_000913/1683886‑1684152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |