Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 6.3% intergenic (+29/+19) yeaD → / ← yeaE putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 6.3% 182.6 / 4.4 64intergenic (+29/+19)yeaD/yeaEputative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE
Reads supporting (aligned to +/- strand):  ref base A (26/33);  new base T (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

GCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGT  >  NC_000913/1864625‑1864904
                                                                                                                                          |                                                                                                                                             
gCTACAAAACATTTGTTTGTGTAGAAACGGCTCACGCTTCAGAAACGCAAACAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCa                                                                                                                                           <  2:312852/143‑1 (MQ=255)
 ccaCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACCTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCAt                                                                                                                                          <  1:619227/141‑1 (MQ=255)
  tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATc                                                                                                                                         >  2:157372/1‑143 (MQ=255)
  tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATc                                                                                                                                         >  2:132550/1‑143 (MQ=255)
    cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                       <  2:653303/143‑1 (MQ=255)
     aaaaTATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                      <  2:1017074/143‑1 (MQ=255)
     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCGCATCTGGCGCAATCCATTCGGGTTGCGAAACGTCAAGTTACGCTAATGTTGTGTGCCGGGTGCACTGCATccag                                                                                                                                      >  1:339198/1‑141 (MQ=255)
     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                      <  2:577920/143‑1 (MQ=255)
      aaaCATTTGGTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATCCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                     <  2:885314/143‑1 (MQ=255)
         gaTTTGTGTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                  <  1:462127/142‑1 (MQ=255)
         cATTGGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                  <  1:264891/143‑1 (MQ=255)
              gtttgtGTAGAAACGGCTTACGCTTAAGAAACGCAAAAAGTGACCAAAGAGAAACCTGAAAATCTGACGCAATTCATTCGCGTTTCGAAACGGTAATTAACGTTAACGTTGTGTTCCTGGTGAAATGCAACCGGcacactata                                                                                                                             >  2:999825/1‑139 (MQ=255)
               tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAt                                                                                                                            >  1:970514/1‑143 (MQ=255)
                   tgtAGACACGGCTGACGCGCCAGAAACGCAAAAAGTGACCACAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                        <  2:1036039/143‑1 (MQ=255)
                   tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                        >  1:558950/1‑143 (MQ=255)
                    gtAGCAACGGCTTACGTTTTAGAAACGCAAAAATTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCacac                                                                                                                       <  2:29701/143‑1 (MQ=255)
                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCccac                                                                                                                       >  1:112039/1‑143 (MQ=255)
                        aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                   >  1:283655/1‑143 (MQ=255)
                          aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAGCATCACACCatata                                                                                                                 >  1:142865/1‑142 (MQ=255)
                              cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                             >  2:306799/1‑143 (MQ=255)
                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                            <  1:594993/143‑1 (MQ=255)
                                 aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCATACCATATCCAGCGCa                                                                                                          <  2:937341/143‑1 (MQ=255)
                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTTTGCCGGGTGCAATGCATCAGGCACACAGCATCACACCATATCCAGCGCAggt                                                                                                       >  1:118142/1‑141 (MQ=255)
                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                    <  2:198045/143‑1 (MQ=255)
                                        aGAAACGCAAAAAGTGACCGAAGAGAAACCTGCACATCGGGCGCAATCCAGTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                   <  2:538438/143‑1 (MQ=255)
                                        aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                   <  1:786778/143‑1 (MQ=255)
                                        aGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGCGCAGTTTTTc                                                                                                   <  1:1023714/143‑1 (MQ=255)
                                        aGAAACGCAAAAAGGGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                   <  2:193170/143‑1 (MQ=255)
                                              gCAAAAAGTGGCCAAGGAGAAACCCGCGCATGTGGCGCAATCCATTCGCGTTGCGAAACGTTAATCTACGGTAATGTTGTGTGCCGGGTGGAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                             <  2:520252/143‑1 (MQ=255)
                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                           >  1:226769/1‑143 (MQ=255)
                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                           >  1:1134717/1‑143 (MQ=255)
                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAAGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGAATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                           >  1:553436/1‑143 (MQ=255)
                                                  aaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                         <  2:318571/143‑1 (MQ=255)
                                                  aaaGTGACCAAAGAGAAACCTGCACAGCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                         <  1:506679/143‑1 (MQ=255)
                                                    tgTGACCAAAGGGAATCCCGCGCATCTGGCGCACTCCCGTCGCGTTGCGAAACGTTATTTTACGCTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                       <  2:900529/142‑1 (MQ=255)
                                                         caaaaGAGAAACCTGCACATCGGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                  <  1:526222/141‑1 (MQ=255)
                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCAAGCGCAGTTGTGCCTTTTGGTGCCGGAtgtg                                                                                 >  1:665514/1‑143 (MQ=255)
                                                                 tacccTTCACATCTGGCGCAATCCATTCGCGGTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCCGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                          <  1:714774/140‑1 (MQ=255)
                                                                 aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATCTGCCTTATc                                                                          >  1:13884/1‑143 (MQ=255)
                                                                    ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCGCACAACATCACACCATATCCGGCGCAGTTTTTCCTTGTGGTGCAGGAGATGCCTTATCCAg                                                                       >  2:728059/1‑143 (MQ=255)
                                                                        cacaCCTGTCGCAATCCATTCGCGTTGCGAAACGTGAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                   <  2:1115858/143‑1 (MQ=255)
                                                                                gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTACTTc                                                           >  1:376047/1‑143 (MQ=255)
                                                                                    aacccgttCGCGTTGCGAATCGTTAATGTCCGTTAATGGTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                       <  2:811457/137‑1 (MQ=255)
                                                                                      tCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                     <  1:306799/143‑1 (MQ=255)
                                                                                        ccttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGCGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                   <  2:139380/141‑1 (MQ=255)
                                                                                              cgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                             <  1:948379/143‑1 (MQ=255)
                                                                                                     aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                    <  2:143255/129‑1 (MQ=255)
                                                                                                     aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                    >  1:143255/1‑129 (MQ=255)
                                                                                                     aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTc                                      >  2:23395/1‑143 (MQ=255)
                                                                                                       aCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCaa                                    <  1:1033942/143‑1 (MQ=255)
                                                                                                       aCGTTAATTTACGTCAATGTTGTGCGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAATTTCGACCTCaa                                    <  2:788407/143‑1 (MQ=255)
                                                                                                        cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                   <  1:132550/143‑1 (MQ=255)
                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCCCAGCCGCAt                        >  1:198782/1‑143 (MQ=255)
                                                                                                                   gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATACAGAGAAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACATCAAGCACAgcagcag                        >  2:398677/1‑142 (MQ=255)
                                                                                                                         gttgtGTGCCGGGTGCAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTACTTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                  <  2:142865/143‑1 (MQ=255)
                                                                                                                            gtgtgCCGGGTGCAATGCATCCGGCACTCTTATTCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa               <  2:848397/143‑1 (MQ=255)
                                                                                                                               tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCAGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCGCCACAGCCGCATTTTGTTTGAccg            >  1:195673/1‑141 (MQ=255)
                                                                                                                               tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGCCac            >  2:85219/1‑142 (MQ=255)
                                                                                                                                 ccGGGTGCAATGCATCCGGCACACACCATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg          <  2:461241/143‑1 (MQ=255)
                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGGTATACCCTATCCAGCATCGCTAATTCCGCTGACGAGAGTGCGACCGAAAGCACAGCCGCGTTTTGTGGGAcgagg          >  2:355491/1‑139 (MQ=255)
                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGGCTTATCCAGCATAGCTAATTCGGCTGAAGAAAGATCGACTTCCAGCACAGCTGCATTTTGTGGGGGATgg          >  2:1044437/1‑143 (MQ=255)
                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAACCACAGCCGAATTTTGTTGGACATgg          >  1:492678/1‑143 (MQ=255)
                                                                                                                                 ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATCTGCCTTATCCAGCATAGCTAATTCCACTGAAGAAAGTTCTACCTCAAACACAGCCGCATTTTGTTGGACATgg          >  1:893331/1‑143 (MQ=255)
                                                                                                                                 ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGAGCCGGATATACCTAAAAAAAAATAGATAATACCGCTAAAGAAAGTTAGACCTCAAGCACAGCCGCATTTTGTTGGACATgg          >  1:296516/1‑143 (MQ=255)
                                                                                                                                         aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCAGAGCCGCATTTTGTTGGACATGGGCAGTCGt  >  2:493070/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                             
GCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGT  >  NC_000913/1864625‑1864904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑