Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,883,027 C→G 6.5% intergenic (+30/‑161) dmlA → / → yeaV D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,883,0270CG 6.5% 179.9 / 5.6 62intergenic (+30/‑161)dmlA/yeaVD‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)/putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base C (34/24);  new base G (2/2);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

CTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCG  >  NC_000913/1882887‑1883162
                                                                                                                                            |                                                                                                                                       
cTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTAACTg                                                                                                                                       >  1:639585/1‑143 (MQ=255)
        aaTTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGCTTACCAACAGAATTTTGCGGTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGtt                                                                                                                               >  1:823748/1‑143 (MQ=255)
        aaTTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGtt                                                                                                                               >  1:611642/1‑143 (MQ=255)
         aTTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTATGCGTTACAGCCCAACCAGTTTATTGGAACCGCGGCACTGAcgcgtgta                                                                                                                              >  2:285751/1‑139 (MQ=255)
           tGAAGTAGTGATTGTTCACGGGGCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCGGttcaa                                                                                                                            <  1:984037/143‑4 (MQ=255)
             aagaagTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGATGCCGACGCGATTTGCAAAATCATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGtatttct                                                                                                                          >  2:880663/1‑141 (MQ=255)
              agaagTGATTGCTCACGGGCCGACAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTa                                                                                                                         <  1:861312/143‑1 (MQ=255)
               gaagTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTAt                                                                                                                        >  1:500715/1‑143 (MQ=255)
                 agTTATTGCTCACTGGTCGAAAACACCTGATATGAAAGGCAATGCCACTACCCCACAGTTTGCCGACGCGATTTGCAAAATTATTTTGCTTTAAGGTCAAACCATTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTc                                                                                                                      <  2:997521/143‑1 (MQ=255)
                    gATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGGATCGTTATTCGtt                                                                                                                   >  2:104739/1‑143 (MQ=255)
                                gCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTaaa                                                                                                       >  2:848117/1‑143 (MQ=255)
                                  cGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATa                                                                                                     >  1:991698/1‑143 (MQ=255)
                                    aaaaCACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAACTTATCTCGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACGGACGCGGATTATTTAGTCGTTCTTTGCAGTAAATAAc                                                                                                   >  2:65758/1‑143 (MQ=255)
                                         acCTGGTATGAAAGGCAATGCCACCACGCCACATGTTGCCTGCGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCg                                                                                              <  2:121861/143‑1 (MQ=255)
                                         acCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCg                                                                                              >  1:200511/1‑143 (MQ=255)
                                              atatGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCGAAAGCCTGGTGCCTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCAtt                                                                                         >  2:805872/1‑143 (MQ=255)
                                                 tGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCAGTTCa                                                                                      >  1:266543/1‑143 (MQ=255)
                                                   aaaGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGCTTTTTTAGTCGTTCTTTGCAGTAAATAACCTGCGTCCTTTCAcc                                                                                    >  1:359646/1‑143 (MQ=255)
                                                        cAATGCCACCACGCCCCAGTATGCCCACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTa                                                                               <  1:1062373/143‑1 (MQ=255)
                                                         aaTGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTAt                                                                              <  1:236299/143‑1 (MQ=255)
                                                           tGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACAAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGCCCTTTCACCTTTTATTg                                                                            >  1:135859/1‑143 (MQ=255)
                                                              caccacGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCGCCTTTTATTGttt                                                                         >  2:904558/1‑143 (MQ=255)
                                                               accacGCCACAGGTTGCCGACGCGATTTGCAACATTATTTTGCGTTGAGGTCAAACCGGTTTATTTGAACCGCGTCACTGACGCGGTCGTTTTATTCGTTCTTTGCAGTAAATGACCGGCGTGATTCGAACTTGTATTggtgc                                                                        >  2:378628/1‑139 (MQ=255)
                                                               accacGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCCTTTCCCCTTTTATTGTTTc                                                                        >  2:960794/1‑143 (MQ=255)
                                                                     ccACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAACAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTc                                                                  >  2:926385/1‑143 (MQ=255)
                                                                      cacaGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTCTCAGttccgttccg                                                                 >  2:837531/1‑143 (MQ=255)
                                                                           gTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTAACTGACGCGCTTAAATCATACGTTATTTGCAGTAAATAACATGCGAAATTTCAACATCAATTGTTTCCGGTTCgtgtag                                                            >  2:188010/1‑141 (MQ=255)
                                                                            ttGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGtttt                                                           <  2:991698/143‑1 (MQ=255)
                                                                             tGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCTCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGGTTCGTGTTTTa                                                          >  2:570793/1‑143 (MQ=255)
                                                                              gCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTAt                                                         <  1:930190/143‑1 (MQ=255)
                                                                                 gACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCGCCTTTTATTGTTTCCGTTTCGTGTTTTATGGc                                                      >  1:957642/1‑143 (MQ=255)
                                                                                 gACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATGGTTTCCTTTTCGTGTTATATGGc                                                      >  2:1020455/1‑143 (MQ=255)
                                                                                  aCGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTAGTCACCAGCCTGAAAGAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCt                                                     >  1:520522/1‑143 (MQ=255)
                                                                                    gcgATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCttt                                                   >  1:749562/1‑143 (MQ=255)
                                                                                    gcgATTTGCAAAATTATATTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTAACCTTTTATTGTTTCCGTTTCATGTATTATGGCttc                                                   >  2:436026/1‑142 (MQ=255)
                                                                                    gcgATTTGCAAAAGTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCttt                                                   <  2:878556/143‑1 (MQ=255)
                                                                                     cgATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTc                                                  <  1:772502/143‑1 (MQ=255)
                                                                                     cgATTTGCAAAATTATTTCGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTc                                                  <  1:913518/143‑1 (MQ=255)
                                                                                      gATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATgctcttcc                                                 >  1:987849/1‑136 (MQ=255)
                                                                                         ttGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTACTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTa                                              <  2:593252/143‑1 (MQ=255)
                                                                                          tGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTAt                                             <  2:200511/143‑1 (MQ=255)
                                                                                          tGCAAAATTATTTTGCCTTGAGGTCAAACCAGTTCATTCGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTAt                                             <  1:648767/143‑1 (MQ=255)
                                                                                               aaTTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTa                                        <  1:869870/143‑1 (MQ=255)
                                                                                                  tattTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAtt                                     <  1:848117/143‑1 (MQ=255)
                                                                                                       tGCGTTAAGGTTCATCCAGTGTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTGATGGCTTTCCGTATTCTTAATTGTTTa                                <  2:919846/143‑1 (MQ=255)
                                                                                                       tGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTa                                <  1:878553/143‑1 (MQ=255)
                                                                                                        gCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCTTCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTaa                               <  1:648198/143‑1 (MQ=255)
                                                                                                            tAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTa                           <  1:607536/143‑1 (MQ=255)
                                                                                                            tAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTa                           <  1:469135/143‑1 (MQ=255)
                                                                                                              aGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTCATTGTTTAATTTATg                         >  1:289873/1‑143 (MQ=255)
                                                                                                              aGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTTATTTATg                         >  1:30827/1‑143 (MQ=255)
                                                                                                                gTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTTTTTTTTTTTTACAGTAAATAACCTGCGCCATTTTTCGTTTTTTTGTTTCCGTTTCGTGTTTTATGGCTCTCCCTTTTCTTAATTGTTTATTTTATGtt                       >  2:467619/1‑142 (MQ=255)
                                                                                                                gTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTCTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTTTTTAATTTATGTa                       >  2:531857/1‑143 (MQ=255)
                                                                                                                 tCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTaa                      <  2:157990/143‑1 (MQ=255)
                                                                                                                  cAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTATTATTCGTTCTTTGCAGTAAATAACCTGCGTAATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATGGTTTAATTTATGTagc                     >  2:158786/1‑141 (MQ=255)
                                                                                                                            tATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAAttt           <  2:209826/143‑1 (MQ=255)
                                                                                                                              tttGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTg         <  1:463627/143‑1 (MQ=255)
                                                                                                                                 gAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTa      <  1:1059193/143‑1 (MQ=255)
                                                                                                                                  aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTa      <  1:771531/142‑1 (MQ=255)
                                                                                                                                  aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTa      >  2:771531/1‑142 (MQ=255)
                                                                                                                                  aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGACATTTCACCGTTCATTGTTTCCGTTTCGTGTTTTATGGCCTTCCGTATTCCTAATTATTTAATTTTTGTAACACTCACATTTCGTTAc     >  2:215138/1‑143 (MQ=255)
                                                                                                                                     cgcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTAcgcg  >  1:1017969/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTATGTAACATGCAAATTTTGTTACGCG  >  NC_000913/1882887‑1883162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑