Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 124,241 C→T 100% Q409* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913124,2410CT100.0% 103.4 / ‑6.7 47Q409* (CAG→TAG) aceEpyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (27/16);  minor base G (1/1);  total (29/18)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTG  >  NC_000913/124105‑124372
                                                                                                                                        |                                                                                                                                   
tggtCACGATCCGAAGAAAATCTACGCTCCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGCCATGGGCGACGCGGCTGAAGGTAAACTCATCGCGCCCCATGTTa                                                                                                                               >  1:835610/1‑143 (MQ=255)
  gtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTCATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGATGAAGGGAAAAACATCGCGCGCTAGGTTagg                                                                                                                             >  1:316350/1‑141 (MQ=255)
  gtCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAg                                                                                                                             >  1:933249/1‑143 (MQ=255)
    cACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGaa                                                                                                                           >  1:961063/1‑143 (MQ=255)
     aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGcaa                                                                                                                          >  1:582311/1‑143 (MQ=255)
     aCGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACAGCGCGCACGAGGTTAAGaaa                                                                                                                          >  2:25339/1‑143 (MQ=255)
       gATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCACGCACTAGGTGAAGAAAAt                                                                                                                        >  2:286133/1‑143 (MQ=255)
       aaTCCGAACAAAACATCCGCCGCATTCAAGACAGCGCAGGAAACCAAAGGCACAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAAt                                                                                                                        <  2:836170/142‑1 (MQ=255)
        aTCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATg                                                                                                                       >  1:756409/1‑143 (MQ=255)
               gaaAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTCGGGTAAGAAAGTGAACATgg                                                                                                                >  1:907508/1‑143 (MQ=255)
                   aTCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTAAACACACTGAACATGGACgg                                                                                                            >  2:378618/1‑143 (MQ=255)
                     cTACGCGGCATTCAAGAAAGCGCAGGAAACTAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGgtg                                                                                                          <  1:35262/143‑1 (MQ=255)
                        cGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGc                                                                                                       >  1:878846/1‑143 (MQ=255)
                        cGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGc                                                                                                       >  1:665909/1‑143 (MQ=255)
                          cTGCATTGAAGAAAGCGCAGGACACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGt                                                                                                     <  2:950933/143‑1 (MQ=255)
                          cTGCATTCAAGAAAGCGCAGGAAACCACAGGCAAAGCGACAGTAATCCTTGCTCATACCATTACAGGTTACGGCCTGGGCGACGCGGCTGAAGGTAGAAACATCGCGCCCTAGGTTAAGAAAATGAACATGGACGGTGTGCGt                                                                                                     >  1:78495/1‑143 (MQ=255)
                                  aaGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAGCATGGAGGGTGTGCGCCATAGCcg                                                                                             >  2:176107/1‑143 (MQ=255)
                                     aaaGCGCAGGGAACCAAAGGCAAACCGACAGTACGCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAGCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGa                                                                                          <  2:537213/143‑1 (MQ=255)
                                         cgcAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACAATGAAAGGTTACGGCATGGGCGACGCGGCTGAAGGGAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGt                                                                                      >  2:1021533/1‑143 (MQ=255)
                                           cAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGctt                                                                                    >  2:205588/1‑143 (MQ=255)
                                            aGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTc                                                                                   <  2:216417/143‑1 (MQ=255)
                                                          aaaGCGACCGTAATCCTTGCTCATACCATTAAAGGTTACGGCGCGGGCGACGCGGCTGAAGGTCAAAACATCGCGCACTAGGGTAAGAGAGTGAACATGGACGGTGTGCGGCATACCCGCGACCGCATCAAAATGCCggcggc                                                                     >  2:990514/1‑139 (MQ=255)
                                                             gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGATGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATACGCCGGGGGCTGa                                                                  >  2:1083681/1‑143 (MQ=255)
                                                             gCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGGTTCAATGTGCCGGGGTGTGa                                                                  >  2:943229/1‑143 (MQ=255)
                                                                  aGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGCAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGGCGGTGTGCGTCATATCCGCGGCCGTTTCTATGTCCTGTTGTCTGATGcgt                                                             >  1:311328/1‑141 (MQ=255)
                                                                  aGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAg                                                             >  1:622385/1‑143 (MQ=255)
                                                                   gTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGa                                                            <  2:261656/143‑1 (MQ=255)
                                                                    tAATCCTTGCTCATACCATTAAGGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGat                                                           <  1:991242/143‑1 (MQ=255)
                                                                    tAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTCATGCacag                                                           <  2:460188/143‑4 (MQ=255)
                                                                    tAATCCTTGCTCATACCATTAAAGGTCACGGCATGGACGACGCGGCAGAAGGTAATAACACCGCGCCCTAGGTTAAGAACATGAACATGGACGGTGTGCGCCATATCAGCCACGGTCTCAATGTGCCGGTCTCCGCCGCAGat                                                           >  2:823603/1‑143 (MQ=255)
                                                                       tCCTTGCTCATACCATTACAGGTTACGGCATGGGCGACGTGGCTGACGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATc                                                        <  1:286133/143‑1 (MQ=255)
                                                                           tGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAGCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGaaa                                                    <  2:756409/143‑1 (MQ=255)
                                                                                        aaaGGTTACGGCATGGGCGCCGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACAt                                       <  1:378618/143‑1 (MQ=255)
                                                                                            gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGCTTCAATGTGCCGGCGTCTGATGCAGATATCGAAAAACTGCCGTACATcccc                                   >  1:859236/1‑143 (MQ=255)
                                                                                            gTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGAGAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTCTCAATGAGCCGGTGACTGATGCAGGTCTCGAACAACTGACGTACAGCAcg                                   >  2:1007558/1‑142 (MQ=255)
                                                                                                   cATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCCTACATCACCTTccccg                            >  1:943984/1‑141 (MQ=255)
                                                                                                    aTGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCG‑AAAACTGCCGTACATCACCTTCCCGGaa                          <  1:244168/143‑1 (MQ=255)
                                                                                                         ggACGCGGCTGAAGGTAAAAACATCGCGGACTAGGTCAGGAGGATGTTCGTGGGCGGTGGGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGtt                      <  2:1040999/142‑1 (MQ=255)
                                                                                                           aCGCGGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCt                    >  1:465330/1‑143 (MQ=255)
                                                                                                             gcgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTGCATCACCTTCCCGGAAGGCTCTGa                  >  1:911226/1‑143 (MQ=255)
                                                                                                             gcgGCTGAAGGTAAAAACATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTCCTGa                  >  2:1070251/1‑143 (MQ=255)
                                                                                                              cgGGTGGAGGTAAAGAGACGGCGCACTAGGGTAAGAAAATGAGCATGGCCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAACACTCCCGTACATCACCTTCCCGGAAGGTTCTGaa                 <  2:347722/143‑1 (MQ=255)
                                                                                                                     aGGTAAAACCATCGCGCACGAGGTTAAGGAAGTGAACAGGGCCGGTGCGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATa          <  1:498329/143‑1 (MQ=255)
                                                                                                                           aaaCATCGCGCACCAGGTGAAGAAAGTGAACATCGACAGTGTGCGTCATATCCGCGTCCGTTTCAATGTGGCGGTGCCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCtctc    <  2:316350/143‑1 (MQ=255)
                                                                                                                           aaaCAGCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATc    <  2:933249/143‑1 (MQ=255)
                                                                                                                            aaCATCGCGCACTAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTCCATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCt   >  1:38011/1‑143 (MQ=255)
                                                                                                                             acctCGCCCACTAGGTTAAGAAAAGGAACAGGGACGGTGTGCGTCATAGCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTg  <  1:341108/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
TGGTCACGATCCGAAGAAAATCTACGCTGCATTCAAGAAAGCGCAGGAAACCAAAGGCAAAGCGACAGTAATCCTTGCTCATACCATTAAAGGTTACGGCATGGGCGACGCGGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTG  >  NC_000913/124105‑124372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑