| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,368,101 | T→C | 100% | A21A (GCT→GCC) | arnA → | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,368,101 | 0 | T | C | 96.3% | 99.1 / ‑5.2 | 51 | A21A (GCT→GCC) | arnA | fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (27/19); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGGGTG > NC_000913/2367963‑2368242 | tCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTCCCACGATATGCGCTGCCTCGGTATCGAAGCCCCGCTGGccgccg > 2:270114/1‑143 (MQ=255) cacCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAATACTGTTGTTTTTGCCTAACAAGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTa < 1:1059226/143‑1 (MQ=255) acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCACGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTag > 2:1014907/1‑142 (MQ=255) acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACTGTGGTTGTCCCCAACCACGATATGGGCTGCCTCGGTATTGTCGCCCTGCTGGCCGCCCGtttc > 1:519839/1‑141 (MQ=255) acCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGACGCCCTGCTTGCCGCCGGTTAc > 1:857921/1‑143 (MQ=255) cGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCATACCACGATATGGGATGCCTCGGTATTGAAGCCCTACTGCCCGCCGCTTACgg > 2:162711/1‑142 (MQ=255) cGATGTCCGCGCCCGCCCCCGCTATTTTGTTAAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATTGGCTGCTTCGGTATTCAACACATGCTGGCGCCCAGTTActa > 2:556460/1‑141 (MQ=255) tCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCAGTCCATGCAGCAAGGAAAATGAATAGTGAAAACAGTCGTTTTTGAGTACCACGATAGGGGATGGCTCGGAAATGAAGCCTGGGCGGCCGCCGGTTGCGGAAtga > 2:1030865/1‑141 (MQ=255) ccGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGATTACTGTTGTTTTTGTTTATTTTGATATGGGATGACTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAg < 1:1020163/143‑1 (MQ=255) cgtgcccgcctCGGCTATTGGGTGTAGGAGGTTAGCCGTCCATCCAGCAAGGAAGATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAgc < 2:771747/140‑1 (MQ=255) ccgcccCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGCGTCCCTGCTGGCCGCCGTCTGCGTACTTAGCGCCAt > 1:837036/1‑143 (MQ=255) ccccccGTATTTTGTTCGGGAGGTTTGCCGGCCAGCCAGCAAGGAAAATGGATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCAtttt < 2:1078757/143‑1 (MQ=255) cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACTGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTCACGAGATTAGCGCCATTCTTa > 1:1073314/1‑143 (MQ=255) cccGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCACAATTTTTa > 1:557919/1‑143 (MQ=255) gCAAGTTATCCGTACATCCAGCAAGGAAAATGAATAATGAAAACCGTCGGTTTTGCCTACCACGATATGGGATGCCTAGGTATTGAAGCCCTGCCGAACATCGCGTACGAAATCAGCGCCATTTTTACGCATACTGATAATcc > 2:497374/1‑143 (MQ=255) tgTTCTCCGTCCTTCCAGCAAGGAAACTGTATAATGACAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCgg < 2:164252/142‑1 (MQ=255) atttATCCGTCCATCCAGCAAAGAAAATGATTAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCgg < 2:1072268/141‑1 (MQ=255) aGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTAAGAAATTAGCGCCATTTTTACCCATACTGATAAGCGCgg > 2:46050/1‑143 (MQ=255) aGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGATACGAAATTAGCGCCCTTTTTACCCATACTGATAATCCCgg > 2:144113/1‑143 (MQ=255) gTTATCAGTCTATCCAGCAAGGAAAATGAATAATGAAATCCGTCTTTTTTGTCTACCACGATATGTGATGTTTCGGTATTTAAGCTCTGCTGGCAGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGt < 1:640201/143‑1 (MQ=255) cGTCCATCCAGCAAGGAAAATGAATAATGACAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGaaaaa < 2:746028/143‑1 (MQ=255) atccaGCAAGGAAAATGGATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAATCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCtt < 2:409388/143‑1 (MQ=255) aGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTTGTGCCACTGTTTCTTATCCTGATAATCCCGATGAAAAAGCCATGTAt > 1:516275/1‑143 (MQ=255) gCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTAACACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGACCGACGGGTACGAAATTAGCGCCGTTTTTACCCATACTGATAATGCAGGTGAAAAAGCCtttaacg > 2:1075526/1‑139 (MQ=255) gAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCg > 1:531197/1‑143 (MQ=255) aaaaTGAATGATGCAATCCGTCGTTTGGGCCTCCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCAATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCgg < 1:685885/143‑1 (MQ=255) aTGAATAATGAAAACCGTCGTTTTTGCATACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTTTTTTCTTTTTACTTATTTAGTGCCATTTTTACCAAAACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTgg < 1:434828/143‑1 (MQ=255) gAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAACATTTTATGGTTCGGTGGcc > 2:683911/1‑142 (MQ=255) gaccaCCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTTGTGCCATTTTTACCCATTCTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTgg < 1:598434/139‑1 (MQ=255) taaaTCGTCGGTTTTGCCGACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTATGAAATTAGCGTTTTTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTggc < 1:658946/142‑1 (MQ=255) aaaaCCGTCGTTTTTGCCTACCACGATATGGGACGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGATATTAGCGCTATTTTTACCCAGTCTGATAAGCCCGGGGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTggc < 1:736111/143‑1 (MQ=255) gtcgtcttTTTTGTCTACCACGATACGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAAGTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTggcggc < 1:56361/141‑1 (MQ=255) ccGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCATTTATGGTACGGTGGCTCGTCTGgcggcg > 1:755348/1‑143 (MQ=255) gtcgtTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGa > 2:6170/1‑143 (MQ=255) tttgGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCTATTTTTACCCATACTTATAATCTTGGTGAAAAAGCAAATTATGGTTCGGTGGCTCGTCTGGCGGCGGAAagag < 1:582489/143‑1 (MQ=255) ttGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCAGCGTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATAGTTCGGTGGCTCGTCTGGCGACGGAAagagac > 2:826161/1‑141 (MQ=255) aCCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCg < 2:521679/143‑1 (MQ=255) cGATATGGGATGCCTCGGTATTGAAGCCCGGCGAGCCGCCGGTGACGAAATTGGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAGAGCCTTTTATGGTTCGGTGGCTCGTCGGGCGGAGGAAAGAGGCATGCAGGttt > 2:839349/1‑143 (MQ=255) atGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAACGACTTTTATGGTTCGGTGGTTCGTCTGGCGGCGGCCACAGGCATTCCGGTTTCTgc > 2:736878/1‑143 (MQ=255) tGGGATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATgcg > 1:1011106/1‑143 (MQ=255) gggATGCCTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGGTTATgagc > 1:342339/1‑143 (MQ=255) gATGCCTCGGTAGTTTAGCCTCGCTGTCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTGTTATGGTTCGGTGGCTCGTCTGGCGGCTGAAAGAGGCATTCCGGTTTATGCGCCg < 2:90800/143‑1 (MQ=255) cTCGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCTGTGAAAAAGTCTTTTATGGTTGGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATaa < 1:640213/143‑1 (MQ=255) cGGTATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGGTTATGCGGCGGATACCg > 2:614510/1‑143 (MQ=255) tATTGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTGCGGGTTAGGCGCCGGAGCACGtgc > 2:442674/1‑141 (MQ=255) tGAAGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGACGACGGAAAGAAACATTCCGGGTTATGCGCCGGATGAGGTTaagc > 2:847730/1‑141 (MQ=255) gAAGCCCTGCTGGCCGCCGGTTACGAAAATAGCGCCATTTTTACCCATACTGATAATCCCGGTGGAAAAGCCCTTTATGGTTCGGTGGCTCGTCTGGCGGCGCAAAGAGTCATTCCGGTTTATGCGCCGGATAACGATAaaca > 1:848976/1‑143 (MQ=255) aatcccTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcat < 2:1011106/140‑1 (MQ=255) aaccccTGCTGGCCGCCCGTGACTAAATTAGCGCCATTTTTACCCATACTGATATTCCCGGTGAAAAAGCCTTTTCTGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcat < 2:1115487/140‑1 (MQ=255) aaGCCCTGCTGGCCGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAatcat > 1:441482/1‑143 (MQ=255) ccTGCTGGCCGCCTGTTACGAAATTAGCGCCTTTTTTACCCCTACTGATAACCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGc < 2:1027778/143‑1 (MQ=255) ctggtgGCCGCCGGTTACGAAATTAGGGCCATTTTTTCCCAGACTGATAATCCCGATCACAAAGACGTCTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCt < 2:237359/143‑1 (MQ=255) gctggcagCCGGTTACTGAAGTAGCGCCATCTTTACTCATACTGATAATCCCGGTGCTATAGCCTTTTCTCAACCTGTCGCTTGTATTGCATCGGAAAGAGGCATTCCGGTCTATGCGCCGGATAACGTTAATCATCCGCtgt < 2:1104329/143‑1 (MQ=255) ctggccgCCGGTGACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTTTTTTGTCTGGCGGCGGAAAGAGGCATTCCGGTTGAGGCGCCGGATAACGTTAATCATCCGCtgtg < 1:12977/143‑1 (MQ=255) ctgCCAGTGACGAAATTTGCGCCATTCTTCCCCATAGTGCCAAGCCCGTTGAAAAAGCCTGTTATGGTTCGGGGGCTCGTGTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTgtgggtg < 2:335018/143‑1 (MQ=255) | TCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGCGCCATTTTTACCCATACTGATAATCCCGGTGAAAAAGCCTTTTATGGTTCGGTGGCTCGTCTGGCGGCGGAAAGAGGCATTCCGGTTTATGCGCCGGATAACGTTAATCATCCGCTGTGGGTG > NC_000913/2367963‑2368242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |