Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,383,956 A→T 8.2% intergenic (+32/+39) elaD → / ← yfbK protease ElaD/IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,383,9560AT 8.2% 137.9 / 4.6 49intergenic (+32/+39)elaD/yfbKprotease ElaD/IPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base A (19/26);  new base T (2/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

TGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACT  >  NC_000913/2383817‑2384095
                                                                                                                                           |                                                                                                                                           
tGTTATTTAGATAAATATGGCGATGCAAATAGCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                          <  1:863726/143‑1 (MQ=255)
tGTTATGTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                          <  1:894469/143‑1 (MQ=255)
  ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATg                                                                                                                                        >  1:1024209/1‑143 (MQ=255)
      ttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGa                                                                                                                                    <  2:521091/143‑1 (MQ=255)
        aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACt                                                                                                                                  <  2:48554/143‑1 (MQ=255)
        aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACt                                                                                                                                  <  2:473703/143‑1 (MQ=255)
        aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAA‑ACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                 <  2:972900/143‑1 (MQ=255)
        aGATAAAGATGGCGATGCAAATATCAAGCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACt                                                                                                                                  <  2:870809/143‑1 (MQ=255)
         gATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                 <  2:341275/143‑1 (MQ=255)
          aTAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAAGAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTCTTGATTAATTCGACCAGTCAGCAATTATGCGGACTgc                                                                                                                                >  1:119405/1‑142 (MQ=255)
                       tgtaaaTATCATTCATTATTATAGAAACTTATAAATAATACACTCACAACCCAAAAATAGAGCATCCTGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGttt                                                                                                                   <  2:6308/140‑1 (MQ=255)
                       tGCAAATATCAATCATTATTATATAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGttt                                                                                                                   <  2:961427/143‑1 (MQ=255)
                             tatCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaaa                                                                                                             >  1:793712/1‑143 (MQ=255)
                              atcaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCTAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAAc                                                                                                            <  2:646289/143‑1 (MQ=255)
                                  atcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAAAGTGAGTTAACATCATTGTTAATTAATTCGACCAATCAGCAAATCTACTAACTGGACTAACATAGTTTGATaaagcatc                                                                                                        >  2:594736/1‑138 (MQ=255)
                                            taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactgact                                                                                              >  1:243008/1‑143 (MQ=255)
                                              gAAACTTAGAAATAAAACACTCACTACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCActgactga                                                                                            <  1:380961/143‑1 (MQ=255)
                                                   ttAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAGTTCGACAAGTCAGCAAATATGCTGACTGGTCGAACATAGATTGCTAAACAGTCAGCACTGAAGGATgtca                                                                                       >  2:79075/1‑143 (MQ=255)
                                                   ttAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTGAGCAAATATACTGACTGGTCGAACATAGTTTGCAAAACAGTCATCACTGACTGATgtca                                                                                       >  2:556543/1‑143 (MQ=255)
                                                     aGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                     >  2:64691/1‑143 (MQ=255)
                                                         aTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTcaca                                                                                 <  1:161238/143‑1 (MQ=255)
                                                          tAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGGGTTAATATGTTTGGTCACTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCacac                                                                                <  1:1125384/143‑1 (MQ=255)
                                                               cacTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGGGAGTTAACATCGTTGTTGATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATcc                                                                           <  1:822800/143‑1 (MQ=255)
                                                                  tCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCTTCCGcc                                                                        >  2:152919/1‑143 (MQ=255)
                                                                   cacaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCa                                                                       <  2:939100/143‑1 (MQ=255)
                                                                         ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCaa                                                                 <  1:232865/143‑1 (MQ=255)
                                                                                           gCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGATTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCg                                               >  2:613227/1‑143 (MQ=255)
                                                                                            cAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGc                                              >  1:15078/1‑143 (MQ=255)
                                                                                             aaaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGcc                                             <  1:64691/143‑1 (MQ=255)
                                                                                               aaGAGTGAGTGAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTGTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                           <  2:854696/143‑1 (MQ=255)
                                                                                                 gagtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGt                                         <  2:1024209/143‑1 (MQ=255)
                                                                                                   gtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTaa                                       <  2:1115503/143‑1 (MQ=255)
                                                                                                      agtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAccc                                    <  1:604602/143‑1 (MQ=255)
                                                                                                        ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCACTCGGGCGACTAAATTCCGCCCTGTAAccccg                                  >  2:732701/1‑141 (MQ=255)
                                                                                                           aCATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTgg                               <  1:814206/143‑1 (MQ=255)
                                                                                                              tcagTGTTAATTAATTCGACCAGTCAGCAAATAGGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATc                            <  1:676805/143‑1 (MQ=255)
                                                                                                                atTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCtt                          <  2:888030/143‑1 (MQ=255)
                                                                                                                        ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCGGTAACCCTGTGGACCTTCCCCttcc                  >  1:482163/1‑141 (MQ=255)
                                                                                                                        ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGACAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCtttt                  >  2:638361/1‑143 (MQ=255)
                                                                                                                        tatgttCGACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACCGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCCGGCGAATAAATTTCGCCATGTAACACTTCGGATATTCCCCtcta                  >  2:822869/5‑140 (MQ=37)
                                                                                                                        tatgttCGACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCtttt                  >  1:969520/5‑143 (MQ=255)
                                                                                                                               gACCACTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgct           <  2:932470/143‑1 (MQ=255)
                                                                                                                                 ccAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGa         <  1:191490/143‑1 (MQ=255)
                                                                                                                                   aGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGc       >  1:873445/1‑143 (MQ=255)
                                                                                                                                   aGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGc       >  1:760223/1‑143 (MQ=255)
                                                                                                                                   aGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTCATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGc       <  2:577270/143‑1 (MQ=255)
                                                                                                                                      cAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGACCTTCCCCTTTTGCCTGCTGAGCCCa    >  2:771932/1‑143 (MQ=255)
                                                                                                                                        gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACt  >  1:853375/1‑143 (MQ=255)
                                                                                                                                        gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTCCCTGCTGAGCCCACt  >  2:726930/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACT  >  NC_000913/2383817‑2384095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑