Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,637,444 A→G 7.5% intergenic (‑88/+30) der ← / ← bamB 50S ribosomal subunit stability factor/outer membrane protein assembly factor BamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,4440AG 7.5% 148.4 / 2.6 63intergenic (‑88/+30)der/bamB50S ribosomal subunit stability factor/outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base G (3/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

ATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGG  >  NC_000913/2637302‑2637579
                                                                                                                                              |                                                                                                                                       
acaaCGTCGATTCTCCTACGTTAGGGCGCCCTACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTCATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGa                                                                                                                                         <  1:421195/141‑1 (MQ=255)
  aaCGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGc                                                                                                                                       <  1:366097/143‑1 (MQ=255)
      tGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAGGCCTCGTTTTTATAAAGCATCAGACAACGCACGCTATCTTCGCGTCGTTGTTGAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGtc                                                                                                                                   >  2:750088/1‑142 (MQ=255)
         aTTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGCACCACGTCTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGTTTTc                                                                                                                                <  1:894406/143‑1 (MQ=255)
            ttCCGACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCAGCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                             <  1:318723/143‑1 (MQ=255)
                tACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTGTAAAGCCTCATTTTTATAAATCATCAGACAACGCCCGCTATATTCGCGTCGTTGTTAAAAACAGGAACACGGCCACTGTCCCGGCGCACTTGTCGAAGTGa                                                                                                                         >  1:1112124/1‑143 (MQ=255)
                    ttAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGa                                                                                                                     >  1:1052338/1‑143 (MQ=255)
                     tAGGGCGCCCGACAAGCGCGACCACAGTTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc                                                                                                                    <  2:633395/143‑1 (MQ=255)
                      ggcgggcCCGACGAGCGCGACCACAGGTACCATGTTTAAAGCCTGATTTTTTTAAATCAGCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAGCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACa                                                                                                                   <  2:482167/138‑1 (MQ=255)
                      aGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATCTTCGCGTCGTTGTTAATCAAAGGATTTCGTTCCCGGGCCAGGAGCCGTTTTCAAAGTGAACGACa                                                                                                                   <  1:215402/143‑1 (MQ=255)
                       gggCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACag                                                                                                                  <  1:598928/143‑1 (MQ=255)
                        ggggcCCCACAAGCGCGACTACATGTACCATGTTTAAAGGTTCATTTTTCTAGCTCCTCAGACAACGCTCGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga                                                                                                                 <  2:395105/140‑1 (MQ=255)
                          cgcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAgaga                                                                                                               <  2:763858/143‑1 (MQ=255)
                                        gtccACAGCTACCCTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTaa                                                                                                 <  1:749888/141‑1 (MQ=255)
                                        gACCACAGGTACCACGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTaa                                                                                                 <  1:358583/143‑1 (MQ=255)
                                         aCCACAGGTACCATGGTTAAAGCCTCCTTTGTATAAAGCATCAGACAACGCACGCTATATGCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAAt                                                                                                <  2:230963/143‑1 (MQ=255)
                                          ccACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATGTTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATa                                                                                               <  2:476408/143‑1 (MQ=255)
                                            acaGGTACGATGTTTCAAGCCTCATTTTTATAAATCATCAGGCAACGCACGCTATATGCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                             <  2:76999/143‑1 (MQ=255)
                                              aGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAGCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGt                                                                                           >  1:566565/1‑143 (MQ=255)
                                                  aCCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                       <  1:622778/143‑1 (MQ=255)
                                                  aCCATGTTTAAACCCTCATTTTTAAAAATCATCAGACGACGCACGCTCTATTCGCGTCGTTGTTACAACTAGTAAAACGGCCCCTATCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                       <  2:919039/143‑1 (MQ=255)
                                                     aTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAAAGAGACGATTAACGGGTAGTAGAGTATCCGGt                                                                                    >  2:474474/1‑143 (MQ=255)
                                                      tGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGtt                                                                                   >  2:291930/1‑143 (MQ=255)
                                                        tttAAAGCCTCATTTTTATAAATCATCAGTCAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTcc                                                                                 <  2:756059/143‑1 (MQ=255)
                                                         ttAAAGCCTCATTTTTATCAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCg                                                                                <  1:364899/143‑1 (MQ=255)
                                                               ccTCATTTTTATGAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCTTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCtttt                                                                          <  2:43254/143‑1 (MQ=255)
                                                                 tCATTTTTATAAGTCATCAGACAACGCACGCAATCATCACGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGc                                                                        <  1:695002/143‑1 (MQ=255)
                                                                 tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGc                                                                        >  1:875676/1‑143 (MQ=255)
                                                                 tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGc                                                                        >  1:896131/1‑143 (MQ=255)
                                                                 tCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTATTAAAAACAAGAAAACGGCATCTGTCCATGAGCCGTTTTAAAAGTGAACGACAAAGACAATTAACGTGTAATAGAGTACACGGTTCAGACtttatc                                                                        >  2:682261/1‑140 (MQ=255)
                                                                   aTTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCt                                                                      >  1:534261/1‑143 (MQ=255)
                                                                    tttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTg                                                                     <  2:473922/143‑1 (MQ=255)
                                                                      gttATAAGTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGa                                                                   <  1:730419/142‑1 (MQ=255)
                                                                       ttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGAt                                                                  <  2:440637/143‑1 (MQ=255)
                                                                        tataAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACACACACGATTAACGTGTAATAGAGTACACGGTTACGACTTTTTCCTgcgtc                                                                 >  1:1100893/1‑139 (MQ=255)
                                                                          taAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcag                                                               <  2:1052338/143‑1 (MQ=255)
                                                                          taAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCCGGTACCAGGATcag                                                               >  2:123905/1‑143 (MQ=255)
                                                                          taAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTAAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTGCCCGGTTCCGTCTTTTGCCTGGATcag                                                               >  2:800186/1‑143 (MQ=255)
                                                                           aaaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGACTTTTACCTGGCTcagc                                                              >  2:99447/1‑143 (MQ=255)
                                                                            aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTGCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTGATAGAGCACACGGATCCGTCTCTTGCCTGGAtacgca                                                             >  2:1031667/1‑138 (MQ=255)
                                                                               catcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGGTTTCAAAGTGAACGACAGAGACGAGTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGtc                                                          >  1:430523/1‑142 (MQ=255)
                                                                                atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGttt                                                         <  1:1096134/143‑1 (MQ=255)
                                                                                 tcaGACAAAGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTg                                                        >  1:218961/1‑143 (MQ=255)
                                                                                  caGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGc                                                       >  1:15976/1‑143 (MQ=255)
                                                                                    gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGGACACGGTTCCGTCTTTTGCCTGGAAGAGCAGTTTGCCg                                                     >  1:579852/1‑143 (MQ=255)
                                                                                         cgcaccaTACATTCGCGTCGTTGTGCAAAACAATAAAACGTCCCCTGTCCAGGATCCGTTTTCAAAGTGAACGACCGAGACGATTAACGTGTAATGGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGc                                                <  2:79196/143‑1 (MQ=255)
                                                                                             cgcTATATGCGCGTCGTTGTTTATAACAGGAAAACGGCCCCTGTCCAGGGGCCATGTTCAAAGTGAACGACTGCGACGATTAACGTGTAATAGAGTGCACGGTTCCGTTTTTTGCCTGGATCAGCAGTTTGTCGTCAGCGGCa                                            <  2:616039/143‑1 (MQ=255)
                                                                                              gcTATTTTTGCGTCTTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGGTACCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCaa                                           <  1:431110/143‑1 (MQ=255)
                                                                                               caatacgcgcgCCGTTGTGAAAAACAGGAACACGGCCCCTCTCCAGGAGCCGTTTCCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGTATCAGCAGTTTGCCGTCAGCGGCAAc                                          <  2:658389/136‑1 (MQ=255)
                                                                                                    ttCGCGTCGCTGTCAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACTTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGtt                                     <  1:1013048/143‑1 (MQ=255)
                                                                                                     tCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTc                                    >  1:574611/1‑143 (MQ=255)
                                                                                                             ttgttAAAAACAGGAAAACGGCCCGTGTGCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGGCTggc                            >  2:992053/1‑142 (MQ=255)
                                                                                                                ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGCTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAAc                         >  2:253202/1‑143 (MQ=255)
                                                                                                                  aaaaaCGGGAACACGGCCCCTGTCCAGCAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGGCAGCGGCAACCGGTTCAGTCTGGAAACCg                       <  2:208431/143‑1 (MQ=255)
                                                                                                                      aCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGCGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCCGCAACCGGTTCAGTATGGCAACCGGGAc                   >  2:502747/1‑143 (MQ=255)
                                                                                                                      aCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCGGCGGCAACCGGTTCAGTCTGGAAACCGGAAc                   >  1:595691/1‑143 (MQ=255)
                                                                                                                      aCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAAAGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGCTCAGCAGTTTGCCGTCAGACGCAACCGGTTCAGTCTGGAAAACAGaaa                   >  2:342473/1‑142 (MQ=255)
                                                                                                                       tttgAAAACGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACCCGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGCCAGCGGCAACCGGTTCCGCCCGGAAACCGGCAcg                  >  2:1071575/4‑142 (MQ=255)
                                                                                                                            aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTTGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCACACTCGAAACCGGAACTATCag             >  1:592110/1‑142 (MQ=255)
                                                                                                                            aaaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCACCCGGTTCAGTCTGGCAACCGGAACGATCaa             >  1:645275/1‑143 (MQ=255)
                                                                                                                                 gCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAATACACGGTTCCGTCTTTTGCCTGGATCAGCAGATTGCCGTCAGCGGCCACAGGTTCAGTCTGGAACCCGGAACTATCAACtttc        >  2:133010/1‑142 (MQ=255)
                                                                                                                                  ccccTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGACACCGGAACTAGCAACttttt       >  1:443760/1‑143 (MQ=255)
                                                                                                                                   cccGGTCTAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTg      <  1:1036608/143‑1 (MQ=255)
                                                                                                                                       gTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAACCCGGGACTCTCAACTTTTTGCTgg  >  2:545592/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                       
ATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGG  >  NC_000913/2637302‑2637579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑