Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,661,821 C→G 100% C104S (TGC→TCC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,8210CG100.0% 141.7 / ‑8.3 46C104S (TGC→TCC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (18/26);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTAC  >  NC_000913/2661690‑2661960
                                                                                                                                   |                                                                                                                                            
tcgcgGCGTGATTATGCTGTCGCCTTGACACGTCCAGCACTTGCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgccg                                                                                                                                   <  2:537260/142‑1 (MQ=255)
gcgcGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTGAAGTAAGGTTGTTGAGAAAACCGGTGAGACGGTCGCTCCAATCCCGCCACCGCGCTCGGGGCCGGCATCTCTcgccc                                                                                                                                   >  1:741310/1‑142 (MQ=255)
 cgcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATcgccgc                                                                                                                                  <  1:724008/143‑1 (MQ=255)
    gtcgtGAGTATGCTGACGACCAGACACATCCAGCACGTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAACCCGGTGAGACGGGCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCt                                                                                                                               <  1:318945/143‑1 (MQ=255)
             atccTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccgc                                                                                                                      <  2:12718/140‑1 (MQ=255)
              tGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAgccgcc                                                                                                                     >  1:779110/1‑143 (MQ=255)
                 tGACGACCAGACACATCCAGCACTTCGAGGGTTATCACCACCACGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAAGCACGCCGCAGCGCGTGGGTCAGGGACTTATCGCCGCCCTGGCAGCCGCCtac                                                                                                                  >  2:928498/1‑141 (MQ=255)
                 tGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCttt                                                                                                                  >  2:897862/1‑143 (MQ=255)
                  gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTa                                                                                                                 >  2:1112423/1‑143 (MQ=255)
                      acCAGCCACATCCAGCACTTCCTCGGTATTAACCATTTCGCCTAAATTAATATTGTTTAGAAAACCGGTGAGATGGTCTCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCt                                                                                                            <  1:362537/143‑1 (MQ=255)
                      acCAGACACATCCAGCACTTCCTGGTTATTAATCGGTTCGTTTAGTGTAATGTTGTTGAGAATTACGTTGAGATGGTGGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCt                                                                                                            >  1:21131/1‑143 (MQ=255)
                       ccATACACACCTAGCTCTTCCTCGTTCCTAACCAGTTTGTCGAATGTACTGTTGTTGAGAATACCGGTGAGACGGTTCCTCAAATCACGCCACAGCGCGTGGGTCATGCATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTg                                                                                                           <  2:1126951/143‑1 (MQ=255)
                         ccccccaTCCCGCTCCTCCTGGTTGTTAACCAGTTCGCCTACAGGCATGTTGTTGAGCCAGCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGAc                                                                                                         <  2:338179/138‑1 (MQ=255)
                         aGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAATCCGGTGAGTCGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATGGATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGAc                                                                                                         <  1:379659/143‑1 (MQ=255)
                           acacATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACaa                                                                                                       >  2:977819/1‑143 (MQ=255)
                                 ccAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGAGCGTGGGTGAGGGATTTATCGCCGCACTGGCAGCCGCCTTTCC‑GCTGACACCGGGTg                                                                                                 >  1:395395/1‑143 (MQ=255)
                                  cAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGAgg                                                                                                >  1:1101044/1‑143 (MQ=255)
                                     cACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCAt                                                                                             <  2:871824/143‑1 (MQ=255)
                                        ttCCTGGTGATTAACCAGGTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTa                                                                                          <  2:391861/143‑1 (MQ=255)
                                            tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGa                                                                                      >  1:942601/1‑143 (MQ=255)
                                            tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGACAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑ACTGACAACGGCTGGCATCTACCGa                                                                                      >  2:890509/1‑143 (MQ=255)
                                              gTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGCtt                                                                                    >  2:817266/1‑143 (MQ=255)
                                              gTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGAtt                                                                                    >  2:326595/1‑143 (MQ=255)
                                               ttattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACGGCGCGTGCGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTc                                                                                   <  1:422980/143‑1 (MQ=255)
                                               tgattaACCAGTTCGACAAAAGCAATGATATTGAGAATAAAGGAGAGAACGTCGAACAAAAAACGCTCAAGCGCGTGGGTCAGGGATTTATCGCCGCCCAGGCAG‑GGCAAACACTGCTGCAAACGGGTGGCATCTACAGATTc                                                                                   <  2:21131/141‑1 (MQ=255)
                                                    caccgggtCGCCTAAAGTAATGTTGTTGAGAAACCCGGTGAGACGGTCTCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCaa                                                                              <  2:930157/136‑1 (MQ=255)
                                                      ccAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGCTCGCTCAAATCACGCCACAGCGCGTGGGACAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACCACGGGTGGCCTCTACAGCCTCGTCACCg                                                                            >  2:109941/1‑143 (MQ=255)
                                                        aGTTCGCCTAAAGTAATGTTGTGGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGgc                                                                          <  1:652382/143‑1 (MQ=255)
                                                         gtacGCCTAAGGTAAGGTTGGTGAGTAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCTGGGATTTATCGCCGCCCTGGCAGCCGCCTCTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg                                                                         <  2:727694/140‑1 (MQ=255)
                                                                   aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACtt                                                               <  1:311073/143‑1 (MQ=255)
                                                                    gTAATGTTTGTGCGAACTCCGGTGACAGGGTCGCCCCCATCACGCCACAGCGCCTGGTTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGCCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                                              <  2:522248/143‑1 (MQ=255)
                                                                         gttgttGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCaa                                                         <  1:1046004/143‑1 (MQ=255)
                                                                            gttgAGACCACTGGGGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg                                                      <  1:697477/143‑1 (MQ=255)
                                                                               gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGa                                                   <  1:181081/143‑1 (MQ=255)
                                                                                 gaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtg                                                 >  1:495939/1‑143 (MQ=255)
                                                                                    cacgggTGGGACGGTCGCTCAGATCGCGCGGCGGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCGTTAC‑CCTGACAACGGGTGGCATCGACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctg                                              <  2:33034/139‑1 (MQ=255)
                                                                                       cGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCATGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGAGctgctgctg                                           >  2:112217/1‑143 (MQ=255)
                                                                                             gaCGGTCGCTCCAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCt                                     <  2:710396/143‑1 (MQ=255)
                                                                                               cGGTCGCTCAACTCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt                                   <  2:354501/143‑1 (MQ=255)
                                                                                                 gTCGCTCAAATGACTCGCCAGCGCGTGGGTCAGGGATTGATCGCGGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTCCTGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc                                 <  2:1074917/143‑1 (MQ=255)
                                                                                                      tCAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTCCCTCAc                            >  1:409701/1‑143 (MQ=255)
                                                                                                       cAAATCACGCCACAGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa                           <  1:1112423/143‑1 (MQ=255)
                                                                                                                  acaGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCg                <  1:977819/143‑1 (MQ=255)
                                                                                                                    aGCGCGTGGGTCAGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTACGAGATAACAACCgac              >  1:1029155/1‑141 (MQ=255)
                                                                                                                              tCAGGGATTGATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGt    <  2:792722/143‑1 (MQ=255)
                                                                                                                                aGGGATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGATCCACGTAc  >  2:332400/1‑143 (MQ=255)
                                                                                                                                   |                                                                                                                                            
GCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAC‑CCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTAC  >  NC_000913/2661690‑2661960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑