| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,724,099 | T→G | 7.3% | intergenic (+17/‑29) | pssA → / → yfiM | phosphatidylserine synthase/protein YfiM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,724,099 | 0 | T | G | 7.3% | 131.6 / 2.1 | 55 | intergenic (+17/‑29) | pssA/yfiM | phosphatidylserine synthase/protein YfiM |
| Reads supporting (aligned to +/- strand): ref base T (28/21); new base G (3/2); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
CATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATG > NC_000913/2723957‑2724238 | cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTCATCACCACCCCGTCCTGt > 1:440243/1‑143 (MQ=255) aTACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTa > 1:151616/1‑143 (MQ=255) tACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTCTCCGGTGAAGGTTCGTAAACTCATCCGCCGTCTGTGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTACTCACAACCCCGTCCTGCAc > 2:395148/1‑143 (MQ=255) ccgccaGCGTGAGGCGCTATCGTGATCTGCAAAGTTTTGCCGATGATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgg < 2:958189/140‑1 (MQ=255) caTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCCCAACCCCGTCCTGTACGGGgtt > 2:145041/1‑143 (MQ=255) caTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgtt < 1:854972/143‑1 (MQ=255) aTCGTTAAGCAATATCGCGATCTGCAAAGTAGTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgttt < 1:131784/143‑1 (MQ=255) cGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTGGCGCCGTATCCGCATCGCCCGATTAATTATCCGCCTCCTGTACTCCCCTCCCCGCCCTGTCCggggctctt > 1:135807/1‑138 (MQ=255) ttAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgttt < 2:31730/143‑1 (MQ=255) aaGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGttttt > 2:2220/1‑143 (MQ=255) ggaCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTg < 1:795358/141‑1 (MQ=255) gcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGAGCCGTATCCGCATCGACCGAGTAATTAGCCGCATCCTGTAACCAAAACCCCGGCCTGTAGGGGAATTGTACATTGGAgacaaa > 2:1043378/1‑138 (MQ=255) tGCAACGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTCGCGCCGTATCCGCATCGACCGATAAATTAGCCGCATCCTGTACTCACAACACCGTCCTGTACAGCGGTTGTCTTTTCGAGGCCACGTATTg > 2:637313/1‑143 (MQ=255) tGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGCTGGTTTTTTGGAGGCCACGTTTTg > 2:936085/1‑143 (MQ=255) cAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCCCGTTTTGCg > 1:937434/1‑143 (MQ=255) tGCCGATTATCCGGTGAAGGTTCGTAAACGCATCCGCCGATTGCGCGGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttt < 1:516458/143‑1 (MQ=255) ccGATTATCCGGTGAAGGTGCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGTCCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCttttt < 2:855218/143‑1 (MQ=255) gATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGGTTTTTGGACGCCACGCTTTGCGTCTTCttttttt > 1:213075/1‑141 (MQ=255) gATTATCCGGTGAAGGTTCGGAAACTCATCCGCCGTTTGCGCCCTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGt < 1:207489/143‑1 (MQ=255) aaGGTTCGTCAACTCATCCGCCGTTTGCGCCGTATCCGCACCGACGGAGTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAtt < 1:1071232/143‑1 (MQ=255) aaaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTACCCGCATCCTGTAATCACAACACCGTCCTGTACGGGATTTGTTTTTTGGACGCAAAGTTCTGCGTATTATTATCATCTGTTCGCTATTTTTACttcc > 2:474051/1‑141 (MQ=255) aCTCAGCCGCCGTGGTCGCCGTATCCCCGTCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTg < 2:360999/143‑1 (MQ=255) tCATCCGCTGTTTGCTCCGTATTCGCATCGACCGATTTATTGGTCGTATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGa < 1:623581/143‑1 (MQ=255) tCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTCGCCGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTCCTTTCTGGa > 1:423001/1‑143 (MQ=255) ttCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTGTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATg < 1:711130/142‑1 (MQ=255) aTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTCTTGGGGGCCTCGGTTTGCGCATTCTTTTTTTCTGTCCGCTCTTGTTGCTTTCTGGATg > 1:926627/1‑143 (MQ=255) ccgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCTACGTTTTGCGTATTCTTTCTGTCTGTTCGCTATTGTTACTTTCTGGATGCa < 2:592577/143‑1 (MQ=255) gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTCTTGTTACTTTCTGGATGCAGCCATATGGCt > 2:386244/1‑143 (MQ=255) gTATCCACATCGAGCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGTGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAAc < 1:896506/143‑1 (MQ=255) aTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGa < 1:351970/143‑1 (MQ=255) ccGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATa > 2:1026010/1‑143 (MQ=255) ccGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATa > 1:616670/1‑143 (MQ=255) aTCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTg < 1:612858/143‑1 (MQ=255) cGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCgg < 1:607859/143‑1 (MQ=255) gATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCGTTGTGTCTGTTGGATAGGTACACGCTCTGGATGCAGCCATATGGCTAGCGATAGCTGGAGCggg > 1:576428/1‑143 (MQ=255) gATTAATTAGCCGCATCCTGTAATCAAAACACCGTCCTGTAAGGGGCTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCCTATGGCTAACGATAGCTGGAgagcg > 2:687114/1‑139 (MQ=255) ttaattaGTCGCATCCTGTAATACCAACTCCGTCCTGTCCCGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGTCATATGGCTAACGATAGCTGGAGCGGGCa < 2:619442/143‑1 (MQ=255) tccatcaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCa < 1:299554/137‑1 (MQ=255) gCCGCATCCTGTAATCACAACCCCGTCCTGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATaaa < 2:38751/143‑1 (MQ=255) gccTGTTATCACAACCCCGTCCTGTACGTGGTTTGTTTTTTGGATGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCaa < 2:498976/142‑1 (MQ=255) cTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAaca > 1:661416/1‑143 (MQ=255) tGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGCTAAAGCTCAacac > 2:817383/1‑143 (MQ=255) tGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAacac > 1:49165/1‑143 (MQ=255) tAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTCTTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAACGCTCAACACtt > 1:980028/1‑143 (MQ=255) tAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTCTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGCAGCGGGCACGATAAAGCTCAACACtt > 2:996787/1‑143 (MQ=255) aaTCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACttt < 2:533583/143‑1 (MQ=255) aaacaaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTa > 1:966330/6‑143 (MQ=255) tCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTAt > 1:581144/1‑143 (MQ=255) tCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTAt > 2:605082/1‑143 (MQ=255) tCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGACAGGATAAAGCTCAACACTTTAt > 1:746523/1‑143 (MQ=255) acaACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGGTAAAGCTCAACCCTTTATCg > 1:802971/1‑143 (MQ=255) aaaCCCCGTACAGGACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGAAAAAGCTCAACACTTTATCGc > 1:1062179/2‑143 (MQ=255) ccccGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTc > 2:936530/1‑143 (MQ=255) ccccGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTc > 1:1079554/1‑143 (MQ=255) cTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATGGTTGGCTCGGGCATGATAAAGCTCAACACTTTATCGCCTCGGCGATg > 2:233932/1‑143 (MQ=255) cTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATg < 1:389290/143‑1 (MQ=255) | CATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATG > NC_000913/2723957‑2724238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |